Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba
Abstract We isolated and tested six novel microsatellite loci in Sanderling (Calidris alba) from Greenland for paternity analyses. In addition, we tested 11 already published microsatellite markers which were originally developed for the congeneric species, the Pectoral Sandpiper (C. melanotos). All...
Published in: | Journal of Ornithology |
---|---|
Language: | English |
Published: |
Springer-Verlag
2012
|
Subjects: | |
Online Access: | http://hdl.handle.net/2262/62869 https://doi.org/10.1007/s10336-011-0681-6 |
id |
fttrinitycoll:oai:tara.tcd.ie:2262/62869 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) |
op_collection_id |
fttrinitycoll |
language |
English |
topic |
Microsatellite markers Parentage analysis Breeding system Population genetics |
spellingShingle |
Microsatellite markers Parentage analysis Breeding system Population genetics Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
topic_facet |
Microsatellite markers Parentage analysis Breeding system Population genetics |
description |
Abstract We isolated and tested six novel microsatellite loci in Sanderling (Calidris alba) from Greenland for paternity analyses. In addition, we tested 11 already published microsatellite markers which were originally developed for the congeneric species, the Pectoral Sandpiper (C. melanotos). All loci were polymorphic, but five of the cross-species loci were not scorable due to suboptimal amplification patterns. The 12 successful loci were tested on 87 individuals, yielding an average of 9.0 (range 4?19) alleles per locus and mean expected heterozygosity of 0.70. Because this dataset contained families, tests for Hardy?Weinberg equilibrium, linkage disequilibrium and probability of identity were done on a subset of the data containing 25 adults caught in the same year. The overall probability of identity was 1.0 ? 10?13. Only one locus displayed significant homozygote excess and all loci were unlinked. On the basis of female heterozygotes, all loci are assumed to be autosomal. Zusammenfassung Wir isolierten und testeten sechs neue Mikrosatelliten-Loci auf Eignung fur Verwandtschaftsanalysen beim gronlandischen Sanderling (Calidris alba). Zusatzlich dazu testeten wir 11 bereits publizierte Mikrosatellitenmarker, die ursprunglich fur den nahe verwandten Graubruststrandlaufer (C. melanotos) entwickelt worden waren. Alle Genorte waren polymorph, aber funf der artubergreifenden loci konnten wegen ungenugender Vervielfaltigungsmuster nicht ausgewertet werden. Die 12 verbliebenen Genorte wurden fur 87 Individuen getestet und ergaben einen Durchschnitt von 9,0 Allelen pro Genort (Bereich: 4 -19) sowie eine mittlere zu erwartende Heterozygositat von 0,70. Weil dieses Set Daten von Familien enthielt, wurden fur einen Teil des Sets mit Daten von funf adulten, im gleichen Jahr gefangenen Vogeln auch statistische Tests fur das Hardy?Weinberg-Aquilibrium, das Kopplungs-Ungleichgewicht, und die Identitats-Wahrscheinlichkeit durchgefuhrt. Insgesamt betrug die Identitats-Wahrscheinlichkeit 1,0*10-13. Nur ein einziger locus zeigte einen signifikanten Homozygoten-Uberschu?, und alle loci waren ungekoppelt. Aufgrund der weiblichen Heterozygoten wurde angenommen, dass alle loci autosomal waren. phone: +31-222-369300 (Luttikhuizen, Pieternella C.) pieternella.luttikhuizen@nioz.nl (Luttikhuizen, Pieternella C.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Luttikhuizen, Pieternella C.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Bol, Anneke) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Witte, Harry) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Bleijswijk, Judith) Department of Natural History, Royal Ontario Museum - 100 Queen?s Park Crescent - M5S 2C6 - Toronto - CANADA (Haddrath, Oliver) Department of Natural History, Royal Ontario Museum - 100 Queen?s Park Crescent - M5S 2C6 - Toronto - CANADA (Baker, Allan J.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Piersma, Theunis) Centre for Ecological and Evolutionary Studies (CEES), Animal Ecology Group, University of Groningen - P.O. Box 11103 - 9700 CC - Groningen - NETHERLANDS (Piersma, Theunis) Centre for Ecological and Evolutionary Studies (CEES), Animal Ecology Group, University of Groningen - P.O. Box 11103 - 9700 CC - Groningen - NETHERLANDS (Reneerkens, Jeroen) CANADA NETHERLANDS Registration: 2011-03-07 Received: 2010-09-24 Revised: 2010-12-21 Accepted: 2011-03-07 ePublished: 2011-03-30 |
title |
Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
title_short |
Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
title_full |
Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
title_fullStr |
Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
title_full_unstemmed |
Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba |
title_sort |
novel and cross-species microsatellite markers for parentage analysis in sanderling calidris alba |
publisher |
Springer-Verlag |
publishDate |
2012 |
url |
http://hdl.handle.net/2262/62869 https://doi.org/10.1007/s10336-011-0681-6 |
long_lat |
