Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean)
As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predep...
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fttriple:oai:gotriple.eu:10670/1.59oqzx 2023-05-15T16:05:37+02:00 Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) Xing, Xiaogang Claustre, Herve Blain, Stephane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe https://doi.org/10.4319/lom.2012.10.483 https://archimer.ifremer.fr/doc/00248/35965/36927.pdf https://archimer.ifremer.fr/doc/00248/35965/ en eng Amer Soc Limnology Oceanography doi:10.4319/lom.2012.10.483 10670/1.59oqzx https://archimer.ifremer.fr/doc/00248/35965/36927.pdf https://archimer.ifremer.fr/doc/00248/35965/ other Archimer, archive institutionnelle de l'Ifremer Limnology And Oceanography-methods (1541-5856) (Amer Soc Limnology Oceanography), 2012-07 , Vol. 10 , P. 483-495 envir geo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.4319/lom.2012.10.483 2023-01-22T18:25:26Z As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. Text Elephant Seals Southern Ocean Unknown Kerguelen Southern Ocean Limnology and Oceanography: Methods 10 7 483 495 |
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envir geo Xing, Xiaogang Claustre, Herve Blain, Stephane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
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envir geo |
description |
As the proxy for Chlorophyll a (Chl a) concentration, thousands of fluorescence profiles were measured by instrumented elephant seals in the Kerguelen region (Southern Ocean). For accurate retrieval of Chl a concentrations acquired by in vivo fluorometer, a two-step procedure is applied: 1) A predeployment intercalibration with accurate determination by high performance liquid chromatography (HPLC) analysis, which not only calibrates fluorescence in appropriate Chl a concentration units, but also strongly reduces variability between fluorometers, and 2) a profile-by-profile quenching correction analysis, which effectively eliminates the fluorescence quenching issue at surface around noon, and results in consistent profiles between day and night. The quenching correction is conducted through an extrapolation of the deep fluorescence value toward surface. As proved by a validation procedure in the Western Mediterranean Sea, the correction method is practical and relatively reliable when there is no credible reference, especially for deep mixed waters, as in the Southern Ocean. Even in the shallow mixed waters, the method is also effective in reducing the influence of quenching. |
format |
Text |
author |
Xing, Xiaogang Claustre, Herve Blain, Stephane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe |
author_facet |
Xing, Xiaogang Claustre, Herve Blain, Stephane D'Ortenzio, Fabrizio, Antoine, David Ras, Josephine Guinet, Christophe |
author_sort |
Xing, Xiaogang |
title |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_short |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_fullStr |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_full_unstemmed |
Quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: A case study with instrumented elephant seals in the Kerguelen region (Southern Ocean) |
title_sort |
quenching correction for in vivo chlorophyll fluorescence acquired by autonomous platforms: a case study with instrumented elephant seals in the kerguelen region (southern ocean) |
publisher |
Amer Soc Limnology Oceanography |
url |
https://doi.org/10.4319/lom.2012.10.483 https://archimer.ifremer.fr/doc/00248/35965/36927.pdf https://archimer.ifremer.fr/doc/00248/35965/ |
geographic |
Kerguelen Southern Ocean |
geographic_facet |
Kerguelen Southern Ocean |
genre |
Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Ocean |
op_source |
Archimer, archive institutionnelle de l'Ifremer Limnology And Oceanography-methods (1541-5856) (Amer Soc Limnology Oceanography), 2012-07 , Vol. 10 , P. 483-495 |
op_relation |
doi:10.4319/lom.2012.10.483 10670/1.59oqzx https://archimer.ifremer.fr/doc/00248/35965/36927.pdf https://archimer.ifremer.fr/doc/00248/35965/ |
op_rights |
other |
op_doi |
https://doi.org/10.4319/lom.2012.10.483 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
10 |
container_issue |
7 |
container_start_page |
483 |
op_container_end_page |
495 |
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1766401519784034304 |