Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface
Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water-sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous system for bio...
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/b2ee2450-1117-4eca-9062-2bc89c53d163 https://doi.org/10.1002/cssc.202002121 https://findresearcher.sdu.dk/ws/files/178327560/cssc.202002121.pdf |
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ftsydanskunivpub:oai:sdu.dk:publications/b2ee2450-1117-4eca-9062-2bc89c53d163 2023-05-15T14:02:27+02:00 Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface Sun, Zhiyong Cai, Meng Hübner, René Ansorge-Schumacher, Marion B. Wu, Changzhu 2020-12-17 application/pdf https://portal.findresearcher.sdu.dk/da/publications/b2ee2450-1117-4eca-9062-2bc89c53d163 https://doi.org/10.1002/cssc.202002121 https://findresearcher.sdu.dk/ws/files/178327560/cssc.202002121.pdf eng eng info:eu-repo/semantics/openAccess Sun , Z , Cai , M , Hübner , R , Ansorge-Schumacher , M B & Wu , C 2020 , ' Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface ' , ChemSusChem , vol. 13 , no. 24 , pp. 6523-6527 . https://doi.org/10.1002/cssc.202002121 biphasic biocatalysis enzyme catalysis nanoconjugates nonaqueous Pickering emulsions solvent-free reactions article 2020 ftsydanskunivpub https://doi.org/10.1002/cssc.202002121 2022-08-14T10:38:49Z Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water-sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous system for biotransformation. Herein, we present biocatalytically active nonaqueous PEs, stabilized by particle-enzyme nanoconjugates, for the fast transesterification and esterification, and eventually for biodiesel synthesis. Our nanoconjugates are the hybrid biocatalysts tailor-made by loading hydrophilic Candida antarctica lipase B onto hydrophobic silica nanoparticles, resulting in not only catalytically active but highly amphiphilic particles for stabilization of a methanol-decane emulsion. The enzyme activity in these PEs is significantly enhanced, ca. 375-fold higher than in the nonaqueous biphasic control. Moreover, the PEs can be multiply reused without significant loss of enzyme performance. With this proof-of-concept, this system can be expanded for many advanced syntheses using different enzymes in the future. Article in Journal/Newspaper Antarc* Antarctica University of Southern Denmark Research Portal ChemSusChem 13 24 6523 6527 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
topic |
biphasic biocatalysis enzyme catalysis nanoconjugates nonaqueous Pickering emulsions solvent-free reactions |
spellingShingle |
biphasic biocatalysis enzyme catalysis nanoconjugates nonaqueous Pickering emulsions solvent-free reactions Sun, Zhiyong Cai, Meng Hübner, René Ansorge-Schumacher, Marion B. Wu, Changzhu Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
topic_facet |
biphasic biocatalysis enzyme catalysis nanoconjugates nonaqueous Pickering emulsions solvent-free reactions |
description |
Nonaqueous Pickering emulsions (PEs) are a powerful platform for catalysis design, offering both a large interface contact and a preferable environment for water-sensitive synthesis. However, up to now, little progress has been made to incorporate insoluble enzymes into the nonaqueous system for biotransformation. Herein, we present biocatalytically active nonaqueous PEs, stabilized by particle-enzyme nanoconjugates, for the fast transesterification and esterification, and eventually for biodiesel synthesis. Our nanoconjugates are the hybrid biocatalysts tailor-made by loading hydrophilic Candida antarctica lipase B onto hydrophobic silica nanoparticles, resulting in not only catalytically active but highly amphiphilic particles for stabilization of a methanol-decane emulsion. The enzyme activity in these PEs is significantly enhanced, ca. 375-fold higher than in the nonaqueous biphasic control. Moreover, the PEs can be multiply reused without significant loss of enzyme performance. With this proof-of-concept, this system can be expanded for many advanced syntheses using different enzymes in the future. |
format |
Article in Journal/Newspaper |
author |
Sun, Zhiyong Cai, Meng Hübner, René Ansorge-Schumacher, Marion B. Wu, Changzhu |
author_facet |
Sun, Zhiyong Cai, Meng Hübner, René Ansorge-Schumacher, Marion B. Wu, Changzhu |
author_sort |
Sun, Zhiyong |
title |
Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
title_short |
Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
title_full |
Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
title_fullStr |
Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
title_full_unstemmed |
Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface |
title_sort |
tailoring particle-enzyme nanoconjugates for biocatalysis at the organic-organic interface |
publishDate |
2020 |
url |
https://portal.findresearcher.sdu.dk/da/publications/b2ee2450-1117-4eca-9062-2bc89c53d163 https://doi.org/10.1002/cssc.202002121 https://findresearcher.sdu.dk/ws/files/178327560/cssc.202002121.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Sun , Z , Cai , M , Hübner , R , Ansorge-Schumacher , M B & Wu , C 2020 , ' Tailoring Particle-Enzyme Nanoconjugates for Biocatalysis at the Organic-Organic Interface ' , ChemSusChem , vol. 13 , no. 24 , pp. 6523-6527 . https://doi.org/10.1002/cssc.202002121 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/cssc.202002121 |
container_title |
ChemSusChem |
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13 |
container_issue |
24 |
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6523 |
op_container_end_page |
6527 |
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1766272734973657088 |