The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas
International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Online Access: | https://doi.org/10.1016/j.cbpa.2011.05.011 https://hal.univ-brest.fr/hal-00670286 |
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fttriple:oai:gotriple.eu:10670/1.c6vsht 2023-05-15T15:58:08+02:00 The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas Séguineau, Catherine Racotta, Llie, Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Programa de Acuicultura CIBNOR Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2011-01-01 https://doi.org/10.1016/j.cbpa.2011.05.011 https://hal.univ-brest.fr/hal-00670286 en eng HAL CCSD Elsevier hal-00670286 doi:10.1016/j.cbpa.2011.05.011 10670/1.c6vsht https://hal.univ-brest.fr/hal-00670286 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2011, 160 (1), pp.87-93. ⟨10.1016/j.cbpa.2011.05.011⟩ Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2011 fttriple https://doi.org/10.1016/j.cbpa.2011.05.011 2023-01-22T18:32:05Z International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 160 1 87 93 |
institution |
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Unknown |
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fttriple |
language |
English |
topic |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production envir socio |
spellingShingle |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production envir socio Séguineau, Catherine Racotta, Llie, Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
topic_facet |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production envir socio |
description |
International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Programa de Acuicultura CIBNOR Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Séguineau, Catherine Racotta, Llie, Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe |
author_facet |
Séguineau, Catherine Racotta, Llie, Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe |
author_sort |
Séguineau, Catherine |
title |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_short |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_full |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_fullStr |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_sort |
influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://doi.org/10.1016/j.cbpa.2011.05.011 https://hal.univ-brest.fr/hal-00670286 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, Elsevier, 2011, 160 (1), pp.87-93. ⟨10.1016/j.cbpa.2011.05.011⟩ |
op_relation |
hal-00670286 doi:10.1016/j.cbpa.2011.05.011 10670/1.c6vsht https://hal.univ-brest.fr/hal-00670286 |
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op_doi |
https://doi.org/10.1016/j.cbpa.2011.05.011 |
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Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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