Gynogenetic Tetraploid Larvae of the Pacific Oyster Crassostrea gigas Induced by Inhibition of the 1st and 2nd Meiotic Divisions

• Main Authors: LI Qi, KIJIMA Akihiro
• Format: Report
• Language: English
• Published: Faculty of Agriculture, Tohoku University 2006
• Subjects: Pacific; Crassostrea gigas; Pacific oyster
• Online Access: http://hdl.handle.net/10097/40396; https://tohoku.repo.nii.ac.jp/?action=repository_uri&item_id=65519; https://tohoku.repo.nii.ac.jp/?action=repository_action_common_download&item_id=65519&item_no=1&attribute_id=18&file_no=1

The induction of gynogenetic tetraploids by the suppression of the meiotic division in the Pacific oyster Crassostrea gigas was attempted in three trials using the treatments of cytochalasin B (0.5μg/mL; CB). The effects of various durations of CB treatment on tetraploid induction frequency were examined. Haploid gynogenesis was induced by sperm which were UV-irradiated for 60s at an intensity of 72erg/mm^2/s. CB treatment was effective in inhibiting meiosis, and the optimum time for the suppression of both the 1st and 2nd polar bodies was from 10 to 45min postinsemination, yielding 51.4% gynogenetic tetraploids. The results of this study suggest the possibility of producing gynogenetic tetraploid Pacific oysters.