Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas )
Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a po...
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Online Access: | https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://archimer.ifremer.fr/doc/00643/75522/ |
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fttriple:oai:gotriple.eu:10670/1.18j855 2023-05-15T15:58:53+02:00 Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud 2020-01-01 https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://archimer.ifremer.fr/doc/00643/75522/ en eng Informa UK Limited doi:10.1080/17435390.2020.1808104 10670/1.18j855 https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://archimer.ifremer.fr/doc/00643/75522/ other Archimer, archive institutionnelle de l'Ifremer Nanotoxicology (1743-5390) (Informa UK Limited), 2020-09 , Vol. 14 , N. 8 , P. 1039-1057 envir socio Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ 2020 fttriple https://doi.org/10.1080/17435390.2020.1808104 2023-01-22T16:57:01Z Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL−1) of 50-nm polystyrene beads with amine (50-NH2 beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH2 beads at 10 µg mL−1 induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL−1 and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. Text Crassostrea gigas Unknown Nanotoxicology 14 8 1039 1057 |
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envir socio |
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envir socio Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
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envir socio |
description |
Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL−1) of 50-nm polystyrene beads with amine (50-NH2 beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH2 beads at 10 µg mL−1 induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL−1 and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. |
format |
Text |
author |
Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_facet |
Tallec, Kevin Paul-pont, Ika Boulais, Myrina Le Goïc, Nelly Gonzalez Fernandez, C. Le Grand, Fabienne Bideau, Antoine Quéré, Claudie Cassone, A.-l. Lambert, Christophe Soudant, Philippe Huvet, Arnaud |
author_sort |
Tallec, Kevin |
title |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_short |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_full |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_fullStr |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_full_unstemmed |
Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas ) |
title_sort |
nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( crassostrea gigas ) |
publisher |
Informa UK Limited |
publishDate |
2020 |
url |
https://doi.org/10.1080/17435390.2020.1808104 https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://archimer.ifremer.fr/doc/00643/75522/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Archimer, archive institutionnelle de l'Ifremer Nanotoxicology (1743-5390) (Informa UK Limited), 2020-09 , Vol. 14 , N. 8 , P. 1039-1057 |
op_relation |
doi:10.1080/17435390.2020.1808104 10670/1.18j855 https://archimer.ifremer.fr/doc/00643/75522/78210.pdf https://archimer.ifremer.fr/doc/00643/75522/ |
op_rights |
other |
op_doi |
https://doi.org/10.1080/17435390.2020.1808104 |
container_title |
Nanotoxicology |
container_volume |
14 |
container_issue |
8 |
container_start_page |
1039 |
op_container_end_page |
1057 |
_version_ |
1766394649839140864 |