Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera
International audience The genomics of economically important marine bivalves is studied to provide better understanding of the molecular mechanisms underlying their different reproductive strategies. The recently available gonad transcriptome of the black-lip pearl oyster Pinctada margaritifera is...
Published in: | PLOS ONE |
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Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2015
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Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0122819 https://hal.archives-ouvertes.fr/hal-01684919 |
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fttriple:oai:gotriple.eu:10670/1.ii5vre |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACL BIOMINERALIZATION DIFFERENTIATION C-ELEGANS PACIFIC OYSTER TEMPERATURE FOXL2 GAMETOGENESIS GERM-CELL DEVELOPMENT CRASSOSTREA-GIGAS GENE-EXPRESSION Biominéralisation Gamétogénèse Déterminants sexuels Huître perlière stat psy |
spellingShingle |
ACL BIOMINERALIZATION DIFFERENTIATION C-ELEGANS PACIFIC OYSTER TEMPERATURE FOXL2 GAMETOGENESIS GERM-CELL DEVELOPMENT CRASSOSTREA-GIGAS GENE-EXPRESSION Biominéralisation Gamétogénèse Déterminants sexuels Huître perlière stat psy Teaniniuraitemoana, Vaihiti Huvet, Arnaud Levy, Peva Gaertner-Mazouni, Nabila Gueguen, Yannick Le Moullac, Gilles Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
topic_facet |
ACL BIOMINERALIZATION DIFFERENTIATION C-ELEGANS PACIFIC OYSTER TEMPERATURE FOXL2 GAMETOGENESIS GERM-CELL DEVELOPMENT CRASSOSTREA-GIGAS GENE-EXPRESSION Biominéralisation Gamétogénèse Déterminants sexuels Huître perlière stat psy |
description |
International audience The genomics of economically important marine bivalves is studied to provide better understanding of the molecular mechanisms underlying their different reproductive strategies. The recently available gonad transcriptome of the black-lip pearl oyster Pinctada margaritifera is a novel and powerful resource to study these mechanisms in marine mollusks displaying hermaphroditic features. In this study, RNAseq quantification data of the P. margaritifera gonad transcriptome were analyzed to identify candidate genes in histologically-characterized gonad samples to provide molecular signatures of the female and male sexual pathway in this pearl oyster. Based on the RNAseq data set, stringent expression analysis identified 1,937 contigs that were differentially expressed between the gonad histological categories. From the hierarchical clustering analysis, a new reproduction model is proposed, based on a dual histo-molecular analytical approach. Nine candidate genes were identified as markers of the sexual pathway: 7 for the female pathway and 2 for the male one. Their mRNA levels were assayed by real-time PCR on a new set of gonadic samples. A clustering method revealed four principal expression patterns based on the relative gene expression ratio. A multivariate regression tree realized on these new samples and validated on the previously analyzed RNAseq samples showed that the sexual pathway of P. margaritifera can be predicted by a 3-gene-pair expression ratio model of 4 different genes: pmarg-43476, pmarg-foxl2, pmarg-54338 and pmarg-fem1-like. This 3-gene-pair expression ratio model strongly suggests only the implication of pmarg-foxl2 and pmarg-fem1-like in the sex inversion of P. margaritifera. This work provides the first histo-molecular model of P. margaritifera reproduction and a gene expression signature of its sexual pathway discriminating the male and female pathways. These represent useful tools for understanding and studying sex inversion, sex differentiation and sex determinism in ... |
author2 |
Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecologie des systèmes marins côtiers (Ecosym) Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) ANR-11-AGRO-0006,POLYPERL,Gestion intégrée et adaptation de la perliculture en Polynésie française dans le contexte du changement global : approche environnementale, économique et sociale(2011) |
format |
Article in Journal/Newspaper |
author |
Teaniniuraitemoana, Vaihiti Huvet, Arnaud Levy, Peva Gaertner-Mazouni, Nabila Gueguen, Yannick Le Moullac, Gilles |
author_facet |
Teaniniuraitemoana, Vaihiti Huvet, Arnaud Levy, Peva Gaertner-Mazouni, Nabila Gueguen, Yannick Le Moullac, Gilles |
author_sort |
Teaniniuraitemoana, Vaihiti |
title |
Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
title_short |
Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
title_full |
Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
title_fullStr |
Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
title_full_unstemmed |
Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera |
title_sort |
molecular signatures discriminating the male and the female sexual pathways in the pearl oyster pinctada margaritifera |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0122819 https://hal.archives-ouvertes.fr/hal-01684919 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0122819. ⟨10.1371/journal.pone.0122819⟩ |
op_relation |
hal-01684919 doi:10.1371/journal.pone.0122819 IRD: PAR00013062 10670/1.ii5vre https://hal.archives-ouvertes.fr/hal-01684919 |
op_rights |
other |
op_doi |
https://doi.org/10.1371/journal.pone.0122819 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
3 |
container_start_page |
e0122819 |
_version_ |
1766394939774599168 |
spelling |
fttriple:oai:gotriple.eu:10670/1.ii5vre 2023-05-15T15:59:09+02:00 Molecular Signatures Discriminating the Male and the Female Sexual Pathways in the Pearl Oyster Pinctada margaritifera Teaniniuraitemoana, Vaihiti Huvet, Arnaud Levy, Peva Gaertner-Mazouni, Nabila Gueguen, Yannick Le Moullac, Gilles Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecologie des systèmes marins côtiers (Ecosym) Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) ANR-11-AGRO-0006,POLYPERL,Gestion intégrée et adaptation de la perliculture en Polynésie française dans le contexte du changement global : approche environnementale, économique et sociale(2011) 2015-01-01 https://doi.org/10.1371/journal.pone.0122819 https://hal.archives-ouvertes.fr/hal-01684919 en eng HAL CCSD Public Library of Science hal-01684919 doi:10.1371/journal.pone.0122819 IRD: PAR00013062 10670/1.ii5vre https://hal.archives-ouvertes.fr/hal-01684919 other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0122819. ⟨10.1371/journal.pone.0122819⟩ ACL BIOMINERALIZATION DIFFERENTIATION C-ELEGANS PACIFIC OYSTER TEMPERATURE FOXL2 GAMETOGENESIS GERM-CELL DEVELOPMENT CRASSOSTREA-GIGAS GENE-EXPRESSION Biominéralisation Gamétogénèse Déterminants sexuels Huître perlière stat psy Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1371/journal.pone.0122819 2023-01-22T18:13:55Z International audience The genomics of economically important marine bivalves is studied to provide better understanding of the molecular mechanisms underlying their different reproductive strategies. The recently available gonad transcriptome of the black-lip pearl oyster Pinctada margaritifera is a novel and powerful resource to study these mechanisms in marine mollusks displaying hermaphroditic features. In this study, RNAseq quantification data of the P. margaritifera gonad transcriptome were analyzed to identify candidate genes in histologically-characterized gonad samples to provide molecular signatures of the female and male sexual pathway in this pearl oyster. Based on the RNAseq data set, stringent expression analysis identified 1,937 contigs that were differentially expressed between the gonad histological categories. From the hierarchical clustering analysis, a new reproduction model is proposed, based on a dual histo-molecular analytical approach. Nine candidate genes were identified as markers of the sexual pathway: 7 for the female pathway and 2 for the male one. Their mRNA levels were assayed by real-time PCR on a new set of gonadic samples. A clustering method revealed four principal expression patterns based on the relative gene expression ratio. A multivariate regression tree realized on these new samples and validated on the previously analyzed RNAseq samples showed that the sexual pathway of P. margaritifera can be predicted by a 3-gene-pair expression ratio model of 4 different genes: pmarg-43476, pmarg-foxl2, pmarg-54338 and pmarg-fem1-like. This 3-gene-pair expression ratio model strongly suggests only the implication of pmarg-foxl2 and pmarg-fem1-like in the sex inversion of P. margaritifera. This work provides the first histo-molecular model of P. margaritifera reproduction and a gene expression signature of its sexual pathway discriminating the male and female pathways. These represent useful tools for understanding and studying sex inversion, sex differentiation and sex determinism in ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific PLOS ONE 10 3 e0122819 |