Kinetics of hepatic phase I and II biotransformation reactions in 8 finfish species

• Published in: Marine Environmental Research
• Language: English
• Published: Elsevier 2011
• Subjects: hepatic; phase I and II biotransformation reactions; finfish; Gonzalez; Kane; Atlantic salmon; Salmo salar
• Online Access: http://hdl.handle.net/2262/50280; https://doi.org/10.1016/j.marenvres.2009.01.002

Abstract Hepatic microsomes and cytosols of channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar), red tilapia (Oreochromis sp.), largemouth bass (Micropterus salmoides), striped bass (Morone saxatilis), hybrid striped bass (Morone saxatilis x Morone crysops) , and bluegill (Lepomis macrochuris) (n = 8) were used to study the kinetics of phase I (ECOD, EROD, PROD, BROD) and phase II (UDP-glucuronosyltransferase (UDPGT)-, sulfotransferase (ST)- and glutathione-s-transferase (GST)- mediated) reactions. The best catalytic efficiency for ECOD and GST activities was performed by channel catfish, Atlantic salmon, rainbow trout and tilapia. The highest EROD catalytic efficiency was for Atlantic salmon. None of the species had either PROD or BROD activities. Rainbow trout had very similar UDPGT catalytic efficiency to tilapia, channel catfish, Atlantic salmon, largemouth bass and bluegill. Sulfotransferase conjugation had no significant differences among the species. In summary, tilapia, channel catfish, Atlantic salmon and rainbow trout had the best biotranforming capabilities; striped bass, hybrid striped bass and bluegill were low metabolizers and largemouth bass shared some capabilities with both groups. correspondence: Corresponding author. (Gonzalez M., Jaime Fernando) [e-mail] (Gonzalez M., Jaime Fernando) School of Veterinary Medicine & Animal Science - Universidad Nacional de Colombia - Bogota - Colombia--> - (Gonzalez M., Jaime Fernando) COLOMBIA (Gonzalez M., Jaime Fernando) Center for Veterinary Medicine ? Food Drug Administration (USFDA) - Laurel (MD) ? USA--> - (Reimschuessel, Renate) Center for Veterinary Medicine ? Food Drug Administration (USFDA) - Laurel (MD) ? USA--> - (Shaikh, Badar) College of Public Health & Health Professions ? University of Florida - Gainesville (FA) ? USA--> - (Kane, Andrew S.) COLOMBIA Received: 2008-10-13 Revised: 2009-01-05 Accepted: 2009-01-11