Emergence of vanA Enterococcus faecium in Denmark, 2005-15
Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. M...
Published in: | Journal of Antimicrobial Chemotherapy |
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Format: | Article in Journal/Newspaper |
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2017
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb https://doi.org/10.1093/jac/dkx138 |
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ftsydanskunivpub:oai:sdu.dk:publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb 2024-09-15T18:05:41+00:00 Emergence of vanA Enterococcus faecium in Denmark, 2005-15 Hammerum, Anette M Baig, Sharmin Kamel, Yasmin Roer, Louise Pinholt, Mette Gumpert, Heidi Holzknecht, Barbara Juliane Røder, Bent Justesen, Ulrik S Samulioniené, Jurgita Kjærsgaard, Mona Østergaard, Claus Holm, Anette Dzajic, Esad Søndergaard, Turid Snekloth Gaini, Shahin Edquist, Petra Alm, Erik Lilje, Berit Westh, Henrik Stegger, Marc Hasman, Henrik 2017 https://portal.findresearcher.sdu.dk/da/publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb https://doi.org/10.1093/jac/dkx138 eng eng https://portal.findresearcher.sdu.dk/da/publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb info:eu-repo/semantics/closedAccess Hammerum , A M , Baig , S , Kamel , Y , Roer , L , Pinholt , M , Gumpert , H , Holzknecht , B J , Røder , B , Justesen , U S , Samulioniené , J , Kjærsgaard , M , Østergaard , C , Holm , A , Dzajic , E , Søndergaard , T S , Gaini , S , Edquist , P , Alm , E , Lilje , B , Westh , H , Stegger , M & Hasman , H 2017 , ' Emergence of vanA Enterococcus faecium in Denmark, 2005-15 ' , Journal of Antimicrobial Chemotherapy , vol. 72 , no. 8 , pp. 2184-2190 . https://doi.org/10.1093/jac/dkx138 Journal Article article 2017 ftsydanskunivpub https://doi.org/10.1093/jac/dkx138 2024-07-22T23:48:46Z Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. Methods: During 2005-14, all clinical VRE isolates were tested for the presence of vanA/B/C genes by PCR. In 2015, all clinical VRE isolates were whole-genome sequenced. From the WGS data, the presence of van genes and MLST STs were extracted in silico . Core-genome MLST (cgMLST) analysis was performed for the vancomycin-resistant E. faecium isolates. Results: During 2005-15, 1043 vanA E. faecium , 25 vanB E. faecium , 4 vanA E. faecalis and 28 vanB E. faecalis were detected. The number of VRE was <50 isolates/year until 2012 to > 200 isolates/year in 2013-15. In 2015, 368 vanA E. faecium and 1 vanB E. faecium were detected along with 1 vanA E. faecalis and 1 vanB E. faecalis . cgMLST subdivided the 368 vanA E. faecium isolates into 33 cluster types (CTs), whereas the vanB E. faecium isolate belonged to a different CT. ST203-CT859 was most prevalent (51%), followed by ST80-CT14 (22%), ST117-CT24 (6%), ST80-CT866 (4%) and ST80-CT860 (2%). Comparison with the cgMLST.org database, previous studies and personal communications with neighbouring countries revealed that the novel cluster ST203-CT859 emerged in December 2014 and spread to the south of Sweden and the Faroe Islands during 2015. Conclusions: VRE increased in Denmark during 2005-15 due to the emergence of several vanA E. faecium clones. Article in Journal/Newspaper Faroe Islands University of Southern Denmark Research Portal Journal of Antimicrobial Chemotherapy 72 8 2184 2190 |
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Open Polar |
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University of Southern Denmark Research Portal |
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ftsydanskunivpub |
language |
English |
topic |
Journal Article |
spellingShingle |
Journal Article Hammerum, Anette M Baig, Sharmin Kamel, Yasmin Roer, Louise Pinholt, Mette Gumpert, Heidi Holzknecht, Barbara Juliane Røder, Bent Justesen, Ulrik S Samulioniené, Jurgita Kjærsgaard, Mona Østergaard, Claus Holm, Anette Dzajic, Esad Søndergaard, Turid Snekloth Gaini, Shahin Edquist, Petra Alm, Erik Lilje, Berit Westh, Henrik Stegger, Marc Hasman, Henrik Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
