Amino-nanopolystyrene exposures of oyster (Crassostrea gigas) embryos induced no apparent intergenerational effects

• Published in: Nanotoxicology
• Main Authors: Tallec, K., Paul-Pont, Ika, Petton, Bruno, Alunno-Bruscia, Marianne, Bourdon, C., Bernardini, I., Boulais, M., Lambert, Christophe, Quere, C., Bideau, Antoine, Le Goïc, Nelly, Cassone, A.-L., Le Grand, Fabienne, Fabioux, Caroline, Soudant, Philippe, Huvet, Arnaud
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Universita degli Studi di Padova, Department of Physical Sciences, Earth and Environment Siena (DSFTA), Università degli Studi di Siena = University of Siena (UNISI), This study was supported by the Agence Nationale de la Recherche (ANR) – Nanoplastics project (ANR-15-CE34-0006)., ANR-15-CE34-0006,Nanoplastics,Microplastiques, nanoplastiques dans l'environnement marin: caractérisation, impacts et évaluation des risques sanitaires.(2015)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2021
• Subjects: ACL; Oyster; panorama; CNRS; Ifremer; UBO; nanoplastics; embryonic exposure; larval performances; offspring; envir; geo; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://doi.org/10.1080/17435390.2021.1879963; https://hal.archives-ouvertes.fr/hal-03246774/file/Tallec%20et%20al.%202021.pdf; https://hal.archives-ouvertes.fr/hal-03246774

WOS:000616240600001 International audience Early life stages (ELS) of numerous marine invertebrates mustcope with man-made contaminants, including plastic debris, during their pelagic phase. Among the diversity of plastic particles, nano-sized debris, known as nanoplastics, can induce effects with severe outcomes in ELS of various biological models, including the Pacific oyster Crassostrea gigas. Here, we investigated the effects of a sub-lethal dose (0.1 mu g mL(-1)) of 50 nm polystyrene nanobeads (nano-PS) with amine functions on oyster embryos (24 h exposure) and we assessed consequences on larval and adult performances over two generations of oysters. Only a few effects were observed. Lipid analyses revealed that first-generation (G1) embryos exposed to nano-PS displayed a relative increase in cardiolipin content (+9.7%), suggesting a potential modification of mitochondrial functioning. G1-larvae issued from exposed embryos showed decreases in larval growth (-9%) and lipid storage (-20%). No effect was observed at the G1 adult stage in terms of growth, ecophysiological parameters (clearance and respiration rates, absorption efficiency), or reproductive outputs (gonadic development, gamete quality). Second generation (G2) larvae issued from control G1 displayed a significant growth reduction after G2 embryonic exposure to nano-PS (-24%) compared to control (as observed at the first generation), while no intergenerational effect was detected on G2 larvae issued from G1 exposed embryos. Overall, the present experimental study suggests a low incidence of a short embryonic exposure to nano-PS on oyster phenotypes along the entire life cycle until the next larval generation.