High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production
Abstract The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil exploration may take place. The exposure of fish to petrogenic contaminants can normally be detected...
Published in: | Marine Environmental Research |
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Language: | English |
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2012
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Online Access: | http://hdl.handle.net/2262/63614 https://doi.org/10.1016/j.marenvres.2011.04.006 |
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fttrinitycoll:oai:tara.tcd.ie:2262/63614 2023-05-15T15:38:50+02:00 High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production 2012-06-02T00:52:30Z http://hdl.handle.net/2262/63614 https://doi.org/10.1016/j.marenvres.2011.04.006 en eng Elsevier 01411136 (ISSN) S0141-1136(11)00049-3 (PII) S0141-1136(11)00049-3 (publisherID) http://hdl.handle.net/2262/63614 Marine Environmental Research doi:10.1016/j.marenvres.2011.04.006 2011 12 months Atlantic hagfish Myxine glutinosa bile fluorescence PAH biomonitoring Barents Sea 2012 fttrinitycoll https://doi.org/10.1016/j.marenvres.2011.04.006 2020-02-16T13:53:43Z Abstract The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil exploration may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish (Myxine glutinosa) collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of Myxine contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed. correspondence: Corresponding author:, Magne O. Sydnes, International Research Institute of Stavanger, P.O. Box 8046, N-4068, Stavanger, Norway, Tel +47 51875566, Fax +47 51875540. (Sydnes, Magne O.) magne.olav.sydnes@iris.no (Sydnes, Magne O.) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Sundt, Rolf C.) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Beyer, Jonny) University of Stavanger - N-4036 Stavanger--> - NORWAY (Beyer, Jonny) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Vingen, Sjur) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Sydnes, Magne O.) NORWAY (Sydnes, Magne O.) NORWAY Received: 2011-02-11 Revised: 2011-04-19 Accepted: 2011-04-25 Other/Unknown Material Barents Sea The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) Barents Sea Norway Marine Environmental Research 71 5 369 374 |
institution |
Open Polar |
collection |
The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) |
op_collection_id |
fttrinitycoll |
language |
English |
topic |
Atlantic hagfish Myxine glutinosa bile fluorescence PAH biomonitoring Barents Sea |
spellingShingle |
Atlantic hagfish Myxine glutinosa bile fluorescence PAH biomonitoring Barents Sea High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
topic_facet |
Atlantic hagfish Myxine glutinosa bile fluorescence PAH biomonitoring Barents Sea |
description |
Abstract The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil exploration may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish (Myxine glutinosa) collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of Myxine contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed. correspondence: Corresponding author:, Magne O. Sydnes, International Research Institute of Stavanger, P.O. Box 8046, N-4068, Stavanger, Norway, Tel +47 51875566, Fax +47 51875540. (Sydnes, Magne O.) magne.olav.sydnes@iris.no (Sydnes, Magne O.) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Sundt, Rolf C.) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Beyer, Jonny) University of Stavanger - N-4036 Stavanger--> - NORWAY (Beyer, Jonny) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Vingen, Sjur) IRIS - International Research Institute of Stavanger - P.O. Box 8046--> , N-4068--> , Stavanger--> - NORWAY (Sydnes, Magne O.) NORWAY (Sydnes, Magne O.) NORWAY Received: 2011-02-11 Revised: 2011-04-19 Accepted: 2011-04-25 |
title |
High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
title_short |
High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
title_full |
High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
title_fullStr |
High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
title_full_unstemmed |
High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production |
title_sort |
high matrix interference affecting detection of pah metabolites in bile of atlantic hagfish (myxine glutinosa) used for biomonitoring of deep-water oil production |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/2262/63614 https://doi.org/10.1016/j.marenvres.2011.04.006 |
geographic |
Barents Sea Norway |
geographic_facet |
Barents Sea Norway |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_relation |
01411136 (ISSN) S0141-1136(11)00049-3 (PII) S0141-1136(11)00049-3 (publisherID) http://hdl.handle.net/2262/63614 Marine Environmental Research doi:10.1016/j.marenvres.2011.04.006 |
op_rights |
2011 12 months |
op_doi |
https://doi.org/10.1016/j.marenvres.2011.04.006 |
container_title |
Marine Environmental Research |
container_volume |
71 |
container_issue |
5 |
container_start_page |
369 |
op_container_end_page |
374 |
_version_ |
1766370211231956992 |