Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another...
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fttriple:oai:gotriple.eu:10670/1.f2ttjn 2023-05-15T15:58:28+02:00 Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jerome Robert, Rene Nicolas, Jean-louis https://doi.org/10.1016/j.aquaculture.2016.07.020 https://archimer.ifremer.fr/doc/00346/45691/45311.pdf https://archimer.ifremer.fr/doc/00346/45691/ en eng Elsevier Science Bv doi:10.1016/j.aquaculture.2016.07.020 10670/1.f2ttjn https://archimer.ifremer.fr/doc/00346/45691/45311.pdf https://archimer.ifremer.fr/doc/00346/45691/ other Archimer, archive institutionnelle de l'Ifremer Aquaculture (0044-8486) (Elsevier Science Bv), 2016-11 , Vol. 464 , P. 434-444 envir geo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1016/j.aquaculture.2016.07.020 2023-01-22T16:42:54Z This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1–V3 region of 16S rRNA, were highly similar (50–65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (~ 90%), with 47% Rhodobacteraceae (α-Proteobacteria). γ-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (β-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 ± 7%), 42 OTUs of α-Proteobacteria (19 ± 7%) and 26 of γ-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 ± 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3–12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of Rhodobacteraceae, Rhizobiales and Planctomycetes. Only a few nitrite oxidisers were detected ... Text Crassostrea gigas Pacific oyster Unknown Pacific Aquaculture 464 434 444 |
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envir geo Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jerome Robert, Rene Nicolas, Jean-louis Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
topic_facet |
envir geo |
description |
This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1–V3 region of 16S rRNA, were highly similar (50–65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (~ 90%), with 47% Rhodobacteraceae (α-Proteobacteria). γ-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (β-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 ± 7%), 42 OTUs of α-Proteobacteria (19 ± 7%) and 26 of γ-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 ± 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3–12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of Rhodobacteraceae, Rhizobiales and Planctomycetes. Only a few nitrite oxidisers were detected ... |
format |
Text |
author |
Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jerome Robert, Rene Nicolas, Jean-louis |
author_facet |
Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jerome Robert, Rene Nicolas, Jean-louis |
author_sort |
Asmani, Katia |
title |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_short |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_full |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_fullStr |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_sort |
establishment of microbiota in larval culture of pacific oyster, crassostrea gigas |
publisher |
Elsevier Science Bv |
url |
https://doi.org/10.1016/j.aquaculture.2016.07.020 https://archimer.ifremer.fr/doc/00346/45691/45311.pdf https://archimer.ifremer.fr/doc/00346/45691/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Aquaculture (0044-8486) (Elsevier Science Bv), 2016-11 , Vol. 464 , P. 434-444 |
op_relation |
doi:10.1016/j.aquaculture.2016.07.020 10670/1.f2ttjn https://archimer.ifremer.fr/doc/00346/45691/45311.pdf https://archimer.ifremer.fr/doc/00346/45691/ |
op_rights |
other |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.07.020 |
container_title |
Aquaculture |
container_volume |
464 |
container_start_page |
434 |
op_container_end_page |
444 |
_version_ |
1766394222000209920 |