Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland
In East Greenland, a dramatic increase of tuberculosis (TB) incidence has been observed in recent years. Classical genotyping suggests a genetically similar Mycobacterium tuberculosis (Mtb) strain population as cause, however, precise transmission patterns are unclear. We performed whole genome sequ...
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ftsydanskunivpub:oai:sdu.dk:publications/d644e5e9-7325-40c9-a934-1ebb5d01e9cd 2024-09-09T19:38:41+00:00 Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland Bjorn-Mortensen, K Soborg, B Koch, A Ladefoged, K Merker, M Lillebaek, T Andersen, A B Niemann, S Kohl, T A 2016 application/pdf https://portal.findresearcher.sdu.dk/da/publications/d644e5e9-7325-40c9-a934-1ebb5d01e9cd https://doi.org/10.1038/srep33180 https://findresearcher.sdu.dk/ws/files/122953543/Tracing_Mycobacterium_tuberculosis_transmission_by_whole_genome_sequencing.pdf eng eng https://portal.findresearcher.sdu.dk/da/publications/d644e5e9-7325-40c9-a934-1ebb5d01e9cd info:eu-repo/semantics/openAccess Bjorn-Mortensen , K , Soborg , B , Koch , A , Ladefoged , K , Merker , M , Lillebaek , T , Andersen , A B , Niemann , S & Kohl , T A 2016 , ' Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting : a retrospective population-based study in East Greenland ' , Scientific Reports , vol. 6 , 33180 . https://doi.org/10.1038/srep33180 Journal Article article 2016 ftsydanskunivpub https://doi.org/10.1038/srep33180 2024-06-18T14:33:32Z In East Greenland, a dramatic increase of tuberculosis (TB) incidence has been observed in recent years. Classical genotyping suggests a genetically similar Mycobacterium tuberculosis (Mtb) strain population as cause, however, precise transmission patterns are unclear. We performed whole genome sequencing (WGS) of Mtb isolates from 98% of culture-positive TB cases through 21 years (n = 182) which revealed four genomic clusters of the Euro-American lineage (mainly sub-lineage 4.8 (n = 134)). The time to the most recent common ancestor of lineage 4.8 strains was found to be 100 years. This sub-lineage further diversified in the 1970s, and massively expanded in the 1990s, a period of lowered TB awareness in Greenland. Despite the low genetic strain diversity, WGS data revealed several recent short-term transmission events in line with the increasing incidence in the region. Thus, the isolated setting and the uniformity of circulating Mtb strains indicated that the majority of East Greenlandic TB cases originated from one or few strains introduced within the last century. Thereby, the study shows the consequences of even short interruptions in TB control efforts in previously TB high incidence areas and demonstrates the potential role of WGS in detecting ongoing micro epidemics, thus guiding public health efforts in the future. Article in Journal/Newspaper East Greenland Greenland greenlandic University of Southern Denmark Research Portal Greenland Scientific Reports 6 1 |
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Journal Article Bjorn-Mortensen, K Soborg, B Koch, A Ladefoged, K Merker, M Lillebaek, T Andersen, A B Niemann, S Kohl, T A Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
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Journal Article |
description |
In East Greenland, a dramatic increase of tuberculosis (TB) incidence has been observed in recent years. Classical genotyping suggests a genetically similar Mycobacterium tuberculosis (Mtb) strain population as cause, however, precise transmission patterns are unclear. We performed whole genome sequencing (WGS) of Mtb isolates from 98% of culture-positive TB cases through 21 years (n = 182) which revealed four genomic clusters of the Euro-American lineage (mainly sub-lineage 4.8 (n = 134)). The time to the most recent common ancestor of lineage 4.8 strains was found to be 100 years. This sub-lineage further diversified in the 1970s, and massively expanded in the 1990s, a period of lowered TB awareness in Greenland. Despite the low genetic strain diversity, WGS data revealed several recent short-term transmission events in line with the increasing incidence in the region. Thus, the isolated setting and the uniformity of circulating Mtb strains indicated that the majority of East Greenlandic TB cases originated from one or few strains introduced within the last century. Thereby, the study shows the consequences of even short interruptions in TB control efforts in previously TB high incidence areas and demonstrates the potential role of WGS in detecting ongoing micro epidemics, thus guiding public health efforts in the future. |
format |
Article in Journal/Newspaper |
author |
Bjorn-Mortensen, K Soborg, B Koch, A Ladefoged, K Merker, M Lillebaek, T Andersen, A B Niemann, S Kohl, T A |
author_facet |
Bjorn-Mortensen, K Soborg, B Koch, A Ladefoged, K Merker, M Lillebaek, T Andersen, A B Niemann, S Kohl, T A |
author_sort |
Bjorn-Mortensen, K |
title |
Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
title_short |
Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
title_full |
Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
title_fullStr |
Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
title_full_unstemmed |
Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in East Greenland |
title_sort |
tracing mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting:a retrospective population-based study in east greenland |
publishDate |
2016 |
url |
https://portal.findresearcher.sdu.dk/da/publications/d644e5e9-7325-40c9-a934-1ebb5d01e9cd https://doi.org/10.1038/srep33180 https://findresearcher.sdu.dk/ws/files/122953543/Tracing_Mycobacterium_tuberculosis_transmission_by_whole_genome_sequencing.pdf |
geographic |
Greenland |
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Greenland |
genre |
East Greenland Greenland greenlandic |
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East Greenland Greenland greenlandic |
op_source |
Bjorn-Mortensen , K , Soborg , B , Koch , A , Ladefoged , K , Merker , M , Lillebaek , T , Andersen , A B , Niemann , S & Kohl , T A 2016 , ' Tracing Mycobacterium tuberculosis transmission by whole genome sequencing in a high incidence setting : a retrospective population-based study in East Greenland ' , Scientific Reports , vol. 6 , 33180 . https://doi.org/10.1038/srep33180 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/d644e5e9-7325-40c9-a934-1ebb5d01e9cd |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/srep33180 |
container_title |
Scientific Reports |
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6 |
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1 |
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1809907574064021504 |