Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
BACKGROUND: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM 10 exposure. METHODS: Whole genome gene expression was measured in peripheral blood of 98 adul...
Published in: | Environmental Health |
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2017
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/94276035-dfcf-43dc-918b-f32d95639241 https://doi.org/10.1186/s12940-017-0292-7 https://findresearcher.sdu.dk/ws/files/135405458/Transcriptome_wide_analyses_indicate_mitochondrial_responses_to_particulate_air_pollution_exposure.pdf |
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ftsydanskunivpub:oai:sdu.dk:publications/94276035-dfcf-43dc-918b-f32d95639241 2024-04-28T08:15:41+00:00 Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte De Kok, Theo M. Vrijens, Karen 2017-08-18 application/pdf https://portal.findresearcher.sdu.dk/da/publications/94276035-dfcf-43dc-918b-f32d95639241 https://doi.org/10.1186/s12940-017-0292-7 https://findresearcher.sdu.dk/ws/files/135405458/Transcriptome_wide_analyses_indicate_mitochondrial_responses_to_particulate_air_pollution_exposure.pdf eng eng https://portal.findresearcher.sdu.dk/da/publications/94276035-dfcf-43dc-918b-f32d95639241 info:eu-repo/semantics/openAccess Winckelmans , E , Nawrot , T S , Tsamou , M , Den Hond , E , Baeyens , W , Kleinjans , J , Lefebvre , W , Van Larebeke , N , Peusens , M , Plusquin , M , Reynders , H , Schoeters , G , Vanpoucke , C , De Kok , T M & Vrijens , K 2017 , ' Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure ' , Environmental Health , vol. 16 , 87 . https://doi.org/10.1186/s12940-017-0292-7 Ambient air pollution Particulate matter Sex-specific mitochondria Transcriptome-wide analyses Air Pollutants/toxicity Environmental Monitoring Genes Mitochondrial/drug effects Humans Middle Aged Male Gene Expression Profiling Environmental Exposure Belgium Sex Factors Transcriptome/drug effects Female Aged Particulate Matter/toxicity Real-Time Polymerase Chain Reaction Cohort Studies article 2017 ftsydanskunivpub https://doi.org/10.1186/s12940-017-0292-7 2024-04-10T14:31:04Z BACKGROUND: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM 10 exposure. METHODS: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM 10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). RESULTS: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM 10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. CONCLUSIONS: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. Article in Journal/Newspaper Carbonic acid University of Southern Denmark Research Portal Environmental Health 16 1 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
topic |
Ambient air pollution Particulate matter Sex-specific mitochondria Transcriptome-wide analyses Air Pollutants/toxicity Environmental Monitoring Genes Mitochondrial/drug effects Humans Middle Aged Male Gene Expression Profiling Environmental Exposure Belgium Sex Factors Transcriptome/drug effects Female Aged Particulate Matter/toxicity Real-Time Polymerase Chain Reaction Cohort Studies |
spellingShingle |
Ambient air pollution Particulate matter Sex-specific mitochondria Transcriptome-wide analyses Air Pollutants/toxicity Environmental Monitoring Genes Mitochondrial/drug effects Humans Middle Aged Male Gene Expression Profiling Environmental Exposure Belgium Sex Factors Transcriptome/drug effects Female Aged Particulate Matter/toxicity Real-Time Polymerase Chain Reaction Cohort Studies Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte De Kok, Theo M. Vrijens, Karen Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
topic_facet |
Ambient air pollution Particulate matter Sex-specific mitochondria Transcriptome-wide analyses Air Pollutants/toxicity Environmental Monitoring Genes Mitochondrial/drug effects Humans Middle Aged Male Gene Expression Profiling Environmental Exposure Belgium Sex Factors Transcriptome/drug effects Female Aged Particulate Matter/toxicity Real-Time Polymerase Chain Reaction Cohort Studies |
description |
BACKGROUND: Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM 10 exposure. METHODS: Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM 10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). RESULTS: Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM 10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. CONCLUSIONS: In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. |
format |
Article in Journal/Newspaper |
author |
Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte De Kok, Theo M. Vrijens, Karen |
author_facet |
Winckelmans, Ellen Nawrot, Tim S. Tsamou, Maria Den Hond, Elly Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Van Larebeke, Nicolas Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte De Kok, Theo M. Vrijens, Karen |
author_sort |
Winckelmans, Ellen |
title |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_short |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_fullStr |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full_unstemmed |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_sort |
transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
publishDate |
2017 |
url |
https://portal.findresearcher.sdu.dk/da/publications/94276035-dfcf-43dc-918b-f32d95639241 https://doi.org/10.1186/s12940-017-0292-7 https://findresearcher.sdu.dk/ws/files/135405458/Transcriptome_wide_analyses_indicate_mitochondrial_responses_to_particulate_air_pollution_exposure.pdf |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
Winckelmans , E , Nawrot , T S , Tsamou , M , Den Hond , E , Baeyens , W , Kleinjans , J , Lefebvre , W , Van Larebeke , N , Peusens , M , Plusquin , M , Reynders , H , Schoeters , G , Vanpoucke , C , De Kok , T M & Vrijens , K 2017 , ' Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure ' , Environmental Health , vol. 16 , 87 . https://doi.org/10.1186/s12940-017-0292-7 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/94276035-dfcf-43dc-918b-f32d95639241 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12940-017-0292-7 |
container_title |
Environmental Health |
container_volume |
16 |
container_issue |
1 |
_version_ |
1797581118867767296 |