High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas
We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including t...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://doi.org/10.1016/j.dci.2014.12.002 https://archimer.ifremer.fr/doc/00239/35009/33545.pdf https://archimer.ifremer.fr/doc/00239/35009/ |
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fttriple:oai:gotriple.eu:10670/1.axmfyo 2023-05-15T15:57:57+02:00 High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne https://doi.org/10.1016/j.dci.2014.12.002 https://archimer.ifremer.fr/doc/00239/35009/33545.pdf https://archimer.ifremer.fr/doc/00239/35009/ en eng Elsevier doi:10.1016/j.dci.2014.12.002 10670/1.axmfyo https://archimer.ifremer.fr/doc/00239/35009/33545.pdf https://archimer.ifremer.fr/doc/00239/35009/ Archimer, archive institutionnelle de l'Ifremer Developmental & Comparative Immunology (0145-305X) (Elsevier), 2015-04 , Vol. 49 , N. 2 , P. 231-238 envir archeo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1016/j.dci.2014.12.002 2023-01-22T17:08:56Z We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses. Text Crassostrea gigas Unknown Developmental & Comparative Immunology 49 2 231 238 |
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envir archeo Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
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We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses. |
format |
Text |
author |
Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne |
author_facet |
Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne |
author_sort |
Rosa, Rafael D. |
title |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_short |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_full |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_fullStr |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_full_unstemmed |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_sort |
high polymorphism in big defensin gene expression reveals presence-absence gene variability (pav) in the oyster crassostrea gigas |
publisher |
Elsevier |
url |
https://doi.org/10.1016/j.dci.2014.12.002 https://archimer.ifremer.fr/doc/00239/35009/33545.pdf https://archimer.ifremer.fr/doc/00239/35009/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Archimer, archive institutionnelle de l'Ifremer Developmental & Comparative Immunology (0145-305X) (Elsevier), 2015-04 , Vol. 49 , N. 2 , P. 231-238 |
op_relation |
doi:10.1016/j.dci.2014.12.002 10670/1.axmfyo https://archimer.ifremer.fr/doc/00239/35009/33545.pdf https://archimer.ifremer.fr/doc/00239/35009/ |
op_doi |
https://doi.org/10.1016/j.dci.2014.12.002 |
container_title |
Developmental & Comparative Immunology |
container_volume |
49 |
container_issue |
2 |
container_start_page |
231 |
op_container_end_page |
238 |
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1766393673461792768 |