A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from...
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fttriple:oai:gotriple.eu:10670/1.boyw7g 2023-05-15T13:58:54+02:00 A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. 2015-01-01 https://doi.org/10.7939/R3794173N https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a en eng doi:10.7939/R3794173N 10670/1.boyw7g https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a other ERA : Education and Research Archive geo class Other https://vocabularies.coar-repositories.org/resource_types/c_1843/ 2015 fttriple https://doi.org/10.7939/R3794173N 2023-01-22T16:32:32Z A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from Candida antarctica immobilized onto the large surface area of the monolith. The microreactor was used for the room temperature ethanolysis of TAG from edible oils including canola, sesame, soybean and refined-bleached-deodorized palm oil. GC/MS-NCI and GC/FID were used to prove the identification of the FAEE and FAME products. The microreactor completely transformed the starting oils into FAEEs or FAMEs, without the use of any reagents other than alcohol, in quantities suitable for GC analysis. The prototype microreactors were reusable >5 times with ethanol and 2 times with methanol. The FAEE products obtained using the microreactor were similar to those produced using commercial Novozyme 435 enzyme beads as well as by catalysis with ethanolic H2SO4. Other/Unknown Material Antarc* Antarctica Unknown Fid ENVELOPE(-65.939,-65.939,-68.664,-68.664) Monolith ENVELOPE(163.283,163.283,-66.950,-66.950) Monolith The ENVELOPE(163.283,163.283,-66.950,-66.950) The Monolith ENVELOPE(-57.955,-57.955,-63.897,-63.897) |
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English |
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geo class Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
topic_facet |
geo class |
description |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols (TAG) into fatty acid ethyl ester (FAEE) or fatty acid methyl ester (FAME) derivatives was developed. The microreactor was a porous silica monolith fabricated within a 320 μm ID fused silica capillary with lipase from Candida antarctica immobilized onto the large surface area of the monolith. The microreactor was used for the room temperature ethanolysis of TAG from edible oils including canola, sesame, soybean and refined-bleached-deodorized palm oil. GC/MS-NCI and GC/FID were used to prove the identification of the FAEE and FAME products. The microreactor completely transformed the starting oils into FAEEs or FAMEs, without the use of any reagents other than alcohol, in quantities suitable for GC analysis. The prototype microreactors were reusable >5 times with ethanol and 2 times with methanol. The FAEE products obtained using the microreactor were similar to those produced using commercial Novozyme 435 enzyme beads as well as by catalysis with ethanolic H2SO4. |
format |
Other/Unknown Material |
author |
Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. |
author_facet |
Mugo, Samuel M. Anuar, Sabiqah T. Curtis, Jonathan M. |
author_sort |
Mugo, Samuel M. |
title |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_short |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_full |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_fullStr |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_full_unstemmed |
A flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for GC analysis |
title_sort |
flow-through enzymatic microreactor for the rapid conversion of triacylglycerols into fatty acid ethyl ester and fatty acid methyl ester derivatives for gc analysis |
publishDate |
2015 |
url |
https://doi.org/10.7939/R3794173N https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a |
long_lat |
ENVELOPE(-65.939,-65.939,-68.664,-68.664) ENVELOPE(163.283,163.283,-66.950,-66.950) ENVELOPE(163.283,163.283,-66.950,-66.950) ENVELOPE(-57.955,-57.955,-63.897,-63.897) |
geographic |
Fid Monolith Monolith The The Monolith |
geographic_facet |
Fid Monolith Monolith The The Monolith |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ERA : Education and Research Archive |
op_relation |
doi:10.7939/R3794173N 10670/1.boyw7g https://era.library.ualberta.ca/items/1006a99d-e102-4bd6-bf51-63d3c69c563a |
op_rights |
other |
op_doi |
https://doi.org/10.7939/R3794173N |
_version_ |
1766267277076856832 |