Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes
International audience Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial co...
Published in: | Environmental Microbiology |
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Online Access: | https://doi.org/10.1111/1462-2920.14621 https://hal.archives-ouvertes.fr/hal-02381765/file/Debeljak2019EnvMicrobiol.pdf https://hal.archives-ouvertes.fr/hal-02381765 |
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fttriple:oai:gotriple.eu:10670/1.bavjfj 2023-05-15T18:24:45+02:00 Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stéphane Obernosterer, Ingrid Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Leibniz-Institut für Ostseeforschung Warnemünde (IOW) Leibniz Association 2019-01-01 https://doi.org/10.1111/1462-2920.14621 https://hal.archives-ouvertes.fr/hal-02381765/file/Debeljak2019EnvMicrobiol.pdf https://hal.archives-ouvertes.fr/hal-02381765 en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell hal-02381765 doi:10.1111/1462-2920.14621 10670/1.bavjfj https://hal.archives-ouvertes.fr/hal-02381765/file/Debeljak2019EnvMicrobiol.pdf https://hal.archives-ouvertes.fr/hal-02381765 other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, 21 (7), pp.2360-2374. ⟨10.1111/1462-2920.14621⟩ envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2019 fttriple https://doi.org/10.1111/1462-2920.14621 2023-01-22T16:41:13Z International audience Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial communities from high nutrient low chlorophyll and naturally Fe‐fertilized waters in the Southern Ocean. We observed major differences in the contribution of prokaryotic groups at different taxonomic levels to transcripts encoding Fe‐uptake mechanisms, intracellular Fe storage and replacement and Fe‐related pathways in the tricarboxylic acid (TCA) cycle. The composition of the prokaryotic communities contributing to the transcripts of a given Fe‐related pathway was overall independent of the in situ Fe supply, indicating that microbial taxa utilize distinct Fe‐related metabolic processes. Only a few prokaryotic groups contributed to the transcripts of more than one Fe‐uptake mechanism, suggesting limited metabolic versatility. Taxa‐specific expression of individual genes varied among prokaryotic groups and was substantially higher for all inspected genes in Fe‐limited as compared to naturally fertilized waters, indicating the link between transcriptional state and Fe regime. Different metabolic strategies regarding low Fe concentrations in the Southern Ocean are discussed for two abundant prokaryotic groups, Pelagibacteraceae and Flavobacteriaceae. Article in Journal/Newspaper Southern Ocean Unknown Southern Ocean Environmental Microbiology 21 7 2360 2374 |
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envir geo |
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envir geo Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stéphane Obernosterer, Ingrid Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
topic_facet |
envir geo |
description |
International audience Iron (Fe) is a limiting nutrient in large regions of the ocean, but the strategies of prokaryotes to cope with this micronutrient are poorly known. Using a gene‐specific approach from metatranscriptomics data, we investigated seven Fe‐related metabolic pathways in microbial communities from high nutrient low chlorophyll and naturally Fe‐fertilized waters in the Southern Ocean. We observed major differences in the contribution of prokaryotic groups at different taxonomic levels to transcripts encoding Fe‐uptake mechanisms, intracellular Fe storage and replacement and Fe‐related pathways in the tricarboxylic acid (TCA) cycle. The composition of the prokaryotic communities contributing to the transcripts of a given Fe‐related pathway was overall independent of the in situ Fe supply, indicating that microbial taxa utilize distinct Fe‐related metabolic processes. Only a few prokaryotic groups contributed to the transcripts of more than one Fe‐uptake mechanism, suggesting limited metabolic versatility. Taxa‐specific expression of individual genes varied among prokaryotic groups and was substantially higher for all inspected genes in Fe‐limited as compared to naturally fertilized waters, indicating the link between transcriptional state and Fe regime. Different metabolic strategies regarding low Fe concentrations in the Southern Ocean are discussed for two abundant prokaryotic groups, Pelagibacteraceae and Flavobacteriaceae. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interactions Hôtes-Pathogènes-Environnements (IHPE) Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD) Leibniz-Institut für Ostseeforschung Warnemünde (IOW) Leibniz Association |
format |
Article in Journal/Newspaper |
author |
Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stéphane Obernosterer, Ingrid |
author_facet |
Debeljak, Pavla Toulza, Eve Beier, Sara Blain, Stéphane Obernosterer, Ingrid |
author_sort |
Debeljak, Pavla |
title |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_short |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_full |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_fullStr |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_full_unstemmed |
Microbial iron metabolism as revealed by gene expression profiles in contrasted Southern Ocean regimes |
title_sort |
microbial iron metabolism as revealed by gene expression profiles in contrasted southern ocean regimes |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://doi.org/10.1111/1462-2920.14621 https://hal.archives-ouvertes.fr/hal-02381765/file/Debeljak2019EnvMicrobiol.pdf https://hal.archives-ouvertes.fr/hal-02381765 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, 21 (7), pp.2360-2374. ⟨10.1111/1462-2920.14621⟩ |
op_relation |
hal-02381765 doi:10.1111/1462-2920.14621 10670/1.bavjfj https://hal.archives-ouvertes.fr/hal-02381765/file/Debeljak2019EnvMicrobiol.pdf https://hal.archives-ouvertes.fr/hal-02381765 |
op_rights |
other |
op_doi |
https://doi.org/10.1111/1462-2920.14621 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
7 |
container_start_page |
2360 |
op_container_end_page |
2374 |
_version_ |
1766205609728802816 |