Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles
While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of...
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fttriple:oai:gotriple.eu:10670/1.3x3hlx 2023-05-15T15:58:07+02:00 Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles Gonzalez-fernandez, Carmen Tallec, Kevin Le Goic, Nelly Lambert, Christophe Soudant, Philippe Huvet, Arnaud Suquet, Marc Berchel, Mathieu Paul-pont, Ika https://doi.org/10.1016/j.chemosphere.2018.06.039 https://archimer.ifremer.fr/doc/00443/55459/56976.pdf https://archimer.ifremer.fr/doc/00443/55459/ en eng Pergamon-elsevier Science Ltd doi:10.1016/j.chemosphere.2018.06.039 10670/1.3x3hlx https://archimer.ifremer.fr/doc/00443/55459/56976.pdf https://archimer.ifremer.fr/doc/00443/55459/ other Archimer, archive institutionnelle de l'Ifremer Chemosphere (0045-6535) (Pergamon-elsevier Science Ltd), 2018-10 , Vol. 208 , P. 764-772 envir socio Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1016/j.chemosphere.2018.06.039 2023-01-22T17:19:38Z While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the adhesion of NPs to oyster spermatozoa and oocytes as suggested by the increase of relative cell size and complexity measured by flow-cytometry and confirmed by microscopy observations. A significant increase of ROS production was observed in sperm cells upon exposure to 100 mg L−1 PS-COOH, but was not observed with PS-NH2, suggesting a differential effect according to the NP-associated functional group. Altogether, these results demonstrate that the effects of NPs occur rapidly, are complex and are possibly associated with the cellular eco-corona, which could modify NPs behaviour and toxicity. Text Crassostrea gigas Pacific oyster Unknown Pacific Chemosphere 208 764 772 |
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envir socio |
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envir socio Gonzalez-fernandez, Carmen Tallec, Kevin Le Goic, Nelly Lambert, Christophe Soudant, Philippe Huvet, Arnaud Suquet, Marc Berchel, Mathieu Paul-pont, Ika Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
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envir socio |
description |
While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the adhesion of NPs to oyster spermatozoa and oocytes as suggested by the increase of relative cell size and complexity measured by flow-cytometry and confirmed by microscopy observations. A significant increase of ROS production was observed in sperm cells upon exposure to 100 mg L−1 PS-COOH, but was not observed with PS-NH2, suggesting a differential effect according to the NP-associated functional group. Altogether, these results demonstrate that the effects of NPs occur rapidly, are complex and are possibly associated with the cellular eco-corona, which could modify NPs behaviour and toxicity. |
format |
Text |
author |
Gonzalez-fernandez, Carmen Tallec, Kevin Le Goic, Nelly Lambert, Christophe Soudant, Philippe Huvet, Arnaud Suquet, Marc Berchel, Mathieu Paul-pont, Ika |
author_facet |
Gonzalez-fernandez, Carmen Tallec, Kevin Le Goic, Nelly Lambert, Christophe Soudant, Philippe Huvet, Arnaud Suquet, Marc Berchel, Mathieu Paul-pont, Ika |
author_sort |
Gonzalez-fernandez, Carmen |
title |
Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
title_short |
Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
title_full |
Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
title_fullStr |
Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
title_full_unstemmed |
Cellular responses of Pacific oyster ( Crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
title_sort |
cellular responses of pacific oyster ( crassostrea gigas ) gametes exposed in vitro to polystyrene nanoparticles |
publisher |
Pergamon-elsevier Science Ltd |
url |
https://doi.org/10.1016/j.chemosphere.2018.06.039 https://archimer.ifremer.fr/doc/00443/55459/56976.pdf https://archimer.ifremer.fr/doc/00443/55459/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Chemosphere (0045-6535) (Pergamon-elsevier Science Ltd), 2018-10 , Vol. 208 , P. 764-772 |
op_relation |
doi:10.1016/j.chemosphere.2018.06.039 10670/1.3x3hlx https://archimer.ifremer.fr/doc/00443/55459/56976.pdf https://archimer.ifremer.fr/doc/00443/55459/ |
op_rights |
other |
op_doi |
https://doi.org/10.1016/j.chemosphere.2018.06.039 |
container_title |
Chemosphere |
container_volume |
208 |
container_start_page |
764 |
op_container_end_page |
772 |
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