Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides
Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively...
Published in: | Chemical Research in Toxicology |
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Online Access: | https://doi.org/10.1021/acs.chemrestox.5b00269 https://archimer.ifremer.fr/doc/00284/39490/44227.pdf https://archimer.ifremer.fr/doc/00284/39490/ |
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fttriple:oai:gotriple.eu:10670/1.jwq159 2023-05-15T15:58:39+02:00 Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides Epelboin, Yanouk Quere, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte https://doi.org/10.1021/acs.chemrestox.5b00269 https://archimer.ifremer.fr/doc/00284/39490/44227.pdf https://archimer.ifremer.fr/doc/00284/39490/ en eng Amer Chemical Soc doi:10.1021/acs.chemrestox.5b00269 10670/1.jwq159 https://archimer.ifremer.fr/doc/00284/39490/44227.pdf https://archimer.ifremer.fr/doc/00284/39490/ Archimer, archive institutionnelle de l'Ifremer Chemical Research In Toxicology (0893-228X) (Amer Chemical Soc), 2015-09 , Vol. 28 , N. 9 , P. 1831-1841 envir psy Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1021/acs.chemrestox.5b00269 2023-01-22T16:35:15Z Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. Text Crassostrea gigas Pacific oyster Unknown Pacific Chemical Research in Toxicology 28 9 1831 1841 |
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envir psy Epelboin, Yanouk Quere, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
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envir psy |
description |
Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. |
format |
Text |
author |
Epelboin, Yanouk Quere, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_facet |
Epelboin, Yanouk Quere, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_sort |
Epelboin, Yanouk |
title |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_short |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_fullStr |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full_unstemmed |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_sort |
energy and antioxidant responses of pacific oyster exposed to trace levels of pesticides |
publisher |
Amer Chemical Soc |
url |
https://doi.org/10.1021/acs.chemrestox.5b00269 https://archimer.ifremer.fr/doc/00284/39490/44227.pdf https://archimer.ifremer.fr/doc/00284/39490/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Chemical Research In Toxicology (0893-228X) (Amer Chemical Soc), 2015-09 , Vol. 28 , N. 9 , P. 1831-1841 |
op_relation |
doi:10.1021/acs.chemrestox.5b00269 10670/1.jwq159 https://archimer.ifremer.fr/doc/00284/39490/44227.pdf https://archimer.ifremer.fr/doc/00284/39490/ |
op_doi |
https://doi.org/10.1021/acs.chemrestox.5b00269 |
container_title |
Chemical Research in Toxicology |
container_volume |
28 |
container_issue |
9 |
container_start_page |
1831 |
op_container_end_page |
1841 |
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1766394427543126016 |