Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions
Abstract Adhesion of pathogens to host tissues is the requirement for the initiation of the majority of infectious diseases. We recently showed that the binding of Neisseria meningitidis pili to immobilised human epithelial cells is inhibited by molecular size fractions (10?100 kDa) of berry juices....
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fttrinitycoll:oai:tara.tcd.ie:2262/56734 2023-05-15T15:59:27+02:00 Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions 2011-06-11T01:11:36Z http://hdl.handle.net/2262/56734 https://doi.org/10.1002/ptr.3349 en eng Wiley-Blackwell 0951-418X (pISSN) 1099-1573 (eISSN) 0951418X (ISSN) PTR-10-0307 (PII) PTR-10-0307.R1 (manuscript) http://hdl.handle.net/2262/56734 Phytotherapy Research doi:10.1002/ptr.3349 PTR (abbrev) 6 months Life Sciences 2011 fttrinitycoll https://doi.org/10.1002/ptr.3349 2020-02-16T13:52:19Z Abstract Adhesion of pathogens to host tissues is the requirement for the initiation of the majority of infectious diseases. We recently showed that the binding of Neisseria meningitidis pili to immobilised human epithelial cells is inhibited by molecular size fractions (10?100 kDa) of berry juices. Additionally, the isolated meningococcal pili bound to polyphenolic fractions of berry juices. In the present study we investigated the antiadhesive effects of berry juice polyphenolics against living meningococcal bacteria in a human epithelial cell culture model. The ability of bilberry, cranberry, crowberry, and lingonberry juice polyphenolic fractions to inhibit the attachment of N. meningitidis bacteria to HEC-1B human epithelial cells in a cell culture model was examined. Antibacterial effect of the fractions was tested using microtiter broth microdilution assay. The most effective adhesion inhibition of 75 % was achieved with cranberry juice polyphenolic fraction followed by crowberry (63%), bilberry (63%), and lingonberry (57%) juice polyphenolic fractions. Bacterial survival rates after incubation with the fractions varied between 75?100 %. The present results suggest berry juice polyphenols as inhibitors of adherence of N. meningitidis. Thus the binding of meningococci to berry juice polyphenols might be protective for the host against the infection. marko.toivanen@uku.fi (Toivanen, Marko) sanna.huttunen@uef.fi (Huttunen, Sanna) seppo.lapinjoki@uef.fi (Lapinjoki, Seppo) carina.tikkanen-kaukanen@uef.fi (Tikkanen-Kaukanen, Carina) University of Eastern Finland, School of Pharmacy - Kuopio - FINLAND (Toivanen, Marko) University of Eastern Finland, Institute of Public Health and Clinical Nutrition - P.O.Box 1627--> - FI-70211 - Kuopio - FINLAND (Huttunen, Sanna) University of Eastern Finland, School of Pharmacy - Kuopio - FINLAND (Lapinjoki, Seppo) University of Eastern Finland, Institute of Public Health and Clinical Nutrition - PO Box 1627--> - 70211 - Kuopio - FINLAND (Tikkanen-Kaukanen, Carina) FINLAND Other/Unknown Material Crowberry The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) Marko ENVELOPE(-60.750,-60.750,-62.467,-62.467) Sanna ENVELOPE(12.047,12.047,66.506,66.506) Phytotherapy Research 25 6 828 832 |
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Open Polar |
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The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) |
op_collection_id |
fttrinitycoll |
language |
English |
topic |
Life Sciences |
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Life Sciences Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
topic_facet |
Life Sciences |
description |
Abstract Adhesion of pathogens to host tissues is the requirement for the initiation of the majority of infectious diseases. We recently showed that the binding of Neisseria meningitidis pili to immobilised human epithelial cells is inhibited by molecular size fractions (10?100 kDa) of berry juices. Additionally, the isolated meningococcal pili bound to polyphenolic fractions of berry juices. In the present study we investigated the antiadhesive effects of berry juice polyphenolics against living meningococcal bacteria in a human epithelial cell culture model. The ability of bilberry, cranberry, crowberry, and lingonberry juice polyphenolic fractions to inhibit the attachment of N. meningitidis bacteria to HEC-1B human epithelial cells in a cell culture model was examined. Antibacterial effect of the fractions was tested using microtiter broth microdilution assay. The most effective adhesion inhibition of 75 % was achieved with cranberry juice polyphenolic fraction followed by crowberry (63%), bilberry (63%), and lingonberry (57%) juice polyphenolic fractions. Bacterial survival rates after incubation with the fractions varied between 75?100 %. The present results suggest berry juice polyphenols as inhibitors of adherence of N. meningitidis. Thus the binding of meningococci to berry juice polyphenols might be protective for the host against the infection. marko.toivanen@uku.fi (Toivanen, Marko) sanna.huttunen@uef.fi (Huttunen, Sanna) seppo.lapinjoki@uef.fi (Lapinjoki, Seppo) carina.tikkanen-kaukanen@uef.fi (Tikkanen-Kaukanen, Carina) University of Eastern Finland, School of Pharmacy - Kuopio - FINLAND (Toivanen, Marko) University of Eastern Finland, Institute of Public Health and Clinical Nutrition - P.O.Box 1627--> - FI-70211 - Kuopio - FINLAND (Huttunen, Sanna) University of Eastern Finland, School of Pharmacy - Kuopio - FINLAND (Lapinjoki, Seppo) University of Eastern Finland, Institute of Public Health and Clinical Nutrition - PO Box 1627--> - 70211 - Kuopio - FINLAND (Tikkanen-Kaukanen, Carina) FINLAND |
title |
Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
title_short |
Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
title_full |
Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
title_fullStr |
Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
title_full_unstemmed |
Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
title_sort |
inhibition of adhesion of neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions |
publisher |
Wiley-Blackwell |
publishDate |
2011 |
url |
http://hdl.handle.net/2262/56734 https://doi.org/10.1002/ptr.3349 |
long_lat |
ENVELOPE(-60.750,-60.750,-62.467,-62.467) ENVELOPE(12.047,12.047,66.506,66.506) |
geographic |
Marko Sanna |
geographic_facet |
Marko Sanna |
genre |
Crowberry |
genre_facet |
Crowberry |
op_relation |
0951-418X (pISSN) 1099-1573 (eISSN) 0951418X (ISSN) PTR-10-0307 (PII) PTR-10-0307.R1 (manuscript) http://hdl.handle.net/2262/56734 Phytotherapy Research doi:10.1002/ptr.3349 PTR (abbrev) |
op_rights |
6 months |
op_doi |
https://doi.org/10.1002/ptr.3349 |
container_title |
Phytotherapy Research |
container_volume |
25 |
container_issue |
6 |
container_start_page |
828 |
op_container_end_page |
832 |
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1766395404905086976 |