Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify cr...
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fttriple:oai:gotriple.eu:10670/1.2t9f4t 2023-05-15T15:58:46+02:00 Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc 2015-02-01 https://doi.org/10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://archimer.ifremer.fr/doc/00239/35010/ en eng Elsevier Science Bv doi:10.1016/j.aquaculture.2014.11.025 10670/1.2t9f4t https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://archimer.ifremer.fr/doc/00239/35010/ other Archimer, archive institutionnelle de l'Ifremer Aquaculture (0044-8486) (Elsevier Science Bv), 2015-02-01 , Vol. 437 , P. 201-207 envir socio Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ 2015 fttriple https://doi.org/10.1016/j.aquaculture.2014.11.025 2023-01-22T18:27:33Z Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1 to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R2 = 0.237) and with trochophore Velocity of the Average Path (VAP; R2 = 0.255) and negatively correlated with oocyte total steryl esters (R2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R2 = 0.352), highlighting that the prediction of embryo development in molluscs is a complex process requiring sophisticated multi-factor models. Text Crassostrea gigas Pacific oyster Unknown Pacific Aquaculture 437 201 207 |
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envir socio |
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envir socio Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
topic_facet |
envir socio |
description |
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1 to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R2 = 0.237) and with trochophore Velocity of the Average Path (VAP; R2 = 0.255) and negatively correlated with oocyte total steryl esters (R2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R2 = 0.352), highlighting that the prediction of embryo development in molluscs is a complex process requiring sophisticated multi-factor models. |
format |
Text |
author |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_facet |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_short |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_fullStr |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_sort |
assessment of oocyte and trochophore quality in pacific oyster, crassostrea gigas |
publisher |
Elsevier Science Bv |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://archimer.ifremer.fr/doc/00239/35010/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Aquaculture (0044-8486) (Elsevier Science Bv), 2015-02-01 , Vol. 437 , P. 201-207 |
op_relation |
doi:10.1016/j.aquaculture.2014.11.025 10670/1.2t9f4t https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://archimer.ifremer.fr/doc/00239/35010/ |
op_rights |
other |
op_doi |
https://doi.org/10.1016/j.aquaculture.2014.11.025 |
container_title |
Aquaculture |
container_volume |
437 |
container_start_page |
201 |
op_container_end_page |
207 |
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1766394546491490304 |