Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Made...
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fttriple:oai:gotriple.eu:10670/1.jpj3nm 2023-05-15T17:41:29+02:00 Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp 2020-01-01 https://doi.org/10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://archimer.ifremer.fr/doc/00624/73630/ en eng MDPI AG doi:10.3390/toxins12040267 10670/1.jpj3nm https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://archimer.ifremer.fr/doc/00624/73630/ other Archimer, archive institutionnelle de l'Ifremer Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.) anthro-se archeo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ 2020 fttriple https://doi.org/10.3390/toxins12040267 2023-01-22T17:28:44Z Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). Text Northeast Atlantic Unknown Toxins 12 4 267 |
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anthro-se archeo Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
topic_facet |
anthro-se archeo |
description |
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). |
format |
Text |
author |
Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp |
author_facet |
Estevez, Pablo Sibat, Manoella Leão-martins, José Manuel Reis Costa, Pedro Gago-martínez, Ana Hess, Philipp |
author_sort |
Estevez, Pablo |
title |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_short |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_full |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_fullStr |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_full_unstemmed |
Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
title_sort |
liquid chromatography coupled to high-resolution mass spectrometry for the confirmation of caribbean ciguatoxin-1 as the main toxin responsible for ciguatera poisoning caused by fish from european atlantic coasts |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/toxins12040267 https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://archimer.ifremer.fr/doc/00624/73630/ |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Archimer, archive institutionnelle de l'Ifremer Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.) |
op_relation |
doi:10.3390/toxins12040267 10670/1.jpj3nm https://archimer.ifremer.fr/doc/00624/73630/73067.pdf https://archimer.ifremer.fr/doc/00624/73630/73068.pdf https://archimer.ifremer.fr/doc/00624/73630/ |
op_rights |
other |
op_doi |
https://doi.org/10.3390/toxins12040267 |
container_title |
Toxins |
container_volume |
12 |
container_issue |
4 |
container_start_page |
267 |
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1766143078854295552 |