Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus
Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves...
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fttexasamuniv:oai:repository.tamu.edu:1969.1/ETD-TAMU-2002-THESIS-W28 2023-05-15T18:06:00+02:00 Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus Webb, Kenneth Ashley 2002 electronic application/pdf reformatted digital http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W28 en_US eng Texas A&M University http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W28 This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. nutrition Major nutrition Thesis text 2002 fttexasamuniv 2015-02-07T23:24:07Z Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves 35-40). Issued also on microfiche from Lange Micrographics. The red drum (Sciaenops ocellatus) has traditionally been an important commercial and recreational fish species in the Gulf of Mexico; therefore, its aquacultural production for food and for stock enhancement continues to develop. The minimum dietary lysine requirement of juvenile red drum was previously quantified to be 1.55% of a 35% crude protein (CP) diet (4.4% of dietary protein). However, red drum are usually fed diets containing 40 to 50% CP under commercial production. Therefore, the purpose of this study was to reevaluate the dietary lysine requirement of red drum as a function of dietary CP, and determine the effects of dietary manipulations on ammonia excretion. Control diets at 35 and 45% CP contained only the intact protein provided by a 50/50 mixture of red drum muscle and wheat gluten. Four experimental diets at each CP level contained the mixture (64% of CP) and crystalline amino acids (34% of CP) to provide lysine levels above and below the previously determined requirement. Each diet was fed to triplicate groups of 20 juvenile red drum initially averaging 3.4 g/fish in 110-l aquaria containing brackish (7ppt) water at 27?1 ?C and operated in a recirculating mode. Diets were fed at a fixed rate approaching apparent satiation twice daily for 6 weeks after which total ammonia nitrogen (TAN) excretion at 4-h postprandial was determined. Diets supplemented with crystalline amino acids supported similar weight gain (94 - 98%) as that obtained by fish fed control diets with intact protein. Based on weight gain, protein efficiency ratio (PER), and protein conversion efficiency (PCE) data, the minimum dietary lysine requirement was not influenced by dietary CP. Broken-line regression analysis of weight gain data of fish fed increments of lysine in both 35 and 45% CP diets yielded a lysine requirement estimate of 1.49?0.07% of diet, confirming the previously determined value. Weight gain and TAN excretion were significantly (P#0.05) higher in fish fed the 45% CP diets while PER and PCE values were significantly reduced. Lysine deficiency also resulted in elevated ammonia excretion, but significant reductions were not achieved when dietary lysine was at or above the established requirement. Thesis Red drum Sciaenops ocellatus Texas A&M University Digital Repository |
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Texas A&M University Digital Repository |
op_collection_id |
fttexasamuniv |
language |
English |
topic |
nutrition Major nutrition |
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nutrition Major nutrition Webb, Kenneth Ashley Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
topic_facet |
nutrition Major nutrition |
description |
Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves 35-40). Issued also on microfiche from Lange Micrographics. The red drum (Sciaenops ocellatus) has traditionally been an important commercial and recreational fish species in the Gulf of Mexico; therefore, its aquacultural production for food and for stock enhancement continues to develop. The minimum dietary lysine requirement of juvenile red drum was previously quantified to be 1.55% of a 35% crude protein (CP) diet (4.4% of dietary protein). However, red drum are usually fed diets containing 40 to 50% CP under commercial production. Therefore, the purpose of this study was to reevaluate the dietary lysine requirement of red drum as a function of dietary CP, and determine the effects of dietary manipulations on ammonia excretion. Control diets at 35 and 45% CP contained only the intact protein provided by a 50/50 mixture of red drum muscle and wheat gluten. Four experimental diets at each CP level contained the mixture (64% of CP) and crystalline amino acids (34% of CP) to provide lysine levels above and below the previously determined requirement. Each diet was fed to triplicate groups of 20 juvenile red drum initially averaging 3.4 g/fish in 110-l aquaria containing brackish (7ppt) water at 27?1 ?C and operated in a recirculating mode. Diets were fed at a fixed rate approaching apparent satiation twice daily for 6 weeks after which total ammonia nitrogen (TAN) excretion at 4-h postprandial was determined. Diets supplemented with crystalline amino acids supported similar weight gain (94 - 98%) as that obtained by fish fed control diets with intact protein. Based on weight gain, protein efficiency ratio (PER), and protein conversion efficiency (PCE) data, the minimum dietary lysine requirement was not influenced by dietary CP. Broken-line regression analysis of weight gain data of fish fed increments of lysine in both 35 and 45% CP diets yielded a lysine requirement estimate of 1.49?0.07% of diet, confirming the previously determined value. Weight gain and TAN excretion were significantly (P#0.05) higher in fish fed the 45% CP diets while PER and PCE values were significantly reduced. Lysine deficiency also resulted in elevated ammonia excretion, but significant reductions were not achieved when dietary lysine was at or above the established requirement. |
format |
Thesis |
author |
Webb, Kenneth Ashley |
author_facet |
Webb, Kenneth Ashley |
author_sort |
Webb, Kenneth Ashley |
title |
Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
title_short |
Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
title_full |
Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
title_fullStr |
Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
title_full_unstemmed |
Effects of lysine nutrition on production characteristics and ammonia excretion of red drum Sciaenops ocellatus |
title_sort |
effects of lysine nutrition on production characteristics and ammonia excretion of red drum sciaenops ocellatus |
publisher |
Texas A&M University |
publishDate |
2002 |
url |
http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W28 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W28 |
op_rights |
This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. |
_version_ |
1766177566050222080 |