Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio)
In all vertebrates thyroid hormones are essential for normal growth, metabolism, and development. Thyroid hormones are unusual in that they require iodine to function. While the pathway of iodide uptake in terrestrial vertebrates has been established to be strictly dietary, and is mediated by the sa...
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fttexasamuniv:oai:oaktrust.library.tamu.edu:1969.1/157674 2023-07-16T04:00:41+02:00 Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) Martillotti, Anthony William MacKenzie, Duncan S 2016-09-05T14:37:19Z application/pdf https://hdl.handle.net/1969.1/157674 unknown https://hdl.handle.net/1969.1/157674 Thyroid iodine red drum zebrafish Thesis text 2016 fttexasamuniv 2023-06-27T22:26:33Z In all vertebrates thyroid hormones are essential for normal growth, metabolism, and development. Thyroid hormones are unusual in that they require iodine to function. While the pathway of iodide uptake in terrestrial vertebrates has been established to be strictly dietary, and is mediated by the same sodium-iodide symporter (NIS) protein that concentrates iodine in the thyroid, the method of iodine uptake in fish remains controversial. In this study, I attempted to contrast the expression of a potential orthologue of NIS in two species of teleost fish, the red drum (Sciaenops ocellatus), a marine species living in an iodide rich environment, and the zebrafish (Danio rerio), a freshwater species living in an iodide poor environment. I applied RT-PCR to the digestive tract, the subpharyngeal region, and the gill, to determine whether fish in low iodine environments may possess a more active branchial iodide uptake pathway. I found that red drum held at higher salinities lack significant expression of nis in the gill but did not successfully identify the expression pattern of nis in zebrafish. Further research is needed to accomplish this goal. Thesis Red drum Sciaenops ocellatus Texas A&M University Digital Repository |
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Texas A&M University Digital Repository |
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Thyroid iodine red drum zebrafish |
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Thyroid iodine red drum zebrafish Martillotti, Anthony William Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
topic_facet |
Thyroid iodine red drum zebrafish |
description |
In all vertebrates thyroid hormones are essential for normal growth, metabolism, and development. Thyroid hormones are unusual in that they require iodine to function. While the pathway of iodide uptake in terrestrial vertebrates has been established to be strictly dietary, and is mediated by the same sodium-iodide symporter (NIS) protein that concentrates iodine in the thyroid, the method of iodine uptake in fish remains controversial. In this study, I attempted to contrast the expression of a potential orthologue of NIS in two species of teleost fish, the red drum (Sciaenops ocellatus), a marine species living in an iodide rich environment, and the zebrafish (Danio rerio), a freshwater species living in an iodide poor environment. I applied RT-PCR to the digestive tract, the subpharyngeal region, and the gill, to determine whether fish in low iodine environments may possess a more active branchial iodide uptake pathway. I found that red drum held at higher salinities lack significant expression of nis in the gill but did not successfully identify the expression pattern of nis in zebrafish. Further research is needed to accomplish this goal. |
author2 |
MacKenzie, Duncan S |
format |
Thesis |
author |
Martillotti, Anthony William |
author_facet |
Martillotti, Anthony William |
author_sort |
Martillotti, Anthony William |
title |
Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
title_short |
Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
title_full |
Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
title_fullStr |
Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
title_full_unstemmed |
Differential Expression of the Sodium-Iodide Symporter in Two Species of Teleost Fish, Red Drum (Sciaenops ocellatus) and Zebrafish (Danio rerio) |
title_sort |
differential expression of the sodium-iodide symporter in two species of teleost fish, red drum (sciaenops ocellatus) and zebrafish (danio rerio) |
publishDate |
2016 |
url |
https://hdl.handle.net/1969.1/157674 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
https://hdl.handle.net/1969.1/157674 |
_version_ |
1771549705850847232 |