Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells
The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. The present...
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/5de86e37-1843-4fff-9ceb-66f151f909c1 https://doi.org/10.1128/IAI.00556-17 https://findresearcher.sdu.dk/ws/files/142377126/Acquired_Protective_Immunity_in_Atlantic_Salmon_Salmo_salar_against_the_Myxozoan_Kudoa_thyrsites_Involves_Induction_of_MHII_CD83_Antigen_Presenting_Cells.pdf |
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ftsydanskunivpub:oai:sdu.dk:publications/5de86e37-1843-4fff-9ceb-66f151f909c1 2023-05-15T15:31:14+02:00 Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells Braden, Laura M Rasmussen, Karina J Purcell, Sara L Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K Jones, Simon R M Fast, Mark D 2018-01 application/pdf https://portal.findresearcher.sdu.dk/da/publications/5de86e37-1843-4fff-9ceb-66f151f909c1 https://doi.org/10.1128/IAI.00556-17 https://findresearcher.sdu.dk/ws/files/142377126/Acquired_Protective_Immunity_in_Atlantic_Salmon_Salmo_salar_against_the_Myxozoan_Kudoa_thyrsites_Involves_Induction_of_MHII_CD83_Antigen_Presenting_Cells.pdf eng eng info:eu-repo/semantics/openAccess Braden , L M , Rasmussen , K J , Purcell , S L , Ellis , L , Mahony , A , Cho , S , Whyte , S K , Jones , S R M & Fast , M D 2018 , ' Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells ' , Infection and Immunity , vol. 86 , no. 1 , e00556-17 . https://doi.org/10.1128/IAI.00556-17 Adaptive Immunity/immunology Animals Antigen-Presenting Cells/immunology Antigens CD/immunology Aquaculture/methods B-Lymphocytes/immunology Dendritic Cells/immunology Fish Diseases/immunology Host-Parasite Interactions/immunology Immunoglobulins/immunology Macrophages/immunology Membrane Glycoproteins/immunology Muscle Cells/immunology Muscle Skeletal/immunology Myxozoa/immunology Parasitic Diseases Animal/immunology Salmo salar/immunology Salmon/immunology T-Lymphocytes/immunology article 2018 ftsydanskunivpub https://doi.org/10.1128/IAI.00556-17 2022-08-14T09:57:30Z The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. The present work sought to characterize the immune response in Atlantic salmon during infection, recovery, and reexposure to K. thyrsites After exposure to infective seawater, infected and uninfected smolts were sampled three times over 4,275 degree-days. Histological analysis revealed infection severity decreased over time in exposed fish, while in controls there was no evidence of infection. Following a secondary exposure of all fish, severity of infection in the controls was similar to that measured in exposed fish at the first sampling time but was significantly reduced in reexposed fish, suggesting the acquisition of protective immunity. Using immunohistochemistry, we detected a population of MHIIβ+ cells in infected muscle that followed a pattern of abundance concordant with parasite prevalence. Infiltration of these cells into infected myocytes preceded destruction of the plasmodium and dissemination of myxospores. Dual labeling indicated a majority of these cells were CD83+/MHIIβ+ Using reverse transcription-quantitative PCR, we detected significant induction of cellular effectors, including macrophage/dendritic cells (mhii/cd83/mcsf), B cells (igm/igt), and cytotoxic T cells (cd8/nkl), in the musculature of infected fish. These data support a role for cellular effectors such as antigen-presenting cells (monocyte/macrophage and dendritic cells) along with B and T cells in the acquired protective immune response of Atlantic salmon against K. thyrsites. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Southern Denmark Research Portal Pacific Infection and Immunity 86 1 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
topic |
Adaptive Immunity/immunology Animals Antigen-Presenting Cells/immunology Antigens CD/immunology Aquaculture/methods B-Lymphocytes/immunology Dendritic Cells/immunology Fish Diseases/immunology Host-Parasite Interactions/immunology Immunoglobulins/immunology Macrophages/immunology Membrane Glycoproteins/immunology Muscle Cells/immunology Muscle Skeletal/immunology Myxozoa/immunology Parasitic Diseases Animal/immunology Salmo salar/immunology Salmon/immunology T-Lymphocytes/immunology |
spellingShingle |
