Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile
Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation....
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fttomskstateuniv:koha:000925495 2023-05-15T16:37:18+02:00 Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile Aksenov, Andrey S. Shirokova, Liudmila S. Kisil, Oksana Ya. Kolesova, Sofiia N. Lim, Artem G. Kuzmina, Daria M. Pouillé, Sophie Alexis, Marie A. Castrec-Rouelle, Maryse Loiko, Sergey V. Pokrovsky, Oleg S. 2021 application/pdf https://doi.org/10.3390/d13070328 https://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000925495 eng eng koha:000925495 doi:10.3390/d13070328 https://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000925495 Diversity. 2021. Vol. 13, № 7. P. 328 (1-27) вечная мерзлота торф грунтовые воды бактерии метаболизм генетическое разнообразие Западная Сибирь статьи в журналах info:eu-repo/semantics/article 2021 fttomskstateuniv https://doi.org/10.3390/d13070328 2022-12-20T17:34:31Z Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobac-teria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microor-ganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two orders of magnitude ... Article in Journal/Newspaper Ice permafrost Siberia вечная мерзлота Tomsk State University Research Library Diversity 13 7 328 |
institution |
Open Polar |
collection |
Tomsk State University Research Library |
op_collection_id |
fttomskstateuniv |
language |
English |
topic |
вечная мерзлота торф грунтовые воды бактерии метаболизм генетическое разнообразие Западная Сибирь |
spellingShingle |
вечная мерзлота торф грунтовые воды бактерии метаболизм генетическое разнообразие Западная Сибирь Aksenov, Andrey S. Shirokova, Liudmila S. Kisil, Oksana Ya. Kolesova, Sofiia N. Lim, Artem G. Kuzmina, Daria M. Pouillé, Sophie Alexis, Marie A. Castrec-Rouelle, Maryse Loiko, Sergey V. Pokrovsky, Oleg S. Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
topic_facet |
вечная мерзлота торф грунтовые воды бактерии метаболизм генетическое разнообразие Западная Сибирь |
description |
Permafrost peatlands, containing a sizable amount of soil organic carbon (OC), play a pivotal role in soil (peat) OC transformation into soluble and volatile forms and greatly contribute to overall natural CO2 and CH4 emissions to the atmosphere under ongoing permafrost thaw and soil OC degradation. Peat microorganisms are largely responsible for the processing of this OC, yet coupled studies of chemical and bacterial parameters in permafrost peatlands are rather limited and geographically biased. Towards testing the possible impact of peat and peat pore water chemical composition on microbial population and diversity, here we present results of a preliminary study of the western Siberia permafrost peatland discontinuous permafrost zone. The quantitative evaluation of microorganisms and determination of microbial diversity along a 100 cm thick peat soil column, which included thawed and frozen peat and bottom mineral horizon, was performed by RT-PCR and 16S rRNA gene-based metagenomic analysis, respectively. Bacteria (mainly Proteobac-teria, Acidobacteria, Actinobacteria) strongly dominated the microbial diversity (99% sequences), with a negligible proportion of archaea (0.3–0.5%). There was a systematic evolution of main taxa according to depth, with a maximum of 65% (Acidobacteria) encountered in the active layer, or permafrost boundary (50–60 cm). We also measured C, N, nutrients and ~50 major and trace elements in peat (19 samples) as well as its pore water and dispersed ice (10 samples), sampled over the same core, and we analyzed organic matter quality in six organic and one mineral horizon of this core. Using multiparametric statistics (PCA), we tested the links between the total microbial number and 16S rRNA diversity and chemical composition of both the solid and fluid phase harboring the microor-ganisms. Under climate warming and permafrost thaw, one can expect a downward movement of the layer of maximal genetic diversity following the active layer thickening. Given a one to two orders of magnitude ... |
format |
Article in Journal/Newspaper |
author |
Aksenov, Andrey S. Shirokova, Liudmila S. Kisil, Oksana Ya. Kolesova, Sofiia N. Lim, Artem G. Kuzmina, Daria M. Pouillé, Sophie Alexis, Marie A. Castrec-Rouelle, Maryse Loiko, Sergey V. Pokrovsky, Oleg S. |
author_facet |
Aksenov, Andrey S. Shirokova, Liudmila S. Kisil, Oksana Ya. Kolesova, Sofiia N. Lim, Artem G. Kuzmina, Daria M. Pouillé, Sophie Alexis, Marie A. Castrec-Rouelle, Maryse Loiko, Sergey V. Pokrovsky, Oleg S. |
author_sort |
Aksenov, Andrey S. |
title |
Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
title_short |
Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
title_full |
Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
title_fullStr |
Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
title_full_unstemmed |
Bacterial number and genetic diversity in a permafrost peatland (Western Siberia): Testing a link with organic matter quality and elementary composition of a peat soil profile |
title_sort |
bacterial number and genetic diversity in a permafrost peatland (western siberia): testing a link with organic matter quality and elementary composition of a peat soil profile |
publishDate |
2021 |
url |
https://doi.org/10.3390/d13070328 https://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000925495 |
genre |
Ice permafrost Siberia вечная мерзлота |
genre_facet |
Ice permafrost Siberia вечная мерзлота |
op_source |
Diversity. 2021. Vol. 13, № 7. P. 328 (1-27) |
op_relation |
koha:000925495 doi:10.3390/d13070328 https://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000925495 |
op_doi |
https://doi.org/10.3390/d13070328 |
container_title |
Diversity |
container_volume |
13 |
container_issue |
7 |
container_start_page |
328 |
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1766027594232233984 |