Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals wer...
Published in: | Journal of Proteomics |
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Online Access: | https://doi.org/10.1016/j.jprot.2014.06.030 https://hal.archives-ouvertes.fr/hal-02548704 |
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fttriple:oai:gotriple.eu:10670/1.jp3ncf 2023-05-15T15:57:52+02:00 Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) 2014-01-01 https://doi.org/10.1016/j.jprot.2014.06.030 https://hal.archives-ouvertes.fr/hal-02548704 en eng HAL CCSD Elsevier hal-02548704 doi:10.1016/j.jprot.2014.06.030 10670/1.jp3ncf https://hal.archives-ouvertes.fr/hal-02548704 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics Journal of Proteomics, Elsevier, 2014, 109, pp.176--187. ⟨10.1016/j.jprot.2014.06.030⟩ Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus envir stat Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2014 fttriple https://doi.org/10.1016/j.jprot.2014.06.030 2023-01-22T17:27:21Z International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. (C) 2014 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Journal of Proteomics 109 176 187 |
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Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus envir stat |
spellingShingle |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus envir stat Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
topic_facet |
Warburg ACL Crassostrea gigas Metabolism Marine bivalves Herpesvirus envir stat |
description |
International audience Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 mu Var) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. (C) 2014 Elsevier B.V. All rights reserved. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) |
format |
Article in Journal/Newspaper |
author |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie |
author_facet |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quéré, Claudie Madec, Stéphanie |
author_sort |
Corporeau, Charlotte |
title |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_short |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_fullStr |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full_unstemmed |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_sort |
proteomic signatures of the oyster metabolic response to herpesvirus oshv-1 μvar infection |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://doi.org/10.1016/j.jprot.2014.06.030 https://hal.archives-ouvertes.fr/hal-02548704 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1874-3919 EISSN: 1876-7737 Journal of Proteomics Journal of Proteomics, Elsevier, 2014, 109, pp.176--187. ⟨10.1016/j.jprot.2014.06.030⟩ |
op_relation |
hal-02548704 doi:10.1016/j.jprot.2014.06.030 10670/1.jp3ncf https://hal.archives-ouvertes.fr/hal-02548704 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.jprot.2014.06.030 |
container_title |
Journal of Proteomics |
container_volume |
109 |
container_start_page |
176 |
op_container_end_page |
187 |
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1766393582508310528 |