Production of Phenolic Compounds in Cell Suspensions of Arctic Bramble and Cloudberry

• Main Author: Viitanen, Arto
• Other Authors: Metropolia Ammattikorkeakoulu
• Format: Bachelor Thesis
• Language: English
• Published: Metropolia Ammattikorkeakoulu 2015
• Subjects: UPLC-MS; cloudberry; arctic bramble; phenolic compounds; Biolääketiede; fi=Bio- ja elintarviketekniikka|sv=Bio- och livsmedelsteknik|en=Biotechnology; Food Industry|; Bio- ja elintarviketekniikka; Arctic; Rubus chamaemorus
• Online Access: http://www.theseus.fi/handle/10024/93456

Phenolic compounds are of interest for various industries, and can be found in differing qualities and quantities between plant species, with particularly high concentrations observed in Nordic berries. The natural yields of these berries are highly vulnerable to environmental conditions, and consequently methods for producing these valuable compounds in bioreactors are being investigated. However, the phenolic content in all cultures is highly dependent on growth conditions and the used cell line, and the synthesis pathways leading to these compounds are poorly understood. Although the profiles of phenolic contents for certain berry species have been well characterized in literature, the profiles for cell suspension lines are largely unknown. Cell suspension cultures have significant advantages for production of phenolic compounds over traditional agricultural methods, and more research on the cell suspensions’ production capabilities and a better understanding of the biosynthesis pathways is needed before utilization in the industries can be achieved. In this thesis, basic research was made on several arctic bramble (Rubus arcticus) and cloudberry (Rubus chamaemorus) cell suspension lines regarding their growth characteristics, profiles of phenolic compounds, and modification of the synthesis pathways. The pathways were stimulated with two elicitors, methyl jasmonate and ethephon. The phenolic compounds were analysed by UPLC-MS during different stages of the growth cycle and the elicitations, to display representative changes in the profiles of the different cell lines over time. The obtained results show substantial variations in the growth rates and the profiles of phenolic compounds even within cell suspension lines of the same species. Both elicitors were found to increase production of phenolic compounds, and one unidentified novel phenolic compound was found to be synthesized by stimulation with ethephon in an arctic bramble cell suspension line. Of the two species, arctic bramble was invariably found to ...