Adaptive Immunity/immunology Animals Antigen-Presenting Cells/immunology Antigens CD/immunology Aquaculture/methods B-Lymphocytes/immunology Dendritic Cells/immunology Fish Diseases/immunology Host-Parasite Interactions/immunology Immunoglobulins/immunology Macrophages/immunology Membrane Glycoproteins/immunology Muscle Cells/immunology Muscle Skeletal/immunology Myxozoa/immunology Parasitic Diseases Animal/immunology Salmo salar/immunology Salmon/immunology T-Lymphocytes/immunology Braden, Laura M Rasmussen, Karina J Purcell, Sara L Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K Jones, Simon R M Fast, Mark D Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
topic_facet |
Adaptive Immunity/immunology Animals Antigen-Presenting Cells/immunology Antigens CD/immunology Aquaculture/methods B-Lymphocytes/immunology Dendritic Cells/immunology Fish Diseases/immunology Host-Parasite Interactions/immunology Immunoglobulins/immunology Macrophages/immunology Membrane Glycoproteins/immunology Muscle Cells/immunology Muscle Skeletal/immunology Myxozoa/immunology Parasitic Diseases Animal/immunology Salmo salar/immunology Salmon/immunology T-Lymphocytes/immunology |
description |
The histozoic myxozoan parasite Kudoa thyrsites causes postmortem myoliquefaction and is responsible for economic losses to salmon aquaculture in the Pacific Northwest. Despite its importance, little is known about the host-parasite relationship, including the host response to infection. The present work sought to characterize the immune response in Atlantic salmon during infection, recovery, and reexposure to K. thyrsites After exposure to infective seawater, infected and uninfected smolts were sampled three times over 4,275 degree-days. Histological analysis revealed infection severity decreased over time in exposed fish, while in controls there was no evidence of infection. Following a secondary exposure of all fish, severity of infection in the controls was similar to that measured in exposed fish at the first sampling time but was significantly reduced in reexposed fish, suggesting the acquisition of protective immunity. Using immunohistochemistry, we detected a population of MHIIβ+ cells in infected muscle that followed a pattern of abundance concordant with parasite prevalence. Infiltration of these cells into infected myocytes preceded destruction of the plasmodium and dissemination of myxospores. Dual labeling indicated a majority of these cells were CD83+/MHIIβ+ Using reverse transcription-quantitative PCR, we detected significant induction of cellular effectors, including macrophage/dendritic cells (mhii/cd83/mcsf), B cells (igm/igt), and cytotoxic T cells (cd8/nkl), in the musculature of infected fish. These data support a role for cellular effectors such as antigen-presenting cells (monocyte/macrophage and dendritic cells) along with B and T cells in the acquired protective immune response of Atlantic salmon against K. thyrsites. |
format |
Article in Journal/Newspaper |
author |
Braden, Laura M Rasmussen, Karina J Purcell, Sara L Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K Jones, Simon R M Fast, Mark D |
author_facet |
Braden, Laura M Rasmussen, Karina J Purcell, Sara L Ellis, Lauren Mahony, Amelia Cho, Steven Whyte, Shona K Jones, Simon R M Fast, Mark D |
author_sort |
Braden, Laura M |
title |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
title_short |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
title_full |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
title_fullStr |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
title_full_unstemmed |
Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells |
title_sort |
acquired protective immunity in atlantic salmon salmo salar against the myxozoan kudoa thyrsites involves induction of mhiiβ+ cd83+ antigen-presenting cells |
publishDate |
2018 |
url |
https://portal.findresearcher.sdu.dk/da/publications/5de86e37-1843-4fff-9ceb-66f151f909c1 https://doi.org/10.1128/IAI.00556-17 https://findresearcher.sdu.dk/ws/files/142377126/Acquired_Protective_Immunity_in_Atlantic_Salmon_Salmo_salar_against_the_Myxozoan_Kudoa_thyrsites_Involves_Induction_of_MHII_CD83_Antigen_Presenting_Cells.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Braden , L M , Rasmussen , K J , Purcell , S L , Ellis , L , Mahony , A , Cho , S , Whyte , S K , Jones , S R M & Fast , M D 2018 , ' Acquired Protective Immunity in Atlantic Salmon Salmo salar against the Myxozoan Kudoa thyrsites Involves Induction of MHIIβ+ CD83+ Antigen-Presenting Cells ' , Infection and Immunity , vol. 86 , no. 1 , e00556-17 . https://doi.org/10.1128/IAI.00556-17 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/IAI.00556-17 |
container_title |
Infection and Immunity |
container_volume |
86 |
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1 |
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