High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon
In the present study we detected unicellular diazotrophic cyanobacteria along a nutrient gradient in New Caledonia's coral lagoon (24 000 km(2)) using whole-cell hybridization of specific Nitro821 probes (TSA-FISH, Tyramide Signal Amplification of Fluorescent In Situ Hybridization). The specifi...
Published in: | Aquatic Microbial Ecology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://doi.org/10.3354/ame01185 https://hal.archives-ouvertes.fr/hal-00727031 |
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fttriple:oai:gotriple.eu:10670/1.ct4i9n 2023-05-15T17:34:21+02:00 High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon Biegala, Isabelle C. Raimbault, Patrick Caractérisation et modélisation des échanges dans des lagons soumis aux influences terrigènes et anthropiques (CAMELIA) Institut de Recherche pour le Développement (IRD Nouvelle-Calédonie )-Institut de Recherche pour le Développement (IRD Guyane ) 2008-01-01 https://doi.org/10.3354/ame01185 https://hal.archives-ouvertes.fr/hal-00727031 en eng HAL CCSD Inter Research hal-00727031 doi:10.3354/ame01185 10670/1.ct4i9n https://hal.archives-ouvertes.fr/hal-00727031 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0948-3055 Aquatic Microbial Ecology Aquatic Microbial Ecology, Inter Research, 2008, 51, pp.45-53. ⟨10.3354/ame01185⟩ diazotrophs picoplankton FISH Pacific coral lagoon symbiosis 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBES TROPICAL NORTH-ATLANTIC IN-SITU IDENTIFICATION NITROGEN-FIXATION CONFOCAL MICROSCOPY COMMUNITY STRUCTURE N-2 FIXATION OCEAN CYANOBACTERIA envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2008 fttriple https://doi.org/10.3354/ame01185 2023-01-22T18:49:40Z In the present study we detected unicellular diazotrophic cyanobacteria along a nutrient gradient in New Caledonia's coral lagoon (24 000 km(2)) using whole-cell hybridization of specific Nitro821 probes (TSA-FISH, Tyramide Signal Amplification of Fluorescent In Situ Hybridization). The specificity of this probe was confirmed on cultures and in the natural environment, Surprisingly, the community of Nitro821-targeted cells was numerically dominated by picocyanobacteria (97%, 1 to 1.5 mu m). These organisms were either free living (63%), recovered with the 0.2 to 3 mu m size fraction or associated (37%) to particles or planktonic dinoflagellates from larger size fractions (3 to 10 mu m and >10 mu m). Diazotrophic picocyanobacterial abundance ranged from 3 to 140 cells ml(-1) along the nutrient gradient and was highest at the oligotrophic lagoonal station. These cells may contribute to N-2 fixation from the 10 mu m size fraction (0 to 1.9 nmol N-1 24 h(-1)). In addition, in the middle of the lagoon daylight N-15(2) fixation could explain 78% of N-15(2) fixation over 24 h. The results presented in the present study reveal for the first time a significant concentration of photosynthetic diazotrophs within the marine picoplankton community. Article in Journal/Newspaper North Atlantic Unknown Pacific Aquatic Microbial Ecology 51 45 53 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
diazotrophs picoplankton FISH Pacific coral lagoon symbiosis 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBES TROPICAL NORTH-ATLANTIC IN-SITU IDENTIFICATION NITROGEN-FIXATION CONFOCAL MICROSCOPY COMMUNITY STRUCTURE N-2 FIXATION OCEAN CYANOBACTERIA envir socio |
spellingShingle |
diazotrophs picoplankton FISH Pacific coral lagoon symbiosis 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBES TROPICAL NORTH-ATLANTIC IN-SITU IDENTIFICATION NITROGEN-FIXATION CONFOCAL MICROSCOPY COMMUNITY STRUCTURE N-2 FIXATION OCEAN CYANOBACTERIA envir socio Biegala, Isabelle C. Raimbault, Patrick High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
topic_facet |
diazotrophs picoplankton FISH Pacific coral lagoon symbiosis 16S RIBOSOMAL-RNA TARGETED OLIGONUCLEOTIDE PROBES TROPICAL NORTH-ATLANTIC IN-SITU IDENTIFICATION NITROGEN-FIXATION CONFOCAL MICROSCOPY COMMUNITY STRUCTURE N-2 FIXATION OCEAN CYANOBACTERIA envir socio |
description |
In the present study we detected unicellular diazotrophic cyanobacteria along a nutrient gradient in New Caledonia's coral lagoon (24 000 km(2)) using whole-cell hybridization of specific Nitro821 probes (TSA-FISH, Tyramide Signal Amplification of Fluorescent In Situ Hybridization). The specificity of this probe was confirmed on cultures and in the natural environment, Surprisingly, the community of Nitro821-targeted cells was numerically dominated by picocyanobacteria (97%, 1 to 1.5 mu m). These organisms were either free living (63%), recovered with the 0.2 to 3 mu m size fraction or associated (37%) to particles or planktonic dinoflagellates from larger size fractions (3 to 10 mu m and >10 mu m). Diazotrophic picocyanobacterial abundance ranged from 3 to 140 cells ml(-1) along the nutrient gradient and was highest at the oligotrophic lagoonal station. These cells may contribute to N-2 fixation from the 10 mu m size fraction (0 to 1.9 nmol N-1 24 h(-1)). In addition, in the middle of the lagoon daylight N-15(2) fixation could explain 78% of N-15(2) fixation over 24 h. The results presented in the present study reveal for the first time a significant concentration of photosynthetic diazotrophs within the marine picoplankton community. |
author2 |
Caractérisation et modélisation des échanges dans des lagons soumis aux influences terrigènes et anthropiques (CAMELIA) Institut de Recherche pour le Développement (IRD Nouvelle-Calédonie )-Institut de Recherche pour le Développement (IRD Guyane ) |
format |
Article in Journal/Newspaper |
author |
Biegala, Isabelle C. Raimbault, Patrick |
author_facet |
Biegala, Isabelle C. Raimbault, Patrick |
author_sort |
Biegala, Isabelle C. |
title |
High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
title_short |
High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
title_full |
High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
title_fullStr |
High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
title_full_unstemmed |
High abundance of diazotrophic picocyanobacteria (< 3 mu m) in a Southwest Pacific coral lagoon |
title_sort |
high abundance of diazotrophic picocyanobacteria (< 3 mu m) in a southwest pacific coral lagoon |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://doi.org/10.3354/ame01185 https://hal.archives-ouvertes.fr/hal-00727031 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0948-3055 Aquatic Microbial Ecology Aquatic Microbial Ecology, Inter Research, 2008, 51, pp.45-53. ⟨10.3354/ame01185⟩ |
op_relation |
hal-00727031 doi:10.3354/ame01185 10670/1.ct4i9n https://hal.archives-ouvertes.fr/hal-00727031 |
op_rights |
undefined |
op_doi |
https://doi.org/10.3354/ame01185 |
container_title |
Aquatic Microbial Ecology |
container_volume |
51 |
container_start_page |
45 |
op_container_end_page |
53 |
_version_ |
1766133157164220416 |