Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon.
We recently showed that a series of tight junction proteins of the claudin family are regulated in the gill of salmon during salinity acclimation. The aim of the present study was to investigate the role of cortisol, growth hormone (GH) and prolactin (PRL) on regulation of expression of these isofor...
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Online Access: | https://portal.findresearcher.sdu.dk/da/publications/284df950-0749-11df-aefb-000ea68e967b https://doi.org/10.1016/j.ygcen.2009.04.020 |
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ftsydanskunivpub:oai:sdu.dk:publications/284df950-0749-11df-aefb-000ea68e967b 2024-05-19T07:37:48+00:00 Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. Tipsmark, Christian Kølbæk Jørgensen, Charlotte Brande-Lavridsen, Nanna Engelund, Morten Olsen, Jesper H. Madsen, Steffen 2009 https://portal.findresearcher.sdu.dk/da/publications/284df950-0749-11df-aefb-000ea68e967b https://doi.org/10.1016/j.ygcen.2009.04.020 eng eng https://portal.findresearcher.sdu.dk/da/publications/284df950-0749-11df-aefb-000ea68e967b info:eu-repo/semantics/restrictedAccess Tipsmark , C K , Jørgensen , C , Brande-Lavridsen , N , Engelund , M , Olsen , J H & Madsen , S 2009 , ' Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. ' , General and Comparative Endocrinology , vol. 163 , pp. 270-277 . https://doi.org/10.1016/j.ygcen.2009.04.020 Receptor Salmo salar Tight junction teleost article 2009 ftsydanskunivpub https://doi.org/10.1016/j.ygcen.2009.04.020 2024-04-24T00:20:45Z We recently showed that a series of tight junction proteins of the claudin family are regulated in the gill of salmon during salinity acclimation. The aim of the present study was to investigate the role of cortisol, growth hormone (GH) and prolactin (PRL) on regulation of expression of these isoforms. Experiments on primary cultures of gill tissue showed that cortisol stimulates claudin 10e, 27a and 30 mRNA levels while no significant effects were observed on claudin 28a and 28b. The associated receptor signalling pathway was examined using glucocorticoid and mineralocorticoid receptor antagonists RU486 and spironolactone, respectively. The observed in vitro responses were blocked by RU486, suggesting the involvement of a glucocorticoid type receptor. Injections of FW salmon with cortisol increased the expression of claudin 10e, 27a, and 30 but did not affect claudin 28a and 28b significantly. While GH had no effect on its own, the combination of GH and cortisol reduced claudin 28b levels. Injection of SW salmon with PRL selectively increased the expression of claudin 28a but had no effect on the other examined isoforms. The data shows that FW- (27a and 30) and SW-induced (10e) claudins are all stimulated by cortisol while the major osmoregulatory hormones GH and PRL had no effect on these salinity sensitive isoforms. This suggests that other hormones and/or osmotic conditions interact with cortisol to determine claudin composition in the gill. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Southern Denmark Research Portal General and Comparative Endocrinology 163 3 270 277 |
institution |
Open Polar |
collection |
University of Southern Denmark Research Portal |
op_collection_id |
ftsydanskunivpub |
language |
English |
topic |
Receptor Salmo salar Tight junction teleost |
spellingShingle |
Receptor Salmo salar Tight junction teleost Tipsmark, Christian Kølbæk Jørgensen, Charlotte Brande-Lavridsen, Nanna Engelund, Morten Olsen, Jesper H. Madsen, Steffen Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
topic_facet |
Receptor Salmo salar Tight junction teleost |
description |
We recently showed that a series of tight junction proteins of the claudin family are regulated in the gill of salmon during salinity acclimation. The aim of the present study was to investigate the role of cortisol, growth hormone (GH) and prolactin (PRL) on regulation of expression of these isoforms. Experiments on primary cultures of gill tissue showed that cortisol stimulates claudin 10e, 27a and 30 mRNA levels while no significant effects were observed on claudin 28a and 28b. The associated receptor signalling pathway was examined using glucocorticoid and mineralocorticoid receptor antagonists RU486 and spironolactone, respectively. The observed in vitro responses were blocked by RU486, suggesting the involvement of a glucocorticoid type receptor. Injections of FW salmon with cortisol increased the expression of claudin 10e, 27a, and 30 but did not affect claudin 28a and 28b significantly. While GH had no effect on its own, the combination of GH and cortisol reduced claudin 28b levels. Injection of SW salmon with PRL selectively increased the expression of claudin 28a but had no effect on the other examined isoforms. The data shows that FW- (27a and 30) and SW-induced (10e) claudins are all stimulated by cortisol while the major osmoregulatory hormones GH and PRL had no effect on these salinity sensitive isoforms. This suggests that other hormones and/or osmotic conditions interact with cortisol to determine claudin composition in the gill. |
format |
Article in Journal/Newspaper |
author |
Tipsmark, Christian Kølbæk Jørgensen, Charlotte Brande-Lavridsen, Nanna Engelund, Morten Olsen, Jesper H. Madsen, Steffen |
author_facet |
Tipsmark, Christian Kølbæk Jørgensen, Charlotte Brande-Lavridsen, Nanna Engelund, Morten Olsen, Jesper H. Madsen, Steffen |
author_sort |
Tipsmark, Christian Kølbæk |
title |
Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
title_short |
Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
title_full |
Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
title_fullStr |
Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
title_full_unstemmed |
Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. |
title_sort |
effects of cortisol, growth hormone and prolactin on gill claudin expression in atlantic salmon. |
publishDate |
2009 |
url |
https://portal.findresearcher.sdu.dk/da/publications/284df950-0749-11df-aefb-000ea68e967b https://doi.org/10.1016/j.ygcen.2009.04.020 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Tipsmark , C K , Jørgensen , C , Brande-Lavridsen , N , Engelund , M , Olsen , J H & Madsen , S 2009 , ' Effects of cortisol, growth hormone and prolactin on gill claudin expression in Atlantic salmon. ' , General and Comparative Endocrinology , vol. 163 , pp. 270-277 . https://doi.org/10.1016/j.ygcen.2009.04.020 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/284df950-0749-11df-aefb-000ea68e967b |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2009.04.020 |
container_title |
General and Comparative Endocrinology |
container_volume |
163 |
container_issue |
3 |
container_start_page |
270 |
op_container_end_page |
277 |
_version_ |
1799477178314784768 |