Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress
International audience Groups of oysters (Crassostrea gigas) were exposed to 25 degrees C for 24 days (controls to 13 degrees C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 degrees C above the summer seawater temperature encountered i...
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Online Access: | https://doi.org/10.1111/j.1742-4658.2007.06156.x https://hal.univ-brest.fr/hal-00669953 |
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fttriple:oai:gotriple.eu:10670/1.ej8d2l 2023-05-15T15:57:39+02:00 Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress Meistertzheim, Anne-Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie-Therese Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2007-01-01 https://doi.org/10.1111/j.1742-4658.2007.06156.x https://hal.univ-brest.fr/hal-00669953 en eng HAL CCSD Wiley hal-00669953 doi:10.1111/j.1742-4658.2007.06156.x 10670/1.ej8d2l https://hal.univ-brest.fr/hal-00669953 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal FEBS Journal, Wiley, 2007, 274 (24), pp.6392-6402. ⟨10.1111/j.1742-4658.2007.06156.x⟩ climate Crassostrea gigas gene expression heat stress prolonged thermal stress geo info Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2007 fttriple https://doi.org/10.1111/j.1742-4658.2007.06156.x 2023-01-22T16:49:12Z International audience Groups of oysters (Crassostrea gigas) were exposed to 25 degrees C for 24 days (controls to 13 degrees C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 degrees C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up- and downregulated genes in gill and mantle tissues after 7-10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real-time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3-7 days of exposure, followed by a decrease at 14 days, and a second, less-pronounced increase at 17-24 days. A slow-down in protein synthesis occurred after 24 days of thermal stress. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific FEBS Journal 274 24 6392 6402 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
climate Crassostrea gigas gene expression heat stress prolonged thermal stress geo info |
spellingShingle |
climate Crassostrea gigas gene expression heat stress prolonged thermal stress geo info Meistertzheim, Anne-Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie-Therese Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
topic_facet |
climate Crassostrea gigas gene expression heat stress prolonged thermal stress geo info |
description |
International audience Groups of oysters (Crassostrea gigas) were exposed to 25 degrees C for 24 days (controls to 13 degrees C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 degrees C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. Suppression subtractive hybridization was used to identify specific up- and downregulated genes in gill and mantle tissues after 7-10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real-time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal stress. Expression of most transcripts (mainly heat shock proteins and genes involved in cellular homeostasis) showed a high and rapid increase at 3-7 days of exposure, followed by a decrease at 14 days, and a second, less-pronounced increase at 17-24 days. A slow-down in protein synthesis occurred after 24 days of thermal stress. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Meistertzheim, Anne-Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie-Therese |
author_facet |
Meistertzheim, Anne-Leila Tanguy, Arnaud Moraga, Dario Thébault, Marie-Therese |
author_sort |
Meistertzheim, Anne-Leila |
title |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
title_short |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
title_full |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
title_fullStr |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
title_full_unstemmed |
Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress |
title_sort |
identification of differentially expressed genes of the pacific oyster crassostrea gigas exposed to prolonged thermal stress |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://doi.org/10.1111/j.1742-4658.2007.06156.x https://hal.univ-brest.fr/hal-00669953 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal FEBS Journal, Wiley, 2007, 274 (24), pp.6392-6402. ⟨10.1111/j.1742-4658.2007.06156.x⟩ |
op_relation |
hal-00669953 doi:10.1111/j.1742-4658.2007.06156.x 10670/1.ej8d2l https://hal.univ-brest.fr/hal-00669953 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1111/j.1742-4658.2007.06156.x |
container_title |
FEBS Journal |
container_volume |
274 |
container_issue |
24 |
container_start_page |
6392 |
op_container_end_page |
6402 |
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1766393326187053056 |