Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out usin...
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fttriple:oai:gotriple.eu:10670/1.jbtgjr 2023-05-15T15:58:06+02:00 Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas Da Costa Gonzalez, Fiz Robert, Rene Quere, Claudie Wikfors, Gary H. Soudant, Philippe https://doi.org/10.1007/s11745-015-4006-z https://archimer.ifremer.fr/doc/00268/37962/36086.pdf https://archimer.ifremer.fr/doc/00268/37962/ en eng Springer Heidelberg doi:10.1007/s11745-015-4006-z 10670/1.jbtgjr https://archimer.ifremer.fr/doc/00268/37962/36086.pdf https://archimer.ifremer.fr/doc/00268/37962/ other Archimer, archive institutionnelle de l'Ifremer Lipids (0024-4201) (Springer Heidelberg), 2015-05 , Vol. 50 , N. 5 , P. 503-511 envir anthro-se Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1007/s11745-015-4006-z 2023-01-22T16:56:10Z Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply. Text Crassostrea gigas Unknown Lipids 50 5 503 511 |
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envir anthro-se Da Costa Gonzalez, Fiz Robert, Rene Quere, Claudie Wikfors, Gary H. Soudant, Philippe Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
topic_facet |
envir anthro-se |
description |
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply. |
format |
Text |
author |
Da Costa Gonzalez, Fiz Robert, Rene Quere, Claudie Wikfors, Gary H. Soudant, Philippe |
author_facet |
Da Costa Gonzalez, Fiz Robert, Rene Quere, Claudie Wikfors, Gary H. Soudant, Philippe |
author_sort |
Da Costa Gonzalez, Fiz |
title |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
title_short |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
title_full |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
title_fullStr |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
title_full_unstemmed |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas |
title_sort |
essential fatty acid assimilation and synthesis in larvae of the bivalve crassostrea gigas |
publisher |
Springer Heidelberg |
url |
https://doi.org/10.1007/s11745-015-4006-z https://archimer.ifremer.fr/doc/00268/37962/36086.pdf https://archimer.ifremer.fr/doc/00268/37962/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Archimer, archive institutionnelle de l'Ifremer Lipids (0024-4201) (Springer Heidelberg), 2015-05 , Vol. 50 , N. 5 , P. 503-511 |
op_relation |
doi:10.1007/s11745-015-4006-z 10670/1.jbtgjr https://archimer.ifremer.fr/doc/00268/37962/36086.pdf https://archimer.ifremer.fr/doc/00268/37962/ |
op_rights |
other |
op_doi |
https://doi.org/10.1007/s11745-015-4006-z |
container_title |
Lipids |
container_volume |
50 |
container_issue |
5 |
container_start_page |
503 |
op_container_end_page |
511 |
_version_ |
1766393827286843392 |