Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice

Sea ice is a biome of immense size and provides a range of habitats for diverse microbial communities, many of which are adapted to living at low temperatures and high salinities in brines. We measured simultaneous incorporation of thymidine (TdR) and leucine (Leu), bacterial cell abundance and cell...

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Published in:Aquatic Microbial Ecology
Main Authors: Kaartokallio, H., Sogaard, D. H., Norman, L., Rysgaard, S., Tison, J. L., Delille, B., Thomas, D. N.
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Online Access:https://portal.findresearcher.sdu.dk/da/publications/cdb9e483-b201-41b4-a296-10d9dc76ef04
https://doi.org/10.3354/ame01667
id ftsydanskunivpub:oai:sdu.dk:publications/cdb9e483-b201-41b4-a296-10d9dc76ef04
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spelling ftsydanskunivpub:oai:sdu.dk:publications/cdb9e483-b201-41b4-a296-10d9dc76ef04 2024-09-15T18:09:58+00:00 Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice Kaartokallio, H. Sogaard, D. H. Norman, L. Rysgaard, S. Tison, J. L. Delille, B. Thomas, D. N. 2013 https://portal.findresearcher.sdu.dk/da/publications/cdb9e483-b201-41b4-a296-10d9dc76ef04 https://doi.org/10.3354/ame01667 eng eng https://portal.findresearcher.sdu.dk/da/publications/cdb9e483-b201-41b4-a296-10d9dc76ef04 info:eu-repo/semantics/restrictedAccess Kaartokallio , H , Sogaard , D H , Norman , L , Rysgaard , S , Tison , J L , Delille , B & Thomas , D N 2013 , ' Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice ' , Aquatic Microbial Ecology , vol. 71 , no. 1 , pp. 57-73 . https://doi.org/10.3354/ame01667 Bacteria Flow cytometry Leucine Polyhydroxyalkanoates Sea ice Temporal variability Thymidine article 2013 ftsydanskunivpub https://doi.org/10.3354/ame01667 2024-08-19T23:51:22Z Sea ice is a biome of immense size and provides a range of habitats for diverse microbial communities, many of which are adapted to living at low temperatures and high salinities in brines. We measured simultaneous incorporation of thymidine (TdR) and leucine (Leu), bacterial cell abundance and cell population properties (by flow cytometry) in subarctic sea ice in SW Greenland. Short-term temporal variability was moderate, and steep environmental gradients, typical for sea ice, were the main drivers of the variability in bacterial cell properties and activity. Low nucleic acid (LNA) bacteria, previously linked to oligotrophic ecotypes in marine habitats, were more abundant in the upper ice layers, whereas high nucleic acid (HNA) bacteria dominated in lower ice, where organic carbon was in high concentrations. Leu incorporation was saturated at micromolar concentrations, as known from freshwater and marine biofilm systems. Leu: TdR ratios were high (up to >300) in lowermost ice layers, and when compared to published respiration measurements, these results suggest non-specific Leu incorporation. There was evidence of polyhydroxyalkanoate (PHA)-containing bacteria in the sea ice, shown by brightly fluorescing intracellular inclusions after Nile Blue A staining. High Leu saturating concentrations coupled with the occurrence of PHA-producing organisms further highlight the similarity of sea ice internal habitats to biofilm-like systems rather than to open-water systems. Article in Journal/Newspaper Greenland Sea ice Subarctic University of Southern Denmark Research Portal Aquatic Microbial Ecology 71 1 57 73
institution Open Polar
collection University of Southern Denmark Research Portal
op_collection_id ftsydanskunivpub
language English
topic Bacteria
Flow cytometry
Leucine
Polyhydroxyalkanoates
Sea ice
Temporal variability
Thymidine
spellingShingle Bacteria
Flow cytometry
Leucine
Polyhydroxyalkanoates
Sea ice
Temporal variability
Thymidine
Kaartokallio, H.
Sogaard, D. H.
Norman, L.
Rysgaard, S.
Tison, J. L.
Delille, B.
Thomas, D. N.
Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
topic_facet Bacteria
Flow cytometry
Leucine
Polyhydroxyalkanoates
Sea ice
Temporal variability
Thymidine
description Sea ice is a biome of immense size and provides a range of habitats for diverse microbial communities, many of which are adapted to living at low temperatures and high salinities in brines. We measured simultaneous incorporation of thymidine (TdR) and leucine (Leu), bacterial cell abundance and cell population properties (by flow cytometry) in subarctic sea ice in SW Greenland. Short-term temporal variability was moderate, and steep environmental gradients, typical for sea ice, were the main drivers of the variability in bacterial cell properties and activity. Low nucleic acid (LNA) bacteria, previously linked to oligotrophic ecotypes in marine habitats, were more abundant in the upper ice layers, whereas high nucleic acid (HNA) bacteria dominated in lower ice, where organic carbon was in high concentrations. Leu incorporation was saturated at micromolar concentrations, as known from freshwater and marine biofilm systems. Leu: TdR ratios were high (up to >300) in lowermost ice layers, and when compared to published respiration measurements, these results suggest non-specific Leu incorporation. There was evidence of polyhydroxyalkanoate (PHA)-containing bacteria in the sea ice, shown by brightly fluorescing intracellular inclusions after Nile Blue A staining. High Leu saturating concentrations coupled with the occurrence of PHA-producing organisms further highlight the similarity of sea ice internal habitats to biofilm-like systems rather than to open-water systems.
format Article in Journal/Newspaper
author Kaartokallio, H.
Sogaard, D. H.
Norman, L.
Rysgaard, S.
Tison, J. L.
Delille, B.
Thomas, D. N.
author_facet Kaartokallio, H.
Sogaard, D. H.
Norman, L.
Rysgaard, S.
Tison, J. L.
Delille, B.
Thomas, D. N.
author_sort Kaartokallio, H.
title Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
title_short Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
title_full Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
title_fullStr Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
title_full_unstemmed Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
title_sort short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice
publishDate 2013
url https://portal.findresearcher.sdu.dk/da/publications/cdb9e483-b201-41b4-a296-10d9dc76ef04
https://doi.org/10.3354/ame01667
genre Greenland
Sea ice
Subarctic
genre_facet Greenland
Sea ice
Subarctic
op_source Kaartokallio , H , Sogaard , D H , Norman , L , Rysgaard , S , Tison , J L , Delille , B & Thomas , D N 2013 , ' Short-term variability in bacterial abundance, cell properties, and incorporation of leucine and thymidine in subarctic sea ice ' , Aquatic Microbial Ecology , vol. 71 , no. 1 , pp. 57-73 . https://doi.org/10.3354/ame01667
op_relation https://portal.findresearcher.sdu.dk/da/publications/cdb9e483-b201-41b4-a296-10d9dc76ef04
op_rights info:eu-repo/semantics/restrictedAccess
op_doi https://doi.org/10.3354/ame01667
container_title Aquatic Microbial Ecology
container_volume 71
container_issue 1
container_start_page 57
op_container_end_page 73
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