In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon
It is assumed that the mobilisation of a strong cellular immune response is important for the survival of Atlantic salmon infected with infectious salmon anaemia virus (ISAV). In this study, the characterisation of immune cell populations in tissues of non-ISAV infected Atlantic salmon and during th...
Published in: | Fish & Shellfish Immunology |
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2010
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/7b3fb260-eeef-11de-aefb-000ea68e967b https://doi.org/10.1016/j.fsi.2009.09.011 |
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ftsydanskunivpub:oai:sdu.dk:publications/7b3fb260-eeef-11de-aefb-000ea68e967b 2023-05-15T15:29:02+02:00 In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon Hetland, Dyveke Lem Jørgensen, Sven Martin Skjødt, Karsten Dale, Ole Bendik Falk, Knut Xu, Cheng Mikalsen, Aase B Grimholt, Unni Gjøen, Tor Press, Charles McL 2010 https://portal.findresearcher.sdu.dk/da/publications/7b3fb260-eeef-11de-aefb-000ea68e967b https://doi.org/10.1016/j.fsi.2009.09.011 eng eng info:eu-repo/semantics/restrictedAccess Hetland , D L , Jørgensen , S M , Skjødt , K , Dale , O B , Falk , K , Xu , C , Mikalsen , A B , Grimholt , U , Gjøen , T & Press , C M 2010 , ' In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon ' , Fish and Shellfish Immunology , vol. 28 , no. 1 , pp. 30-39 . https://doi.org/10.1016/j.fsi.2009.09.011 article 2010 ftsydanskunivpub https://doi.org/10.1016/j.fsi.2009.09.011 2022-08-14T08:16:35Z It is assumed that the mobilisation of a strong cellular immune response is important for the survival of Atlantic salmon infected with infectious salmon anaemia virus (ISAV). In this study, the characterisation of immune cell populations in tissues of non-ISAV infected Atlantic salmon and during the early viraemia of ISAV was undertaken. Immunohistochemical investigations of spleen, head kidney and gills using monoclonal antibodies against recombinant proteins from MHC I, II and CD8 were performed on tissues from Atlantic salmon collected day 17 post-challenge in a cohabitant infection model. The localisations of MHC I and II in control salmon were consistent with previous reports but this study presents novel observations on the distribution of CD8 labelled cell populations in Atlantic salmon including the description of significant mucosal populations in the gills. The distribution of MHC I, MHC II and CD8 positive cell populations differed between control salmon and cohabitant salmon in the early stages of ISAV infection. The changes in MHC I labelled cells differed between organs in ISAV cohabitants but all investigated organs showed a decreased presence of MHC II labelled cells. Together with a clustering of CD8 labelled cells in the head kidney and a reduced presence of CD8 labelled cells in the gills, these observations support the early mobilisation of cellular immunity in the response of Atlantic salmon to ISAV infection. However, differences between the present study and the findings from studies investigating immune gene mRNA expression during ISAV infection suggest that viral strategies to interfere with protein expression and circumvent the host immune response could be operative in the early response to ISAV infection. Article in Journal/Newspaper Atlantic salmon University of Southern Denmark Research Portal Fish & Shellfish Immunology 28 1 30 39 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
description |
It is assumed that the mobilisation of a strong cellular immune response is important for the survival of Atlantic salmon infected with infectious salmon anaemia virus (ISAV). In this study, the characterisation of immune cell populations in tissues of non-ISAV infected Atlantic salmon and during the early viraemia of ISAV was undertaken. Immunohistochemical investigations of spleen, head kidney and gills using monoclonal antibodies against recombinant proteins from MHC I, II and CD8 were performed on tissues from Atlantic salmon collected day 17 post-challenge in a cohabitant infection model. The localisations of MHC I and II in control salmon were consistent with previous reports but this study presents novel observations on the distribution of CD8 labelled cell populations in Atlantic salmon including the description of significant mucosal populations in the gills. The distribution of MHC I, MHC II and CD8 positive cell populations differed between control salmon and cohabitant salmon in the early stages of ISAV infection. The changes in MHC I labelled cells differed between organs in ISAV cohabitants but all investigated organs showed a decreased presence of MHC II labelled cells. Together with a clustering of CD8 labelled cells in the head kidney and a reduced presence of CD8 labelled cells in the gills, these observations support the early mobilisation of cellular immunity in the response of Atlantic salmon to ISAV infection. However, differences between the present study and the findings from studies investigating immune gene mRNA expression during ISAV infection suggest that viral strategies to interfere with protein expression and circumvent the host immune response could be operative in the early response to ISAV infection. |
format |
Article in Journal/Newspaper |
author |
Hetland, Dyveke Lem Jørgensen, Sven Martin Skjødt, Karsten Dale, Ole Bendik Falk, Knut Xu, Cheng Mikalsen, Aase B Grimholt, Unni Gjøen, Tor Press, Charles McL |
spellingShingle |
Hetland, Dyveke Lem Jørgensen, Sven Martin Skjødt, Karsten Dale, Ole Bendik Falk, Knut Xu, Cheng Mikalsen, Aase B Grimholt, Unni Gjøen, Tor Press, Charles McL In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
author_facet |
Hetland, Dyveke Lem Jørgensen, Sven Martin Skjødt, Karsten Dale, Ole Bendik Falk, Knut Xu, Cheng Mikalsen, Aase B Grimholt, Unni Gjøen, Tor Press, Charles McL |
author_sort |
Hetland, Dyveke Lem |
title |
In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
title_short |
In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
title_full |
In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
title_fullStr |
In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
title_full_unstemmed |
In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon |
title_sort |
in situ localisation of major histocompatibility complex class i and class ii and cd8 positive cells in infectious salmon anaemia virus (isav)-infected atlantic salmon |
publishDate |
2010 |
url |
https://portal.findresearcher.sdu.dk/da/publications/7b3fb260-eeef-11de-aefb-000ea68e967b https://doi.org/10.1016/j.fsi.2009.09.011 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Hetland , D L , Jørgensen , S M , Skjødt , K , Dale , O B , Falk , K , Xu , C , Mikalsen , A B , Grimholt , U , Gjøen , T & Press , C M 2010 , ' In situ localisation of major histocompatibility complex class I and class II and CD8 positive cells in infectious salmon anaemia virus (ISAV)-infected Atlantic salmon ' , Fish and Shellfish Immunology , vol. 28 , no. 1 , pp. 30-39 . https://doi.org/10.1016/j.fsi.2009.09.011 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2009.09.011 |
container_title |
Fish & Shellfish Immunology |
container_volume |
28 |
container_issue |
1 |
container_start_page |
30 |
op_container_end_page |
39 |
_version_ |
1766359402772692992 |