Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR
Pancreas disease is responsible for major economic losses in the European salmonid farming industry, due to mortality, diminished growth rate and reduced slaughter weights. The infectious agent causing pancreas disease in Atlantic salmon was first isolated in Scotland in 1995 and later classified as...
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ftszentistvanufv:oai:www.huveta.hu:10832/1758 2023-06-06T11:52:01+02:00 Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR Kverneland, Elisabeth 2017 http://hdl.handle.net/10832/1758 en eng B-11561 http://hdl.handle.net/10832/1758 Thesis 2017 ftszentistvanufv 2023-04-13T18:54:02Z Pancreas disease is responsible for major economic losses in the European salmonid farming industry, due to mortality, diminished growth rate and reduced slaughter weights. The infectious agent causing pancreas disease in Atlantic salmon was first isolated in Scotland in 1995 and later classified as an Alphavirus, the first Alphavirus to be isolated that affects fish. One hallmark of Alphavirus infection in vertebrate cells is the ability of the pathogen to shut down host transcription and translation processes without affecting viral protein and nucleic acid synthesis. It is of interest to find out if SAV shares this characteristic. The goal of this project was to investigate Salmonid alphaviruses ability to down-regulate host cell transcription. Through using a two-step quantitative PCR for analysis of different housekeeping genes in non-infected and infected cells, we hoped to evaluate if SAV3 causes down-regulation of transcription. If so, at what time interval it occurred, and to what degree. This study and its incurred results served as a pilot study, to optimize and fine-tune the methodology for further experimentation. Thesis Atlantic salmon HuVetA - Hungarian Veterinary Archive Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
HuVetA - Hungarian Veterinary Archive |
op_collection_id |
ftszentistvanufv |
language |
English |
description |
Pancreas disease is responsible for major economic losses in the European salmonid farming industry, due to mortality, diminished growth rate and reduced slaughter weights. The infectious agent causing pancreas disease in Atlantic salmon was first isolated in Scotland in 1995 and later classified as an Alphavirus, the first Alphavirus to be isolated that affects fish. One hallmark of Alphavirus infection in vertebrate cells is the ability of the pathogen to shut down host transcription and translation processes without affecting viral protein and nucleic acid synthesis. It is of interest to find out if SAV shares this characteristic. The goal of this project was to investigate Salmonid alphaviruses ability to down-regulate host cell transcription. Through using a two-step quantitative PCR for analysis of different housekeeping genes in non-infected and infected cells, we hoped to evaluate if SAV3 causes down-regulation of transcription. If so, at what time interval it occurred, and to what degree. This study and its incurred results served as a pilot study, to optimize and fine-tune the methodology for further experimentation. |
format |
Thesis |
author |
Kverneland, Elisabeth |
spellingShingle |
Kverneland, Elisabeth Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
author_facet |
Kverneland, Elisabeth |
author_sort |
Kverneland, Elisabeth |
title |
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
title_short |
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
title_full |
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
title_fullStr |
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
title_full_unstemmed |
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR |
title_sort |
effect of salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time pcr |
publishDate |
2017 |
url |
http://hdl.handle.net/10832/1758 |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Slaughter Sav’ |
geographic_facet |
Slaughter Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
B-11561 http://hdl.handle.net/10832/1758 |
_version_ |
1767957853119709184 |