Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus)
Thyrotropin (TSH) is a glycoprotein hormone released from the pituitary gland to promote the synthesis and secretion of thyroid hormone. The existence of well-established peripheral mechanisms for regulation of thyroid hormone delivery to targets has called into question the significance of TSH as a...
Main Author: | |
---|---|
Other Authors: | , , , |
Format: | Thesis |
Language: | English |
Published: |
2011
|
Subjects: | |
Online Access: | https://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9393 |
id |
fttexasamuniv:oai:oaktrust.library.tamu.edu:1969.1/ETD-TAMU-2011-05-9393 |
---|---|
record_format |
openpolar |
spelling |
fttexasamuniv:oai:oaktrust.library.tamu.edu:1969.1/ETD-TAMU-2011-05-9393 2023-07-16T04:00:41+02:00 Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) Miller, Thomas Charles MacKenzie, Duncan S. Jaques, Scott Gatlin, Delbert Rosenthal, Gil May 2011 application/pdf https://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9393 en_US eng https://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9393 thyrotropin teleost fish thyroid hormone goldfish red drum bioassay Thesis text 2011 fttexasamuniv 2023-06-27T22:22:10Z Thyrotropin (TSH) is a glycoprotein hormone released from the pituitary gland to promote the synthesis and secretion of thyroid hormone. The existence of well-established peripheral mechanisms for regulation of thyroid hormone delivery to targets has called into question the significance of TSH as a primary regulator of circulating thyroid hormone concentrations in fish. However, relatively little is known about the regulation or action of endogenously secreted teleost TSH, largely due to lack of purified TSH suitable for biological testing and immunoassay development. I developed a red drum in vivo bioassay to aid in the production and purification of recombinant TSH from the red drum, a perciform fish demonstrating dynamic daily thyroxine (T4) cycles hypothesized to be driven by TSH. Exogenous bovine TSH injection resulted in a time and dose-dependent increase in circulating TSH and T4 in red drum. However, the sensitivity of the red drum thyroid gland to stimulation by bovine TSH was lost during growth under controlled laboratory conditions, even when circulating levels of exogenously-administered mammalian TSH remained elevated. The insensitivity of the thyroid was not due to prior TSH injection or feed source. Because insensitivity of the Thyrotropin (TSH) is a glycoprotein hormone released from the pituitary gland to promote the synthesis and secretion of thyroid hormone. The existence of well-established peripheral mechanisms for regulation of thyroid hormone delivery to targets has called into question the significance of TSH as a primary regulator of circulating thyroid hormone concentrations in fish. However, relatively little is known about the regulation or action of endogenously secreted teleost TSH, largely due to lack of purified TSH suitable for biological testing and immunoassay development. I developed a red drum in vivo bioassay to aid in the production and purification of recombinant TSH from the red drum, a perciform fish demonstrating dynamic daily thyroxine (T4) cycles hypothesized to be ... Thesis Red drum Sciaenops ocellatus Texas A&M University Digital Repository |
institution |
Open Polar |
collection |
Texas A&M University Digital Repository |
op_collection_id |
fttexasamuniv |
language |
English |
topic |
thyrotropin teleost fish thyroid hormone goldfish red drum bioassay |
spellingShingle |
thyrotropin teleost fish thyroid hormone goldfish red drum bioassay Miller, Thomas Charles Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
topic_facet |
thyrotropin teleost fish thyroid hormone goldfish red drum bioassay |
description |
Thyrotropin (TSH) is a glycoprotein hormone released from the pituitary gland to promote the synthesis and secretion of thyroid hormone. The existence of well-established peripheral mechanisms for regulation of thyroid hormone delivery to targets has called into question the significance of TSH as a primary regulator of circulating thyroid hormone concentrations in fish. However, relatively little is known about the regulation or action of endogenously secreted teleost TSH, largely due to lack of purified TSH suitable for biological testing and immunoassay development. I developed a red drum in vivo bioassay to aid in the production and purification of recombinant TSH from the red drum, a perciform fish demonstrating dynamic daily thyroxine (T4) cycles hypothesized to be driven by TSH. Exogenous bovine TSH injection resulted in a time and dose-dependent increase in circulating TSH and T4 in red drum. However, the sensitivity of the red drum thyroid gland to stimulation by bovine TSH was lost during growth under controlled laboratory conditions, even when circulating levels of exogenously-administered mammalian TSH remained elevated. The insensitivity of the thyroid was not due to prior TSH injection or feed source. Because insensitivity of the Thyrotropin (TSH) is a glycoprotein hormone released from the pituitary gland to promote the synthesis and secretion of thyroid hormone. The existence of well-established peripheral mechanisms for regulation of thyroid hormone delivery to targets has called into question the significance of TSH as a primary regulator of circulating thyroid hormone concentrations in fish. However, relatively little is known about the regulation or action of endogenously secreted teleost TSH, largely due to lack of purified TSH suitable for biological testing and immunoassay development. I developed a red drum in vivo bioassay to aid in the production and purification of recombinant TSH from the red drum, a perciform fish demonstrating dynamic daily thyroxine (T4) cycles hypothesized to be ... |
author2 |
MacKenzie, Duncan S. Jaques, Scott Gatlin, Delbert Rosenthal, Gil |
format |
Thesis |
author |
Miller, Thomas Charles |
author_facet |
Miller, Thomas Charles |
author_sort |
Miller, Thomas Charles |
title |
Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
title_short |
Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
title_full |
Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
title_fullStr |
Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
title_full_unstemmed |
Biological Activity of Thyrotropin in Two Teleost Fish, Red Drum (Sciaenops ocellatus) and Goldfish (Carassius auratus) |
title_sort |
biological activity of thyrotropin in two teleost fish, red drum (sciaenops ocellatus) and goldfish (carassius auratus) |
publishDate |
2011 |
url |
https://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9393 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
https://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9393 |
_version_ |
1771549702592921600 |