Esterification of phenolic acids catalyzed by lipases immobilized in organogels
Abstract Lipases from Rhizomucor miehei and Candida antarctica B were immobilized in hydroxypropylmethyl cellulose organogels based on surfactant-free microemulsions consisting of n-hexane, 1-propanol and water. Both lipases kept their catalytic activity, catalyzing the esterification reactions of v...
Published in: | Biotechnology Letters |
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Online Access: | http://hdl.handle.net/2262/55931 https://doi.org/10.1007/s10529-010-0305-x |
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fttrinitycoll:oai:tara.tcd.ie:2262/55931 2023-05-15T14:04:40+02:00 Esterification of phenolic acids catalyzed by lipases immobilized in organogels 2011-05-21T01:02:00Z http://hdl.handle.net/2262/55931 https://doi.org/10.1007/s10529-010-0305-x en eng Springer Netherlands Dordrecht 0141-5492 (pISSN) 1573-6776 (eISSN) 0141-5492 (ISSN) 10529 (JournalID) s10529-010-0305-x (publisherID) 305 (ArticleID) http://hdl.handle.net/2262/55931 Biotechnology Letters 32 10 1457 1462 doi:10.1007/s10529-010-0305-x Springer Science+Business Media B.V., 2010 12 months Biocatalysis Antioxidants Hydroxypropylmethyl cellulose 2011 fttrinitycoll https://doi.org/10.1007/s10529-010-0305-x 2020-02-16T13:52:12Z Abstract Lipases from Rhizomucor miehei and Candida antarctica B were immobilized in hydroxypropylmethyl cellulose organogels based on surfactant-free microemulsions consisting of n-hexane, 1-propanol and water. Both lipases kept their catalytic activity, catalyzing the esterification reactions of various phenolic acids including cinnamic acid derivatives. High reaction rates and yields (up to 94%) were obtained when lipase from C. antarctica was used. Kinetic studies have been performed and apparent kinetic constants were determined showing that ester synthesis catalyzed by immobilized lipases occurs via the Michaelis?Menten mechanism. phone: +302107273762 (Xenakis, A.) arisx@eie.gr (Xenakis, A.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Zoumpanioti, M.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Merianou, E.) Biological Applications and Technologies Department, University of Ioannina - Ioannina - GREECE (Merianou, E.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Karandreas, T.) Biological Applications and Technologies Department, University of Ioannina - Ioannina - GREECE (Stamatis, H.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Xenakis, A.) GREECE Registration: 2010-05-11 Received: 2010-03-10 Accepted: 2010-05-10 ePublished: 2010-05-21 Other/Unknown Material Antarc* Antarctica The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) Biotechnology Letters 32 10 1457 1462 |
institution |
Open Polar |
collection |
The University of Dublin, Trinity College: TARA (Trinity's Access to Research Archive) |
op_collection_id |
fttrinitycoll |
language |
English |
topic |
Biocatalysis Antioxidants Hydroxypropylmethyl cellulose |
spellingShingle |
Biocatalysis Antioxidants Hydroxypropylmethyl cellulose Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
topic_facet |
Biocatalysis Antioxidants Hydroxypropylmethyl cellulose |
description |
Abstract Lipases from Rhizomucor miehei and Candida antarctica B were immobilized in hydroxypropylmethyl cellulose organogels based on surfactant-free microemulsions consisting of n-hexane, 1-propanol and water. Both lipases kept their catalytic activity, catalyzing the esterification reactions of various phenolic acids including cinnamic acid derivatives. High reaction rates and yields (up to 94%) were obtained when lipase from C. antarctica was used. Kinetic studies have been performed and apparent kinetic constants were determined showing that ester synthesis catalyzed by immobilized lipases occurs via the Michaelis?Menten mechanism. phone: +302107273762 (Xenakis, A.) arisx@eie.gr (Xenakis, A.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Zoumpanioti, M.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Merianou, E.) Biological Applications and Technologies Department, University of Ioannina - Ioannina - GREECE (Merianou, E.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Karandreas, T.) Biological Applications and Technologies Department, University of Ioannina - Ioannina - GREECE (Stamatis, H.) Institute of Biological Research and Biotechnology, National Hellenic Research Foundation - Athens - GREECE (Xenakis, A.) GREECE Registration: 2010-05-11 Received: 2010-03-10 Accepted: 2010-05-10 ePublished: 2010-05-21 |
title |
Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
title_short |
Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
title_full |
Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
title_fullStr |
Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
title_full_unstemmed |
Esterification of phenolic acids catalyzed by lipases immobilized in organogels |
title_sort |
esterification of phenolic acids catalyzed by lipases immobilized in organogels |
publisher |
Springer Netherlands |
publishDate |
2011 |
url |
http://hdl.handle.net/2262/55931 https://doi.org/10.1007/s10529-010-0305-x |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
0141-5492 (pISSN) 1573-6776 (eISSN) 0141-5492 (ISSN) 10529 (JournalID) s10529-010-0305-x (publisherID) 305 (ArticleID) http://hdl.handle.net/2262/55931 Biotechnology Letters 32 10 1457 1462 doi:10.1007/s10529-010-0305-x |
op_rights |
Springer Science+Business Media B.V., 2010 12 months |
op_doi |
https://doi.org/10.1007/s10529-010-0305-x |
container_title |
Biotechnology Letters |
container_volume |
32 |
container_issue |
10 |
container_start_page |
1457 |
op_container_end_page |
1462 |
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1766275884001525760 |