Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica)
Abstract Benthic diatoms represent an important element of global nutritional productivity; to raise attention on their role, which is often neglected due to analytical difficulties, surface (1 cm top layer) coastal sediments from Gerlache inlet to Penguin Bay at Terra Nova Bay were collected and st...
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Language: | English |
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2012
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Online Access: | http://hdl.handle.net/2262/62842 https://doi.org/10.1016/j.marenvres.2011.02.005 |
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fttrinitycoll:oai:tara.tcd.ie:2262/62842 2023-05-15T14:04:40+02:00 Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) 2012-03-30T00:53:44Z http://hdl.handle.net/2262/62842 https://doi.org/10.1016/j.marenvres.2011.02.005 en eng Elsevier 01411136 (ISSN) S0141-1136(11)00032-8 (PII) S0141-1136(11)00032-8 (publisherID) http://hdl.handle.net/2262/62842 Marine Environmental Research 71 4 304 doi:10.1016/j.marenvres.2011.02.005 2011 12 months diatoms real-time PCR coastal sediments SEM-EDX analyses salinity brines Terra Nova Bay 2012 fttrinitycoll https://doi.org/10.1016/j.marenvres.2011.02.005 2020-02-16T13:53:33Z Abstract Benthic diatoms represent an important element of global nutritional productivity; to raise attention on their role, which is often neglected due to analytical difficulties, surface (1 cm top layer) coastal sediments from Gerlache inlet to Penguin Bay at Terra Nova Bay were collected and stored at -20?C. DNA amplification by real-time-PCR, based on diatom-specific oligonucleotide primers designed on small-subunit rRNA (SSU rRNA), was performed in addition to diatom conventional cell counting and spectrophotometric determination of photo-pigments. Moreover, cations and anions were determined in sediments with the aim to identify factors involved in the control of diatom abundance. Diatom distribution was found quite heterogeneous displaying significant differences from site to site. The salinity in sediments ranged from 45.1 at Gerlache inlet to 76.2 at Penguin Bay and it was correlated with cell abundance, biodiversity, amount of pigments and amplified DNA. The dominant species, Fragilariopsis curta, was associated to sediment salinity brines. correspondence: Correspondence: Franco Baldi, Department of Environmental Science Ca Foscari University of Venezia, Tel. 041.2348901. (Baldi, Franco) baldi@unive.it (Baldi, Franco) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Baldi, Franco) ITALY (Baldi, Franco) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Facca, Chiara) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Marchetto, Davide) Laboratory of Molecular Biology and Microbial Biotechnologies, Department of Biosciences and Biotechnologies, University of Camerino - Via Gentile III da Varano; 62032 Camerino (MC) Italy--> - (Nguyen, Thi Nhu Mai) Laboratory of Molecular Biology and Microbial Biotechnologies, Department of Biosciences and Biotechnologies, University of Camerino - Via Gentile III da Varano; 62032 Camerino (MC) Italy--> - (Spurio, Roberto) ITALY Received: 2010-11-30 Revised: 2011-02-15 Accepted: 2011-02-18 Other/Unknown Material Antarc* Antarctica The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) Gentile ENVELOPE(-62.233,-62.233,-63.333,-63.333) Gerlache ENVELOPE(99.033,99.033,-66.500,-66.500) Gerlache Inlet ENVELOPE(164.100,164.100,-74.683,-74.683) Larga ENVELOPE(-60.767,-60.767,-62.467,-62.467) Penguin Bay ENVELOPE(-36.567,-36.567,-54.250,-54.250) Terra Nova Bay Marine Environmental Research 71 4 304 311 |
institution |
Open Polar |
collection |
The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) |
op_collection_id |
fttrinitycoll |
language |
English |
topic |
diatoms real-time PCR coastal sediments SEM-EDX analyses salinity brines Terra Nova Bay |
spellingShingle |
diatoms real-time PCR coastal sediments SEM-EDX analyses salinity brines Terra Nova Bay Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
topic_facet |
diatoms real-time PCR coastal sediments SEM-EDX analyses salinity brines Terra Nova Bay |
description |
Abstract Benthic diatoms represent an important element of global nutritional productivity; to raise attention on their role, which is often neglected due to analytical difficulties, surface (1 cm top layer) coastal sediments from Gerlache inlet to Penguin Bay at Terra Nova Bay were collected and stored at -20?C. DNA amplification by real-time-PCR, based on diatom-specific oligonucleotide primers designed on small-subunit rRNA (SSU rRNA), was performed in addition to diatom conventional cell counting and spectrophotometric determination of photo-pigments. Moreover, cations and anions were determined in sediments with the aim to identify factors involved in the control of diatom abundance. Diatom distribution was found quite heterogeneous displaying significant differences from site to site. The salinity in sediments ranged from 45.1 at Gerlache inlet to 76.2 at Penguin Bay and it was correlated with cell abundance, biodiversity, amount of pigments and amplified DNA. The dominant species, Fragilariopsis curta, was associated to sediment salinity brines. correspondence: Correspondence: Franco Baldi, Department of Environmental Science Ca Foscari University of Venezia, Tel. 041.2348901. (Baldi, Franco) baldi@unive.it (Baldi, Franco) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Baldi, Franco) ITALY (Baldi, Franco) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Facca, Chiara) Department of Environmental Sciences, Ca? Foscari University - Calle Larga S. Marta Dorsoduro 2137--> , 30123 Venezia--> - ITALY (Marchetto, Davide) Laboratory of Molecular Biology and Microbial Biotechnologies, Department of Biosciences and Biotechnologies, University of Camerino - Via Gentile III da Varano; 62032 Camerino (MC) Italy--> - (Nguyen, Thi Nhu Mai) Laboratory of Molecular Biology and Microbial Biotechnologies, Department of Biosciences and Biotechnologies, University of Camerino - Via Gentile III da Varano; 62032 Camerino (MC) Italy--> - (Spurio, Roberto) ITALY Received: 2010-11-30 Revised: 2011-02-15 Accepted: 2011-02-18 |
title |
Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
title_short |
Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
title_full |
Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
title_fullStr |
Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
title_full_unstemmed |
Diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
title_sort |
diatom quantification and their distribution with salinity brines in coastal sediments of terra nova bay (antarctica) |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/2262/62842 https://doi.org/10.1016/j.marenvres.2011.02.005 |
long_lat |
ENVELOPE(-62.233,-62.233,-63.333,-63.333) ENVELOPE(99.033,99.033,-66.500,-66.500) ENVELOPE(164.100,164.100,-74.683,-74.683) ENVELOPE(-60.767,-60.767,-62.467,-62.467) ENVELOPE(-36.567,-36.567,-54.250,-54.250) |
geographic |
Gentile Gerlache Gerlache Inlet Larga Penguin Bay Terra Nova Bay |
geographic_facet |
Gentile Gerlache Gerlache Inlet Larga Penguin Bay Terra Nova Bay |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
01411136 (ISSN) S0141-1136(11)00032-8 (PII) S0141-1136(11)00032-8 (publisherID) http://hdl.handle.net/2262/62842 Marine Environmental Research 71 4 304 doi:10.1016/j.marenvres.2011.02.005 |
op_rights |
2011 12 months |
op_doi |
https://doi.org/10.1016/j.marenvres.2011.02.005 |
container_title |
Marine Environmental Research |
container_volume |
71 |
container_issue |
4 |
container_start_page |
304 |
op_container_end_page |
311 |
_version_ |
1766275885827096576 |