The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean
International audience The tricarboxylic acid (TCA) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation in...
Published in: | Environmental Microbiology Reports |
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Online Access: | https://doi.org/10.1111/1758-2229.12267 https://hal.sorbonne-universite.fr/hal-01130266/file/Beier_2015_The_transcriptional.pdf https://hal.sorbonne-universite.fr/hal-01130266 |
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fttriple:oai:gotriple.eu:10670/1.6v90s7 2023-05-15T18:25:05+02:00 The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean Beier, Sara Galvez, Maria J Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Universidad de Concepción Chile Universidad de Playa Ancha Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institute for Marine and Antarctic Studies Horbat (IMAS) University of Tasmania Hobart, Australia (UTAS) Antarctic Climate and Ecosystems Cooperative Research Centre (ACE-CRC) ANR-10-BLAN-0614,KEOPS 2,Kerguelen : Comparaison plateau Ocean2(2010) 2015-06-01 https://doi.org/10.1111/1758-2229.12267 https://hal.sorbonne-universite.fr/hal-01130266/file/Beier_2015_The_transcriptional.pdf https://hal.sorbonne-universite.fr/hal-01130266 en eng HAL CCSD Wiley hal-01130266 doi:10.1111/1758-2229.12267 10670/1.6v90s7 https://hal.sorbonne-universite.fr/hal-01130266/file/Beier_2015_The_transcriptional.pdf https://hal.sorbonne-universite.fr/hal-01130266 Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1758-2229 Environmental Microbiology Reports Environmental Microbiology Reports, Wiley, 2015, 7 (3), pp.427-434. ⟨10.1111/1758-2229.12267⟩ PHYTOPLANKTON BLOOM HETEROTROPHIC BACTERIA GROWTH BACTERIOPLANKTON PROTEORHODOPSIN METABOLISM LIMITATION GENOME SEA ACL envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1111/1758-2229.12267 2023-01-22T17:27:31Z International audience The tricarboxylic acid (TCA) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation into cell material instead of being released as carbon dioxide. Despite the importance for the fate of organic matter, the environmental factors that induce the glyoxylate cycle in microbial communities remain poorly understood. In this study, we assessed the expression of isocitrate lyase, the enzyme that induces the switch to the glyoxylate cycle, of the ubiquitous SAR11 clade in response to natural iron fertilization in the Southern Ocean. The cell-specific transcriptional regulation of the glyoxylate cycle, as determined by the ratio between copy numbers of isocitrate lyase gene transcripts and isocitrate genes, was consistently lower in iron fertilized than in high-nutrient, low chlorophyll waters (by 2.4-to 16.5-fold). SAR11 cell-specific isocitrate lyase gene transcription was negatively correlated to chlorophyll a, and bulk bacterial heterotrophic metabolism. We conclude that the glyoxylate cycle is a metabolic strategy for SAR11 that is highly sensitive to the degree of iron and carbon limitation in the marine environment. Article in Journal/Newspaper Southern Ocean Unknown Southern Ocean Environmental Microbiology Reports 7 3 427 434 |
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Open Polar |
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Unknown |
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fttriple |
language |
English |
topic |
PHYTOPLANKTON BLOOM HETEROTROPHIC BACTERIA GROWTH BACTERIOPLANKTON PROTEORHODOPSIN METABOLISM LIMITATION GENOME SEA ACL envir socio |
spellingShingle |
PHYTOPLANKTON BLOOM HETEROTROPHIC BACTERIA GROWTH BACTERIOPLANKTON PROTEORHODOPSIN METABOLISM LIMITATION GENOME SEA ACL envir socio Beier, Sara Galvez, Maria J Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
topic_facet |
PHYTOPLANKTON BLOOM HETEROTROPHIC BACTERIA GROWTH BACTERIOPLANKTON PROTEORHODOPSIN METABOLISM LIMITATION GENOME SEA ACL envir socio |
description |
International audience The tricarboxylic acid (TCA) cycle is a central metabolic pathway that is present in all aerobic organisms and initiates the respiration of organic material. The glyoxylate cycle is a variation of the TCA cycle, where organic material is recycled for subsequent assimilation into cell material instead of being released as carbon dioxide. Despite the importance for the fate of organic matter, the environmental factors that induce the glyoxylate cycle in microbial communities remain poorly understood. In this study, we assessed the expression of isocitrate lyase, the enzyme that induces the switch to the glyoxylate cycle, of the ubiquitous SAR11 clade in response to natural iron fertilization in the Southern Ocean. The cell-specific transcriptional regulation of the glyoxylate cycle, as determined by the ratio between copy numbers of isocitrate lyase gene transcripts and isocitrate genes, was consistently lower in iron fertilized than in high-nutrient, low chlorophyll waters (by 2.4-to 16.5-fold). SAR11 cell-specific isocitrate lyase gene transcription was negatively correlated to chlorophyll a, and bulk bacterial heterotrophic metabolism. We conclude that the glyoxylate cycle is a metabolic strategy for SAR11 that is highly sensitive to the degree of iron and carbon limitation in the marine environment. |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Universidad de Concepción Chile Universidad de Playa Ancha Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institute for Marine and Antarctic Studies Horbat (IMAS) University of Tasmania Hobart, Australia (UTAS) Antarctic Climate and Ecosystems Cooperative Research Centre (ACE-CRC) ANR-10-BLAN-0614,KEOPS 2,Kerguelen : Comparaison plateau Ocean2(2010) |
format |
Article in Journal/Newspaper |
author |
Beier, Sara Galvez, Maria J Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid |
author_facet |
Beier, Sara Galvez, Maria J Molina, Veronica Sarthou, Géraldine Quéroué, Fabien Blain, Stephane Obernosterer, Ingrid |
author_sort |
Beier, Sara |
title |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_short |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_full |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_fullStr |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_full_unstemmed |
The transcriptional regulation of the glyoxylate cycle in SAR11 in response to iron fertilization in the Southern Ocean |
title_sort |
transcriptional regulation of the glyoxylate cycle in sar11 in response to iron fertilization in the southern ocean |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://doi.org/10.1111/1758-2229.12267 https://hal.sorbonne-universite.fr/hal-01130266/file/Beier_2015_The_transcriptional.pdf https://hal.sorbonne-universite.fr/hal-01130266 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1758-2229 Environmental Microbiology Reports Environmental Microbiology Reports, Wiley, 2015, 7 (3), pp.427-434. ⟨10.1111/1758-2229.12267⟩ |
op_relation |
hal-01130266 doi:10.1111/1758-2229.12267 10670/1.6v90s7 https://hal.sorbonne-universite.fr/hal-01130266/file/Beier_2015_The_transcriptional.pdf https://hal.sorbonne-universite.fr/hal-01130266 |
op_doi |
https://doi.org/10.1111/1758-2229.12267 |
container_title |
Environmental Microbiology Reports |
container_volume |
7 |
container_issue |
3 |
container_start_page |
427 |
op_container_end_page |
434 |
_version_ |
1766206261893791744 |