Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD)
The heart is considered the powerhouse of the cardiovascular system. Heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) are cardiac diseases of marine farmed Atlantic salmon (Salmo salar) which commonly affect the heart in addition to the skeletal...
Published in: | Veterinary Immunology and Immunopathology |
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2013
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ftsydanskunivpub:oai:sdu.dk:publications/2517f4cd-7cef-42ee-af59-ca5f8fa0d4f2 2024-09-15T17:56:03+00:00 Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) Yousaf, Muhammad Naveed Koppang, Erling Olaf Skjødt, Karsten Hordvik, Ivar Zou, Jun Secombes, Chris Powell, Mark D 2013 https://portal.findresearcher.sdu.dk/da/publications/2517f4cd-7cef-42ee-af59-ca5f8fa0d4f2 https://doi.org/10.1016/j.vetimm.2012.10.004 eng eng https://portal.findresearcher.sdu.dk/da/publications/2517f4cd-7cef-42ee-af59-ca5f8fa0d4f2 info:eu-repo/semantics/restrictedAccess Yousaf , M N , Koppang , E O , Skjødt , K , Hordvik , I , Zou , J , Secombes , C & Powell , M D 2013 , ' Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) ' , Veterinary Immunology and Immunopathology , vol. 151 , no. 1-2 , pp. 49-62 . https://doi.org/10.1016/j.vetimm.2012.10.004 article 2013 ftsydanskunivpub https://doi.org/10.1016/j.vetimm.2012.10.004 2024-07-22T23:48:46Z The heart is considered the powerhouse of the cardiovascular system. Heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) are cardiac diseases of marine farmed Atlantic salmon (Salmo salar) which commonly affect the heart in addition to the skeletal muscle, liver and pancreas. The main findings of these diseases are necrosis and inflammatory cells infiltrates affecting different regions of the heart. In order to better characterize the cardiac pathology, study of the inflammatory cell characteristics and cell cycle protein expression was undertaken by immunohistochemistry. Immunohistochemistry was performed on paraffin embedded hearts from confirmed diseased cases applying specific antibodies. The inflammatory cells were predominantly CD3(+) T lymphocytes. The PD diseased hearts exhibited moderate hypoxia inducible factor-1α (HIF1α) immuno-reaction that suggested tissue hypoxia while recombinant tumor necrosis factor-α (rTNFα) antibody identified putative macrophages and eosinophilic granulocytes (EGCs) in addition to endocardial cells around lesions. There were strong to low levels of major histocompatibility complex (MHC) class II immunostaining in the diseased hearts associated with macrophage-like and lymphocyte-like cells. The diseased hearts expressed strong to low levels of apoptotic cells identified by caspase 3 and terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining. The strong signals for proliferative cell nuclear antigen (PCNA) and TUNEL, and moderate levels of caspase 3 immuno-reactivity suggested a high cell turnover where DNA damage/repair might be occurring in the diseased hearts. Interestingly, the apparently similar cardiac diseases exhibited differences in the immunopathological responses in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Southern Denmark Research Portal Veterinary Immunology and Immunopathology 151 1-2 49 62 |
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Open Polar |
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University of Southern Denmark Research Portal |
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ftsydanskunivpub |
language |
English |
description |
The heart is considered the powerhouse of the cardiovascular system. Heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) are cardiac diseases of marine farmed Atlantic salmon (Salmo salar) which commonly affect the heart in addition to the skeletal muscle, liver and pancreas. The main findings of these diseases are necrosis and inflammatory cells infiltrates affecting different regions of the heart. In order to better characterize the cardiac pathology, study of the inflammatory cell characteristics and cell cycle protein expression was undertaken by immunohistochemistry. Immunohistochemistry was performed on paraffin embedded hearts from confirmed diseased cases applying specific antibodies. The inflammatory cells were predominantly CD3(+) T lymphocytes. The PD diseased hearts exhibited moderate hypoxia inducible factor-1α (HIF1α) immuno-reaction that suggested tissue hypoxia while recombinant tumor necrosis factor-α (rTNFα) antibody identified putative macrophages and eosinophilic granulocytes (EGCs) in addition to endocardial cells around lesions. There were strong to low levels of major histocompatibility complex (MHC) class II immunostaining in the diseased hearts associated with macrophage-like and lymphocyte-like cells. The diseased hearts expressed strong to low levels of apoptotic cells identified by caspase 3 and terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) staining. The strong signals for proliferative cell nuclear antigen (PCNA) and TUNEL, and moderate levels of caspase 3 immuno-reactivity suggested a high cell turnover where DNA damage/repair might be occurring in the diseased hearts. Interestingly, the apparently similar cardiac diseases exhibited differences in the immunopathological responses in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Yousaf, Muhammad Naveed Koppang, Erling Olaf Skjødt, Karsten Hordvik, Ivar Zou, Jun Secombes, Chris Powell, Mark D |
spellingShingle |
Yousaf, Muhammad Naveed Koppang, Erling Olaf Skjødt, Karsten Hordvik, Ivar Zou, Jun Secombes, Chris Powell, Mark D Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
author_facet |
Yousaf, Muhammad Naveed Koppang, Erling Olaf Skjødt, Karsten Hordvik, Ivar Zou, Jun Secombes, Chris Powell, Mark D |
author_sort |
Yousaf, Muhammad Naveed |
title |
Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
title_short |
Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
title_full |
Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
title_fullStr |
Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
title_full_unstemmed |
Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) |
title_sort |
comparative cardiac pathological changes of atlantic salmon (salmo salar l.) affected with heart and skeletal muscle inflammation (hsmi), cardiomyopathy syndrome (cms) and pancreas disease (pd) |
publishDate |
2013 |
url |
https://portal.findresearcher.sdu.dk/da/publications/2517f4cd-7cef-42ee-af59-ca5f8fa0d4f2 https://doi.org/10.1016/j.vetimm.2012.10.004 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Yousaf , M N , Koppang , E O , Skjødt , K , Hordvik , I , Zou , J , Secombes , C & Powell , M D 2013 , ' Comparative cardiac pathological changes of Atlantic salmon (Salmo salar L.) affected with heart and skeletal muscle inflammation (HSMI), cardiomyopathy syndrome (CMS) and pancreas disease (PD) ' , Veterinary Immunology and Immunopathology , vol. 151 , no. 1-2 , pp. 49-62 . https://doi.org/10.1016/j.vetimm.2012.10.004 |
op_relation |
https://portal.findresearcher.sdu.dk/da/publications/2517f4cd-7cef-42ee-af59-ca5f8fa0d4f2 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.vetimm.2012.10.004 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
151 |
container_issue |
1-2 |
container_start_page |
49 |
op_container_end_page |
62 |
_version_ |
1810432271723790336 |