Evidence of recombination in Tula virus strains from Serbia
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions a...
Published in: | Infection, Genetics and Evolution |
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2014
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fttorlakinst:oai:intor.torlakinstitut.com:123456789/414 2023-05-15T17:12:34+02:00 Evidence of recombination in Tula virus strains from Serbia Nikolić, Valentina Stajković, Novica Stamenković, Gorana Čekanac, Radovan Marušić, Predrag Siljić, Marina Gligić, Ana Stanojević, Maja 2014 http://intor.torlakinstitut.com/handle/123456789/414 https://doi.org/10.1016/j.meegid.2013.08.020 unknown Elsevier, Amsterdam info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175024/RS// 1567-1348 http://intor.torlakinstitut.com/handle/123456789/414 doi:10.1016/j.meegid.2013.08.020 24008094 2-s2.0-84893774623 000332273800056 restrictedAccess ARR Infection Genetics and Evolution Recombination Tula virus Phylogenetic analysis Molecular evolution article publishedVersion 2014 fttorlakinst https://doi.org/10.1016/j.meegid.2013.08.020 2023-01-18T14:38:50Z Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Microtus arvalis InTOR - Repository of the Institute of Virology, Vaccines and Sera „Torlak” Tula ENVELOPE(-65.650,-65.650,-65.517,-65.517) Infection, Genetics and Evolution 21 472 478 |
institution |
Open Polar |
collection |
InTOR - Repository of the Institute of Virology, Vaccines and Sera „Torlak” |
op_collection_id |
fttorlakinst |
language |
unknown |
topic |
Recombination Tula virus Phylogenetic analysis Molecular evolution |
spellingShingle |
Recombination Tula virus Phylogenetic analysis Molecular evolution Nikolić, Valentina Stajković, Novica Stamenković, Gorana Čekanac, Radovan Marušić, Predrag Siljić, Marina Gligić, Ana Stanojević, Maja Evidence of recombination in Tula virus strains from Serbia |
topic_facet |
Recombination Tula virus Phylogenetic analysis Molecular evolution |
description |
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Nikolić, Valentina Stajković, Novica Stamenković, Gorana Čekanac, Radovan Marušić, Predrag Siljić, Marina Gligić, Ana Stanojević, Maja |
author_facet |
Nikolić, Valentina Stajković, Novica Stamenković, Gorana Čekanac, Radovan Marušić, Predrag Siljić, Marina Gligić, Ana Stanojević, Maja |
author_sort |
Nikolić, Valentina |
title |
Evidence of recombination in Tula virus strains from Serbia |
title_short |
Evidence of recombination in Tula virus strains from Serbia |
title_full |
Evidence of recombination in Tula virus strains from Serbia |
title_fullStr |
Evidence of recombination in Tula virus strains from Serbia |
title_full_unstemmed |
Evidence of recombination in Tula virus strains from Serbia |
title_sort |
evidence of recombination in tula virus strains from serbia |
publisher |
Elsevier, Amsterdam |
publishDate |
2014 |
url |
http://intor.torlakinstitut.com/handle/123456789/414 https://doi.org/10.1016/j.meegid.2013.08.020 |
long_lat |
ENVELOPE(-65.650,-65.650,-65.517,-65.517) |
geographic |
Tula |
geographic_facet |
Tula |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Infection Genetics and Evolution |
op_relation |
info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175024/RS// 1567-1348 http://intor.torlakinstitut.com/handle/123456789/414 doi:10.1016/j.meegid.2013.08.020 24008094 2-s2.0-84893774623 000332273800056 |
op_rights |
restrictedAccess ARR |
op_doi |
https://doi.org/10.1016/j.meegid.2013.08.020 |
container_title |
Infection, Genetics and Evolution |
container_volume |
21 |
container_start_page |
472 |
op_container_end_page |
478 |
_version_ |
1766069357375389696 |