topic_facet |
Journal Article |
description |
Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. Methods: During 2005-14, all clinical VRE isolates were tested for the presence of vanA/B/C genes by PCR. In 2015, all clinical VRE isolates were whole-genome sequenced. From the WGS data, the presence of van genes and MLST STs were extracted in silico . Core-genome MLST (cgMLST) analysis was performed for the vancomycin-resistant E. faecium isolates. Results: During 2005-15, 1043 vanA E. faecium , 25 vanB E. faecium , 4 vanA E. faecalis and 28 vanB E. faecalis were detected. The number of VRE was <50 isolates/year until 2012 to > 200 isolates/year in 2013-15. In 2015, 368 vanA E. faecium and 1 vanB E. faecium were detected along with 1 vanA E. faecalis and 1 vanB E. faecalis . cgMLST subdivided the 368 vanA E. faecium isolates into 33 cluster types (CTs), whereas the vanB E. faecium isolate belonged to a different CT. ST203-CT859 was most prevalent (51%), followed by ST80-CT14 (22%), ST117-CT24 (6%), ST80-CT866 (4%) and ST80-CT860 (2%). Comparison with the cgMLST.org database, previous studies and personal communications with neighbouring countries revealed that the novel cluster ST203-CT859 emerged in December 2014 and spread to the south of Sweden and the Faroe Islands during 2015. Conclusions: VRE increased in Denmark during 2005-15 due to the emergence of several vanA E. faecium clones. |
format |
Article in Journal/Newspaper |
author |
Hammerum, Anette M Baig, Sharmin Kamel, Yasmin Roer, Louise Pinholt, Mette Gumpert, Heidi Holzknecht, Barbara Juliane Røder, Bent Justesen, Ulrik S Samulioniené, Jurgita Kjærsgaard, Mona Østergaard, Claus Holm, Anette Dzajic, Esad Søndergaard, Turid Snekloth Gaini, Shahin Edquist, Petra Alm, Erik Lilje, Berit Westh, Henrik Stegger, Marc Hasman, Henrik |
author_facet |
Hammerum, Anette M Baig, Sharmin Kamel, Yasmin Roer, Louise Pinholt, Mette Gumpert, Heidi Holzknecht, Barbara Juliane Røder, Bent Justesen, Ulrik S Samulioniené, Jurgita Kjærsgaard, Mona Østergaard, Claus Holm, Anette Dzajic, Esad Søndergaard, Turid Snekloth Gaini, Shahin Edquist, Petra Alm, Erik Lilje, Berit Westh, Henrik Stegger, Marc Hasman, Henrik |
author_sort |
Hammerum, Anette M |
title |
Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
title_short |
Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
title_full |
Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
title_fullStr |
Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
title_full_unstemmed |
Emergence of vanA Enterococcus faecium in Denmark, 2005-15 |
title_sort |
emergence of vana enterococcus faecium in denmark, 2005-15 |
publishDate |
2017 |
url |
https://portal.findresearcher.sdu.dk/da/publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb https://doi.org/10.1093/jac/dkx138 |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
Hammerum , A M , Baig , S , Kamel , Y , Roer , L , Pinholt , M , Gumpert , H , Holzknecht , B J , Røder , B , Justesen , U S , Samulioniené , J , Kjærsgaard , M , Østergaard , C , Holm , A , Dzajic , E , Søndergaard , T S , Gaini , S , Edquist , P , Alm , E , Lilje , B , Westh , H , Stegger , M & Hasman , H 2017 , ' Emergence of vanA Enterococcus faecium in Denmark, 2005-15 ' , Journal of Antimicrobial Chemotherapy , vol. 72 , no. 8 , pp. 2184-2190 . https://doi.org/10.1093/jac/dkx138 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/aac4855d-5a06-4abe-9632-2bb822e0a2fb |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1093/jac/dkx138 |
container_title |
Journal of Antimicrobial Chemotherapy |
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72 |
container_issue |
8 |
container_start_page |
2184 |
op_container_end_page |
2190 |
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1810443218953699328 |