Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. T...
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fttriple:oai:gotriple.eu:10670/1.jv80os 2023-05-15T15:57:58+02:00 Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas Boullot, Floriane Castrec, Justine Bidault, Adeline Dantas, Natanael Payton, Laura Perrigault, Mickael Tran, Damien Amzil, Zouher Boudry, Pierre Soudant, Philippe Hegaret, Helene Fabioux, Caroline https://doi.org/10.3390/md15010021 https://archimer.ifremer.fr/doc/00368/47877/47892.pdf https://archimer.ifremer.fr/doc/00368/47877/ en eng Mdpi Ag doi:10.3390/md15010021 10670/1.jv80os https://archimer.ifremer.fr/doc/00368/47877/47892.pdf https://archimer.ifremer.fr/doc/00368/47877/ lic_creative-commons other Archimer, archive institutionnelle de l'Ifremer Marine Drugs (1660-3397) (Mdpi Ag), 2017-01 , Vol. 15 , N. 1 , P. 21 (1-23) envir info Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.3390/md15010021 2023-01-22T17:56:49Z Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters. Text Crassostrea gigas Pacific oyster Unknown Pacific Marine Drugs 15 1 21 |
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envir info Boullot, Floriane Castrec, Justine Bidault, Adeline Dantas, Natanael Payton, Laura Perrigault, Mickael Tran, Damien Amzil, Zouher Boudry, Pierre Soudant, Philippe Hegaret, Helene Fabioux, Caroline Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
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Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters. |
format |
Text |
author |
Boullot, Floriane Castrec, Justine Bidault, Adeline Dantas, Natanael Payton, Laura Perrigault, Mickael Tran, Damien Amzil, Zouher Boudry, Pierre Soudant, Philippe Hegaret, Helene Fabioux, Caroline |
author_facet |
Boullot, Floriane Castrec, Justine Bidault, Adeline Dantas, Natanael Payton, Laura Perrigault, Mickael Tran, Damien Amzil, Zouher Boudry, Pierre Soudant, Philippe Hegaret, Helene Fabioux, Caroline |
author_sort |
Boullot, Floriane |
title |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
title_short |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
title_full |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas |
title_sort |
molecular characterization of voltage-gated sodium channels and their relations with paralytic shellfish toxin bioaccumulation in the pacific oyster crassostrea gigas |
publisher |
Mdpi Ag |
url |
https://doi.org/10.3390/md15010021 https://archimer.ifremer.fr/doc/00368/47877/47892.pdf https://archimer.ifremer.fr/doc/00368/47877/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Marine Drugs (1660-3397) (Mdpi Ag), 2017-01 , Vol. 15 , N. 1 , P. 21 (1-23) |
op_relation |
doi:10.3390/md15010021 10670/1.jv80os https://archimer.ifremer.fr/doc/00368/47877/47892.pdf https://archimer.ifremer.fr/doc/00368/47877/ |
op_rights |
lic_creative-commons other |
op_doi |
https://doi.org/10.3390/md15010021 |
container_title |
Marine Drugs |
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15 |
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1 |
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21 |
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1766393691277099008 |