Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean)
International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure...
Published in: | Limnology and Oceanography: Methods |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://doi.org/10.4319/lom.2012.10.483 https://hal.archives-ouvertes.fr/hal-00707656 |
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fttriple:oai:gotriple.eu:10670/1.6k5vr9 2023-05-15T16:05:37+02:00 Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) Xing, Xiaogang Claustre, Hervé Blain, Stéphane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Centre d'études biologiques de Chizé (CEBC) Centre National de la Recherche Scientifique (CNRS) 2012-01-01 https://doi.org/10.4319/lom.2012.10.483 https://hal.archives-ouvertes.fr/hal-00707656 en eng HAL CCSD Association for the Sciences of Limnology and Oceanography hal-00707656 doi:10.4319/lom.2012.10.483 10670/1.6k5vr9 https://hal.archives-ouvertes.fr/hal-00707656 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1541-5856 Limnology and Oceanography: Methods Limnology and Oceanography: Methods, Association for the Sciences of Limnology and Oceanography, 2012, 10, pp.483-495. ⟨10.4319/lom.2012.10.483⟩ envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2012 fttriple https://doi.org/10.4319/lom.2012.10.483 2023-01-22T16:44:47Z International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. Article in Journal/Newspaper Elephant Seals Southern Ocean Unknown Kerguelen Southern Ocean Limnology and Oceanography: Methods 10 7 483 495 |
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English |
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envir geo Xing, Xiaogang Claustre, Hervé Blain, Stéphane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
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envir geo |
description |
International audience As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. |
author2 |
Laboratoire d'océanographie de Villefranche (LOV) Observatoire océanologique de Villefranche-sur-mer (OOVM) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Centre d'études biologiques de Chizé (CEBC) Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Xing, Xiaogang Claustre, Hervé Blain, Stéphane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe |
author_facet |
Xing, Xiaogang Claustre, Hervé Blain, Stéphane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe |
author_sort |
Xing, Xiaogang |
title |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_short |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_fullStr |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full_unstemmed |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_sort |
quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the kerguelen region (southern ocean) |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://doi.org/10.4319/lom.2012.10.483 https://hal.archives-ouvertes.fr/hal-00707656 |
geographic |
Kerguelen Southern Ocean |
geographic_facet |
Kerguelen Southern Ocean |
genre |
Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Ocean |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1541-5856 Limnology and Oceanography: Methods Limnology and Oceanography: Methods, Association for the Sciences of Limnology and Oceanography, 2012, 10, pp.483-495. ⟨10.4319/lom.2012.10.483⟩ |
op_relation |
hal-00707656 doi:10.4319/lom.2012.10.483 10670/1.6k5vr9 https://hal.archives-ouvertes.fr/hal-00707656 |
op_rights |
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op_doi |
https://doi.org/10.4319/lom.2012.10.483 |
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Limnology and Oceanography: Methods |
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10 |
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7 |
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483 |
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495 |
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