Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France
Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was inve...
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fttriple:oai:gotriple.eu:10670/1.3d8bj6 2023-05-15T15:58:01+02:00 Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France Azandegbe, Afi Garnier, Matthieu Andrieux, Francoise Kerouel, Roger Philippon, Xavier Nicolas, Jean-louis 2010-01-01 https://doi.org/10.3354/dao02253 https://archimer.ifremer.fr/doc/00017/12813/9756.pdf https://archimer.ifremer.fr/doc/00017/12813/ en eng Inter-research doi:10.3354/dao02253 10670/1.3d8bj6 https://archimer.ifremer.fr/doc/00017/12813/9756.pdf https://archimer.ifremer.fr/doc/00017/12813/ other Archimer, archive institutionnelle de l'Ifremer Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2010-09 , Vol. 91 , N. 3 , P. 213-221 envir geo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ 2010 fttriple https://doi.org/10.3354/dao02253 2023-01-22T18:52:11Z Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V. aestuarianus in sediment when it became unculturable. Both total Vibrio spp. and V. aestuarianus had a seasonal trend. The highest concentrations were recovered in the warmest months. Its abundance ranged from 10(2) to 4 x 10(5) CFU ml(-1) in haemolymph and from 10(3) to 1 x 10(4) CFU g(-1) in the sediment. Temperature was the main factor influencing the concentration of Vibrio spp. and V. aestuarianus in the sediment. Thus V aestuarianus might subsist during the cold seasons in the sediment, from which it can emerge when environmental conditions became favourable. Text Crassostrea gigas Pacific oyster Unknown Pacific Diseases of Aquatic Organisms 91 3 213 221 |
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envir geo Azandegbe, Afi Garnier, Matthieu Andrieux, Francoise Kerouel, Roger Philippon, Xavier Nicolas, Jean-louis Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
topic_facet |
envir geo |
description |
Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V. aestuarianus in sediment when it became unculturable. Both total Vibrio spp. and V. aestuarianus had a seasonal trend. The highest concentrations were recovered in the warmest months. Its abundance ranged from 10(2) to 4 x 10(5) CFU ml(-1) in haemolymph and from 10(3) to 1 x 10(4) CFU g(-1) in the sediment. Temperature was the main factor influencing the concentration of Vibrio spp. and V. aestuarianus in the sediment. Thus V aestuarianus might subsist during the cold seasons in the sediment, from which it can emerge when environmental conditions became favourable. |
format |
Text |
author |
Azandegbe, Afi Garnier, Matthieu Andrieux, Francoise Kerouel, Roger Philippon, Xavier Nicolas, Jean-louis |
author_facet |
Azandegbe, Afi Garnier, Matthieu Andrieux, Francoise Kerouel, Roger Philippon, Xavier Nicolas, Jean-louis |
author_sort |
Azandegbe, Afi |
title |
Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
title_short |
Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
title_full |
Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
title_fullStr |
Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
title_full_unstemmed |
Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France |
title_sort |
occurrence and seasonality of vibrio aestuarianus in sediment and crassostrea gigas haemolymph at two oyster farms in france |
publisher |
Inter-research |
publishDate |
2010 |
url |
https://doi.org/10.3354/dao02253 https://archimer.ifremer.fr/doc/00017/12813/9756.pdf https://archimer.ifremer.fr/doc/00017/12813/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2010-09 , Vol. 91 , N. 3 , P. 213-221 |
op_relation |
doi:10.3354/dao02253 10670/1.3d8bj6 https://archimer.ifremer.fr/doc/00017/12813/9756.pdf https://archimer.ifremer.fr/doc/00017/12813/ |
op_rights |
other |
op_doi |
https://doi.org/10.3354/dao02253 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
91 |
container_issue |
3 |
container_start_page |
213 |
op_container_end_page |
221 |
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1766393731338993664 |