Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species
Combining germ cell cryopreservation with transplantation is a powerful tool for preserving the genetic resources of various fish strains. In this germ cell transplantation system, since only type A spermatogonia (ASGs) among the various testicular cells have the potency to colonize recipient gonads...
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Elsevier B.V.
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fttokyounivmst:oai:oacis.repo.nii.ac.jp:00002320 2023-07-23T04:18:21+02:00 Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species Ichida Kensuke Matsushita Yoshiyuki Amano Yuichi Miwa Misako Nagasawa Kazue Hayashi Makoto Mizutani Hanaka Takahashi Momo Boonanuntanasarn Surintorn Yoshizaki Goro 2021-02-25 https://oacis.repo.nii.ac.jp/?action=repository_uri&item_id=2320 http://id.nii.ac.jp/1342/00002262/ en eng Elsevier B.V. https://doi.org/10.1016/j.aquaculture.2020.736096 https://oacis.repo.nii.ac.jp/?action=repository_uri&item_id=2320 http://id.nii.ac.jp/1342/00002262/ Aquaculture, 533, 736096(2021-02-25) 0044-8486 (c) 2020 Elsevier B.V. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.aquaculture.2020.736096 https://www.sciencedirect.com/science/article/am/pii/S004484862030199X https://www.kaiyodai.ac.jp/Japanese/IRBAS/index.html https://www.elsevier.com/ja-jp Cell surface antigen Monoclonal antibody Spermatogonia Brown trout Atlantic salmon Journal Article 2021 fttokyounivmst https://doi.org/10.1016/j.aquaculture.2020.736096 2023-07-01T19:49:40Z Combining germ cell cryopreservation with transplantation is a powerful tool for preserving the genetic resources of various fish strains. In this germ cell transplantation system, since only type A spermatogonia (ASGs) among the various testicular cells have the potency to colonize recipient gonads and resume gametogenesis, techniques for visualization, isolation, and tracing of ASGs facilitate ease of manipulation. Here we established an ASG visualization technique in two Salmo species: brown trout and Atlantic salmon. We applied a monoclonal antibody (antibody No. 95), which specifically recognizes a cell surface antigen of ASGs obtained from rainbow trout, against Salmo species. Immunohistochemistry studies showed that antibody No. 95 also specifically detected the ASGs of brown trout and Atlantic salmon. Furthermore, marker-gene analyses of brown trout testicular cells sorted by flow cytometry using Alexa flour 488-conjugated antibody No. 95 revealed that fluorescent-positive cells possessed molecular characteristics of ASGs. Finally, to investigate the transplantability of antibody-positive cells, ASGs pre-stained with antibody No. 95 were transplanted into the peritoneal cavity of rainbow trout larvae. As a result, fluorescent cells incorporated into the recipient gonads, suggesting that the visualized ASGs possess transplantability. Thus, it is expected that the ASG visualization methods developed in this study will facilitate the development of more efficiency of germ cell transplantation techniques in Salmo species. 公開日: 2020-12-26 Article in Journal/Newspaper Atlantic salmon TUMSAT-OACIS (Tokyo University of Marine Science and Technology: Open Access Collection of International and Scholarly Papers) Aquaculture 533 736096 |
institution |
Open Polar |
collection |
TUMSAT-OACIS (Tokyo University of Marine Science and Technology: Open Access Collection of International and Scholarly Papers) |
op_collection_id |
fttokyounivmst |
language |
English |
topic |
Cell surface antigen Monoclonal antibody Spermatogonia Brown trout Atlantic salmon |
spellingShingle |
Cell surface antigen Monoclonal antibody Spermatogonia Brown trout Atlantic salmon Ichida Kensuke Matsushita Yoshiyuki Amano Yuichi Miwa Misako Nagasawa Kazue Hayashi Makoto Mizutani Hanaka Takahashi Momo Boonanuntanasarn Surintorn Yoshizaki Goro Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
topic_facet |
Cell surface antigen Monoclonal antibody Spermatogonia Brown trout Atlantic salmon |
description |
Combining germ cell cryopreservation with transplantation is a powerful tool for preserving the genetic resources of various fish strains. In this germ cell transplantation system, since only type A spermatogonia (ASGs) among the various testicular cells have the potency to colonize recipient gonads and resume gametogenesis, techniques for visualization, isolation, and tracing of ASGs facilitate ease of manipulation. Here we established an ASG visualization technique in two Salmo species: brown trout and Atlantic salmon. We applied a monoclonal antibody (antibody No. 95), which specifically recognizes a cell surface antigen of ASGs obtained from rainbow trout, against Salmo species. Immunohistochemistry studies showed that antibody No. 95 also specifically detected the ASGs of brown trout and Atlantic salmon. Furthermore, marker-gene analyses of brown trout testicular cells sorted by flow cytometry using Alexa flour 488-conjugated antibody No. 95 revealed that fluorescent-positive cells possessed molecular characteristics of ASGs. Finally, to investigate the transplantability of antibody-positive cells, ASGs pre-stained with antibody No. 95 were transplanted into the peritoneal cavity of rainbow trout larvae. As a result, fluorescent cells incorporated into the recipient gonads, suggesting that the visualized ASGs possess transplantability. Thus, it is expected that the ASG visualization methods developed in this study will facilitate the development of more efficiency of germ cell transplantation techniques in Salmo species. 公開日: 2020-12-26 |
format |
Article in Journal/Newspaper |
author |
Ichida Kensuke Matsushita Yoshiyuki Amano Yuichi Miwa Misako Nagasawa Kazue Hayashi Makoto Mizutani Hanaka Takahashi Momo Boonanuntanasarn Surintorn Yoshizaki Goro |
author_facet |
Ichida Kensuke Matsushita Yoshiyuki Amano Yuichi Miwa Misako Nagasawa Kazue Hayashi Makoto Mizutani Hanaka Takahashi Momo Boonanuntanasarn Surintorn Yoshizaki Goro |
author_sort |
Ichida Kensuke |
title |
Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
title_short |
Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
title_full |
Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
title_fullStr |
Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
title_full_unstemmed |
Visualization and tracking of live type A spermatogonia using a fluorescence-conjugated antibody in Salmo species |
title_sort |
visualization and tracking of live type a spermatogonia using a fluorescence-conjugated antibody in salmo species |
publisher |
Elsevier B.V. |
publishDate |
2021 |
url |
https://oacis.repo.nii.ac.jp/?action=repository_uri&item_id=2320 http://id.nii.ac.jp/1342/00002262/ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
https://www.sciencedirect.com/science/article/am/pii/S004484862030199X https://www.kaiyodai.ac.jp/Japanese/IRBAS/index.html https://www.elsevier.com/ja-jp |
op_relation |
https://doi.org/10.1016/j.aquaculture.2020.736096 https://oacis.repo.nii.ac.jp/?action=repository_uri&item_id=2320 http://id.nii.ac.jp/1342/00002262/ Aquaculture, 533, 736096(2021-02-25) 0044-8486 |
op_rights |
(c) 2020 Elsevier B.V. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.aquaculture.2020.736096 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2020.736096 |
container_title |
Aquaculture |
container_volume |
533 |
container_start_page |
736096 |
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1772180621523681280 |