Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean
International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of u...
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Online Access: | https://doi.org/10.1038/srep04661 https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://hal.sorbonne-universite.fr/hal-01358986 |
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fttriple:oai:gotriple.eu:10670/1.8gtcec 2023-05-15T14:47:46+02:00 Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-ERic Lovejoy, Connie Université Laval Québec (ULaval) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2014-04-11 https://doi.org/10.1038/srep04661 https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://hal.sorbonne-universite.fr/hal-01358986 en eng HAL CCSD Nature Publishing Group hal-01358986 doi:10.1038/srep04661 10670/1.8gtcec https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://hal.sorbonne-universite.fr/hal-01358986 lic_creative-commons other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports Scientific Reports, Nature Publishing Group, 2014, 4, pp.4661. ⟨10.1038/srep04661⟩ geo envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2014 fttriple https://doi.org/10.1038/srep04661 2023-01-22T18:13:24Z International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. Article in Journal/Newspaper Arctic Arctic Ocean Unknown Arctic Arctic Ocean Scientific Reports 4 1 |
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geo envir Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-ERic Lovejoy, Connie Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
topic_facet |
geo envir |
description |
International audience Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean. |
author2 |
Université Laval Québec (ULaval) Laboratoire d'Ecogéochimie des environnements benthiques (LECOB) Observatoire océanologique de Banyuls (OOB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-ERic Lovejoy, Connie |
author_facet |
Pedneault, Estelle Galand, Pierre E Potvin, Marianne Tremblay, Jean-ERic Lovejoy, Connie |
author_sort |
Pedneault, Estelle |
title |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_short |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_fullStr |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_full_unstemmed |
Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean |
title_sort |
archaeal amoa and urec genes and their transcriptional activity in the arctic ocean |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://doi.org/10.1038/srep04661 https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://hal.sorbonne-universite.fr/hal-01358986 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports Scientific Reports, Nature Publishing Group, 2014, 4, pp.4661. ⟨10.1038/srep04661⟩ |
op_relation |
hal-01358986 doi:10.1038/srep04661 10670/1.8gtcec https://hal.sorbonne-universite.fr/hal-01358986/file/srep04661.pdf https://hal.sorbonne-universite.fr/hal-01358986 |
op_rights |
lic_creative-commons other |
op_doi |
https://doi.org/10.1038/srep04661 |
container_title |
Scientific Reports |
container_volume |
4 |
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1 |
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1766318848915537920 |