Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata
International audience Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their ow...
Main Authors: | , , , , , , , , , , , , , |
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Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2017
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Online Access: | https://doi.org/10.1371/journal.pone.0173315.s011 https://hal.sorbonne-universite.fr/hal-01587420/file/journal.pone.0173315.pdf https://hal.sorbonne-universite.fr/hal-01587420 |
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Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
envir geo |
spellingShingle |
envir geo Ritter, Andrés, Cabioch, Léa Brillet-Guéguen, Loraine, Corre, Erwan, Cosse, Audrey, Dartevelle, Laurence, Duruflé, Harold, Fasshauer, Carina, Goulitquer, Sophie, Thomas, François Correa, Juan, Potin, Philippe, Faugeron, Sylvain, Leblanc, Catherine Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
topic_facet |
envir geo |
description |
International audience Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular ... |
author2 |
Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Pontificia Universidad Católica de Chile (UC) Diatom Genomics LCQB (LCQB-DG) Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Evolutionary Biology and Ecology of Algae (EBEA) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Universidad Austral de Chile-Centre National de la Recherche Scientifique (CNRS)-Pontificia Universidad Católica de Chile (UC) |
format |
Article in Journal/Newspaper |
author |
Ritter, Andrés, Cabioch, Léa Brillet-Guéguen, Loraine, Corre, Erwan, Cosse, Audrey, Dartevelle, Laurence, Duruflé, Harold, Fasshauer, Carina, Goulitquer, Sophie, Thomas, François Correa, Juan, Potin, Philippe, Faugeron, Sylvain, Leblanc, Catherine |
author_facet |
Ritter, Andrés, Cabioch, Léa Brillet-Guéguen, Loraine, Corre, Erwan, Cosse, Audrey, Dartevelle, Laurence, Duruflé, Harold, Fasshauer, Carina, Goulitquer, Sophie, Thomas, François Correa, Juan, Potin, Philippe, Faugeron, Sylvain, Leblanc, Catherine |
author_sort |
Ritter, Andrés, |
title |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
title_short |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
title_full |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
title_fullStr |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
title_full_unstemmed |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata |
title_sort |
herbivore-induced chemical and molecular responses of the kelps laminaria digitata and lessonia spicata |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0173315.s011 https://hal.sorbonne-universite.fr/hal-01587420/file/journal.pone.0173315.pdf https://hal.sorbonne-universite.fr/hal-01587420 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (3), pp.e0173315. ⟨10.1371/journal.pone.0173315.s011⟩ |
op_relation |
hal-01587420 doi:10.1371/journal.pone.0173315.s011 10670/1.2sw3ej https://hal.sorbonne-universite.fr/hal-01587420/file/journal.pone.0173315.pdf https://hal.sorbonne-universite.fr/hal-01587420 |
op_rights |
lic_creative-commons other |
op_doi |
https://doi.org/10.1371/journal.pone.0173315.s011 |
_version_ |
1766134375191150592 |
spelling |
fttriple:oai:gotriple.eu:10670/1.2sw3ej 2023-05-15T17:35:16+02:00 Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata Ritter, Andrés, Cabioch, Léa Brillet-Guéguen, Loraine, Corre, Erwan, Cosse, Audrey, Dartevelle, Laurence, Duruflé, Harold, Fasshauer, Carina, Goulitquer, Sophie, Thomas, François Correa, Juan, Potin, Philippe, Faugeron, Sylvain, Leblanc, Catherine Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Pontificia Universidad Católica de Chile (UC) Diatom Genomics LCQB (LCQB-DG) Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Evolutionary Biology and Ecology of Algae (EBEA) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Universidad Austral de Chile-Centre National de la Recherche Scientifique (CNRS)-Pontificia Universidad Católica de Chile (UC) 2017-01-01 https://doi.org/10.1371/journal.pone.0173315.s011 https://hal.sorbonne-universite.fr/hal-01587420/file/journal.pone.0173315.pdf https://hal.sorbonne-universite.fr/hal-01587420 en eng HAL CCSD Public Library of Science hal-01587420 doi:10.1371/journal.pone.0173315.s011 10670/1.2sw3ej https://hal.sorbonne-universite.fr/hal-01587420/file/journal.pone.0173315.pdf https://hal.sorbonne-universite.fr/hal-01587420 lic_creative-commons other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (3), pp.e0173315. ⟨10.1371/journal.pone.0173315.s011⟩ envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2017 fttriple https://doi.org/10.1371/journal.pone.0173315.s011 2023-01-22T17:11:15Z International audience Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular ... Article in Journal/Newspaper North Atlantic Unknown Pacific |