Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts
Degree: Master of Science Abstract: An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation...
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University of Alberta. Department of Chemistry.
2011
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fttriple:oai:gotriple.eu:10402/era.27404 2023-05-15T16:22:41+02:00 Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts Cooper, Victoria Irene Li, Liang (Chemistry) Wong, Charles (Chemistry) Li, Xing-Fang (Laboratory Medicine and Pathology) 2011-01-11 http://hdl.handle.net/10402/era.27404 en eng University of Alberta. Department of Chemistry. 10402/era.27404 http://hdl.handle.net/10402/era.27404 ERA : Education and Research Archive envir geo Thesis https://vocabularies.coar-repositories.org/resource_types/c_46ec/ 2011 fttriple 2023-01-22T17:08:52Z Degree: Master of Science Abstract: An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10 chiral analytes in less than 91 minutes, improving upon previous gas chromatography-based methods. Use of a pyrenyl-ethyl silica column in the first dimension enabled separation of all but two pairs of isobaric analytes. Limits of detection of 0.01 to 1.73 ng on-column were achieved. The precision and accuracy were within acceptable limits, but poor sensitivity was achieved for several meta-methylsulfonyl-substituted congeners. Despite this limitation, the method was successfully applied to the analysis of Greenland sledge dog (Canis familiaris) plasma and adipose tissue extracts. Concentration and enantiomer fraction data are presented. None of the target analytes were detected in Norwegian glaucous gull (Larus hyperboreus) plasma extracts. Thesis Glaucous Gull Greenland Larus hyperboreus Unknown Greenland |
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envir geo Cooper, Victoria Irene Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
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description |
Degree: Master of Science Abstract: An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10 chiral analytes in less than 91 minutes, improving upon previous gas chromatography-based methods. Use of a pyrenyl-ethyl silica column in the first dimension enabled separation of all but two pairs of isobaric analytes. Limits of detection of 0.01 to 1.73 ng on-column were achieved. The precision and accuracy were within acceptable limits, but poor sensitivity was achieved for several meta-methylsulfonyl-substituted congeners. Despite this limitation, the method was successfully applied to the analysis of Greenland sledge dog (Canis familiaris) plasma and adipose tissue extracts. Concentration and enantiomer fraction data are presented. None of the target analytes were detected in Norwegian glaucous gull (Larus hyperboreus) plasma extracts. |
author2 |
Li, Liang (Chemistry) Wong, Charles (Chemistry) Li, Xing-Fang (Laboratory Medicine and Pathology) |
format |
Thesis |
author |
Cooper, Victoria Irene |
author_facet |
Cooper, Victoria Irene |
author_sort |
Cooper, Victoria Irene |
title |
Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
title_short |
Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
title_full |
Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
title_fullStr |
Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
title_full_unstemmed |
Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
title_sort |
development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts |
publisher |
University of Alberta. Department of Chemistry. |
publishDate |
2011 |
url |
http://hdl.handle.net/10402/era.27404 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Glaucous Gull Greenland Larus hyperboreus |
genre_facet |
Glaucous Gull Greenland Larus hyperboreus |
op_source |
ERA : Education and Research Archive |
op_relation |
10402/era.27404 http://hdl.handle.net/10402/era.27404 |
_version_ |
1766010753985282048 |