Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia
Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves...
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fttexasamuniv:oai:repository.tamu.edu:1969.1/ETD-TAMU-2002-THESIS-F97 2023-05-15T16:33:04+02:00 Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia Fuson, Amanda Lynn 2002 electronic application/pdf reformatted digital http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-F97 en_US eng Texas A&M University http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-F97 This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. wildlife and fisheries sciences Major wildlife and fisheries sciences Thesis text 2002 fttexasamuniv 2015-02-07T23:23:51Z Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves 50-56). Issued also on microfiche from Lange Micrographics. Pinnipeds (seals and sea lions) have an elevated mitochondrial volume density [Vv(mt)] and citrate synthase (CS) and ?-hydroxyacyl-CoA dehydrogenase (HOAD) activity in their swimming muscles, to maintain aerobic metabolism, as an adaptation for diving hypoxia. However, little is known about the aerobic capacity of other organs, such as the heart, kidneys, and splanchnic organs. The goal of this study was to determine if any of these organs have an elevated Vv(mt) and CS and HOAD activity as an adaptation for sustaining aerobic metabolism and normal function during hypoxia. Samples of heart, liver, kidney, stomach, and small intestine were taken from ten freshly dead harbor seals (Phoca vitulina) and fixed in glutaraldehyde for transmission electron microscopy or frozen in liquid nitrogen for enzymatic analysis. Samples from dogs and rats were used for comparative purposes. Mitochondrial volume density per volume of tissue scaled to mass specific metabolic rate in the heart, liver, kidneys, stomach and small intestine of harbor seals was elevated when compared to that of terrestrial control animals such as dog and rat. The Vv(mt)/RMR (RMR = mass specific resting metabolic rate) of the harbor seal heart was 1.4 and 2.3X greater than the dog and rat, respectively. Vv(mt)/RMR of the harbor seal liver was 2.5 and 5X greater than the dog and rat, respectively. Vv(mt)/RMR of the harbor seal kidney was 2 and 4X greater than the dog and rat, respectively. The Vv(mt)/RMR of the harbor seal stomach was 1.8 and 4.5X greater than the dog and rat, respectively. The Vv(mt)/RMR of the harbor seal intestine was 3 and 3.3X greater than the dog and rat, respectively. CS and HOAD activity scaled to mass specific metabolic rate in the heart and liver of harbor seals was elevated over the dog and rat as well (2.3 and 1.4X in the heart, and 2.5 and 5X in the liver). These data suggest that organs such as the liver, kidneys, and stomach also possess a heightened ability for aerobic, fat-based metabolism during the hypoxia associated with routine diving. Thesis harbor seal Phoca vitulina Texas A&M University Digital Repository |
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Open Polar |
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Texas A&M University Digital Repository |
op_collection_id |
fttexasamuniv |
language |
English |
topic |
wildlife and fisheries sciences Major wildlife and fisheries sciences |
spellingShingle |
wildlife and fisheries sciences Major wildlife and fisheries sciences Fuson, Amanda Lynn Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
topic_facet |
wildlife and fisheries sciences Major wildlife and fisheries sciences |
description |
Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item. Includes bibliographical references (leaves 50-56). Issued also on microfiche from Lange Micrographics. Pinnipeds (seals and sea lions) have an elevated mitochondrial volume density [Vv(mt)] and citrate synthase (CS) and ?-hydroxyacyl-CoA dehydrogenase (HOAD) activity in their swimming muscles, to maintain aerobic metabolism, as an adaptation for diving hypoxia. However, little is known about the aerobic capacity of other organs, such as the heart, kidneys, and splanchnic organs. The goal of this study was to determine if any of these organs have an elevated Vv(mt) and CS and HOAD activity as an adaptation for sustaining aerobic metabolism and normal function during hypoxia. Samples of heart, liver, kidney, stomach, and small intestine were taken from ten freshly dead harbor seals (Phoca vitulina) and fixed in glutaraldehyde for transmission electron microscopy or frozen in liquid nitrogen for enzymatic analysis. Samples from dogs and rats were used for comparative purposes. Mitochondrial volume density per volume of tissue scaled to mass specific metabolic rate in the heart, liver, kidneys, stomach and small intestine of harbor seals was elevated when compared to that of terrestrial control animals such as dog and rat. The Vv(mt)/RMR (RMR = mass specific resting metabolic rate) of the harbor seal heart was 1.4 and 2.3X greater than the dog and rat, respectively. Vv(mt)/RMR of the harbor seal liver was 2.5 and 5X greater than the dog and rat, respectively. Vv(mt)/RMR of the harbor seal kidney was 2 and 4X greater than the dog and rat, respectively. The Vv(mt)/RMR of the harbor seal stomach was 1.8 and 4.5X greater than the dog and rat, respectively. The Vv(mt)/RMR of the harbor seal intestine was 3 and 3.3X greater than the dog and rat, respectively. CS and HOAD activity scaled to mass specific metabolic rate in the heart and liver of harbor seals was elevated over the dog and rat as well (2.3 and 1.4X in the heart, and 2.5 and 5X in the liver). These data suggest that organs such as the liver, kidneys, and stomach also possess a heightened ability for aerobic, fat-based metabolism during the hypoxia associated with routine diving. |
format |
Thesis |
author |
Fuson, Amanda Lynn |
author_facet |
Fuson, Amanda Lynn |
author_sort |
Fuson, Amanda Lynn |
title |
Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
title_short |
Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
title_full |
Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
title_fullStr |
Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
title_full_unstemmed |
Aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
title_sort |
aerobic capacities in the heart, kidneys, and splanchnic organs of harbor seals: adaptations to diving hypoxia |
publisher |
Texas A&M University |
publishDate |
2002 |
url |
http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-F97 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_relation |
http://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-F97 |
op_rights |
This thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use. |
_version_ |
1766022787009347584 |