ENVELOPE(9.843,9.843,63.758,63.758) |
geographic |
Bol Canada Greenland |
geographic_facet |
Bol Canada Greenland |
genre |
Calidris alba Greenland Sanderling |
genre_facet |
Calidris alba Greenland Sanderling |
op_relation |
0021-8375 (pISSN) 1439-0361 (eISSN) 0021-8375 (ISSN) 10336 (JournalID) s10336-011-0681-6 (publisherID) 681 (ArticleID) http://hdl.handle.net/2262/62869 Journal of Ornithology 152 3 807 810 doi:10.1007/s10336-011-0681-6 |
op_rights |
Dt. Ornithologen-Gesellschaft e.V., 2011 12 months |
op_doi |
https://doi.org/10.1007/s10336-011-0681-6 |
container_title |
Journal of Ornithology |
container_volume |
152 |
container_issue |
3 |
container_start_page |
807 |
op_container_end_page |
810 |
_version_ |
1766383155853393920 |
spelling |
fttrinitycoll:oai:tara.tcd.ie:2262/62869 2023-05-15T15:48:09+02:00 Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba 2012-03-30T00:58:51Z http://hdl.handle.net/2262/62869 https://doi.org/10.1007/s10336-011-0681-6 en eng Springer-Verlag Berlin/Heidelberg 0021-8375 (pISSN) 1439-0361 (eISSN) 0021-8375 (ISSN) 10336 (JournalID) s10336-011-0681-6 (publisherID) 681 (ArticleID) http://hdl.handle.net/2262/62869 Journal of Ornithology 152 3 807 810 doi:10.1007/s10336-011-0681-6 Dt. Ornithologen-Gesellschaft e.V., 2011 12 months Microsatellite markers Parentage analysis Breeding system Population genetics 2012 fttrinitycoll https://doi.org/10.1007/s10336-011-0681-6 2020-02-16T13:53:33Z Abstract We isolated and tested six novel microsatellite loci in Sanderling (Calidris alba) from Greenland for paternity analyses. In addition, we tested 11 already published microsatellite markers which were originally developed for the congeneric species, the Pectoral Sandpiper (C. melanotos). All loci were polymorphic, but five of the cross-species loci were not scorable due to suboptimal amplification patterns. The 12 successful loci were tested on 87 individuals, yielding an average of 9.0 (range 4?19) alleles per locus and mean expected heterozygosity of 0.70. Because this dataset contained families, tests for Hardy?Weinberg equilibrium, linkage disequilibrium and probability of identity were done on a subset of the data containing 25 adults caught in the same year. The overall probability of identity was 1.0 ? 10?13. Only one locus displayed significant homozygote excess and all loci were unlinked. On the basis of female heterozygotes, all loci are assumed to be autosomal. Zusammenfassung Wir isolierten und testeten sechs neue Mikrosatelliten-Loci auf Eignung fur Verwandtschaftsanalysen beim gronlandischen Sanderling (Calidris alba). Zusatzlich dazu testeten wir 11 bereits publizierte Mikrosatellitenmarker, die ursprunglich fur den nahe verwandten Graubruststrandlaufer (C. melanotos) entwickelt worden waren. Alle Genorte waren polymorph, aber funf der artubergreifenden loci konnten wegen ungenugender Vervielfaltigungsmuster nicht ausgewertet werden. Die 12 verbliebenen Genorte wurden fur 87 Individuen getestet und ergaben einen Durchschnitt von 9,0 Allelen pro Genort (Bereich: 4 -19) sowie eine mittlere zu erwartende Heterozygositat von 0,70. Weil dieses Set Daten von Familien enthielt, wurden fur einen Teil des Sets mit Daten von funf adulten, im gleichen Jahr gefangenen Vogeln auch statistische Tests fur das Hardy?Weinberg-Aquilibrium, das Kopplungs-Ungleichgewicht, und die Identitats-Wahrscheinlichkeit durchgefuhrt. Insgesamt betrug die Identitats-Wahrscheinlichkeit 1,0*10-13. Nur ein einziger locus zeigte einen signifikanten Homozygoten-Uberschu?, und alle loci waren ungekoppelt. Aufgrund der weiblichen Heterozygoten wurde angenommen, dass alle loci autosomal waren. phone: +31-222-369300 (Luttikhuizen, Pieternella C.) pieternella.luttikhuizen@nioz.nl (Luttikhuizen, Pieternella C.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Luttikhuizen, Pieternella C.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Bol, Anneke) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Witte, Harry) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Bleijswijk, Judith) Department of Natural History, Royal Ontario Museum - 100 Queen?s Park Crescent - M5S 2C6 - Toronto - CANADA (Haddrath, Oliver) Department of Natural History, Royal Ontario Museum - 100 Queen?s Park Crescent - M5S 2C6 - Toronto - CANADA (Baker, Allan J.) Royal Netherlands Institute for Sea Research (NIOZ) - P.O. Box 59 - 1790 AB - Texel - NETHERLANDS (Piersma, Theunis) Centre for Ecological and Evolutionary Studies (CEES), Animal Ecology Group, University of Groningen - P.O. Box 11103 - 9700 CC - Groningen - NETHERLANDS (Piersma, Theunis) Centre for Ecological and Evolutionary Studies (CEES), Animal Ecology Group, University of Groningen - P.O. Box 11103 - 9700 CC - Groningen - NETHERLANDS (Reneerkens, Jeroen) CANADA NETHERLANDS Registration: 2011-03-07 Received: 2010-09-24 Revised: 2010-12-21 Accepted: 2011-03-07 ePublished: 2011-03-30 Other/Unknown Material Calidris alba Greenland Sanderling The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) Bol ENVELOPE(9.843,9.843,63.758,63.758) Canada Greenland Journal of Ornithology 152 3 807 810 |