Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V....
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Online Access: | https://doi.org/10.1186/s13567-017-0438-1 https://archimer.ifremer.fr/doc/00386/49768/50308.pdf https://archimer.ifremer.fr/doc/00386/49768/ |
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fttriple:oai:gotriple.eu:10670/1.0tzdxh 2023-05-15T15:58:42+02:00 Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas Travers, Marie-agnes Tourbiez, Delphine Parizadeh, Leila Haffner, Philippe Kozic-djellouli, Angelique Aboubaker, Mohamed Koken, Marcel Degremont, Lionel Lupo, Coralie https://doi.org/10.1186/s13567-017-0438-1 https://archimer.ifremer.fr/doc/00386/49768/50308.pdf https://archimer.ifremer.fr/doc/00386/49768/ en eng Biomed Central Ltd doi:10.1186/s13567-017-0438-1 10670/1.0tzdxh https://archimer.ifremer.fr/doc/00386/49768/50308.pdf https://archimer.ifremer.fr/doc/00386/49768/ other Archimer, archive institutionnelle de l'Ifremer Veterinary Research (0928-4249) (Biomed Central Ltd), 2017-05 , Vol. 48 , N. 32 , P. 1-8 envir socio Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1186/s13567-017-0438-1 2023-01-22T18:39:13Z This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. Text Crassostrea gigas Pacific oyster Unknown Pacific Veterinary Research 48 1 |
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envir socio Travers, Marie-agnes Tourbiez, Delphine Parizadeh, Leila Haffner, Philippe Kozic-djellouli, Angelique Aboubaker, Mohamed Koken, Marcel Degremont, Lionel Lupo, Coralie Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
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envir socio |
description |
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. |
format |
Text |
author |
Travers, Marie-agnes Tourbiez, Delphine Parizadeh, Leila Haffner, Philippe Kozic-djellouli, Angelique Aboubaker, Mohamed Koken, Marcel Degremont, Lionel Lupo, Coralie |
author_facet |
Travers, Marie-agnes Tourbiez, Delphine Parizadeh, Leila Haffner, Philippe Kozic-djellouli, Angelique Aboubaker, Mohamed Koken, Marcel Degremont, Lionel Lupo, Coralie |
author_sort |
Travers, Marie-agnes |
title |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
several strains, one disease: experimental investigation of vibrio aestuarianus infection parameters in the pacific oyster, crassostrea gigas |
publisher |
Biomed Central Ltd |
url |
https://doi.org/10.1186/s13567-017-0438-1 https://archimer.ifremer.fr/doc/00386/49768/50308.pdf https://archimer.ifremer.fr/doc/00386/49768/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Veterinary Research (0928-4249) (Biomed Central Ltd), 2017-05 , Vol. 48 , N. 32 , P. 1-8 |
op_relation |
doi:10.1186/s13567-017-0438-1 10670/1.0tzdxh https://archimer.ifremer.fr/doc/00386/49768/50308.pdf https://archimer.ifremer.fr/doc/00386/49768/ |
op_rights |
other |
op_doi |
https://doi.org/10.1186/s13567-017-0438-1 |
container_title |
Veterinary Research |
container_volume |
48 |
container_issue |
1 |
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1766394469037375488 |